High levels of macrolide-resistant in Queensland, Australia Open Access

Abstract

The macrolide azithromycin is recommended for treatment of infection; however, strains possessing macrolide resistance-mediating mutations (MRMMs) are increasingly being reported. Here, we used the SpeeDx ResistancePlus MG kit, which provides simultaneous detection of and MRMMs, to assess MRMM carriage among infections in Queensland, Australia. Performance characteristics of the ResistancePlus MG kit for detection were compared to in-house PCR. Available PCR-positive (=67) and negative (=281) samples from the years 2011 to 2017 were tested using the SpeeDx ResistancePlus MG kit. In total, 63.6 % -positive samples were indicated to harbour MRMMs. The ResistancePlus MG method provided sensitivity and specificity of 97 and 99.6 % respectively compared to in-house PCR for detection. Such high levels of macrolide-resistant raise further concerns over future use of azithromycin for treatment of infection.

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2017-10-01
2024-03-28
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