1887

Abstract

Molecular typing is an important tool for control and prevention of infection. A suitable molecular typing method for epidemiological investigation must be easy to perform, highly reproducible, inexpensive, rapid and easy to interpret. In this study, two molecular typing methods including the conventional PCR-sequencing method and high resolution melting (HRM) analysis were used for staphylococcal protein A () typing of 30 Methicillin-resistant (MRSA) isolates recovered from clinical samples. Based on PCR-sequencing method results, 16 different types were identified among the 30 MRSA isolates. Among the 16 different types, 14 types separated by HRM method. Two types including t4718 and t2894 were not separated from each other. According to our results, typing based on HRM analysis method is very rapid, easy to perform and cost-effective, but this method must be standardized for different regions, types, and real-time machinery.

Keyword(s): HRM , MRSA , sequencing and Spa type
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/content/journal/jmm/10.1099/jmm.0.000574
2017-09-01
2020-01-18
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