Molecular evidence for the absence of an association between and respiratory diseases Free

Abstract

has been implicated in respiratory diseases. This study aimed to unveil the aetiological role of this bacterium in community-acquired pneumonia (CAP) and bronchitis in Jordanian adults. Nasopharyngeal samples were collected from 98 CAP or bronchitis patients and 96 control individuals, and tested for nucleic acids using a PCR assay. The overall prevalence of the bacterial DNA in patients was markedly high and reached 57.1 %. Intriguingly, DNA was detected in 62.5 % of the nasopharyngeal swabs collected from apparently healthy controls (>0.05). The DNA positivity in the bronchitis and CAP subgroups was 57.7 and 56.9 %, respectively, percentages that are approximately comparable to the DNA positivity assessed for the entire patient population. is most likely not a causative agent of CAP or bronchitis, despite its remarkable high prevalence. This organism, in the nasopharynx, is potentially harmless to the host and may coexist in a commensal relationship.

Keyword(s): bronchitis , Jordan , PCR , pneumonia , prevalence and Simkania
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000564
2017-09-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/66/9/1324.html?itemId=/content/journal/jmm/10.1099/jmm.0.000564&mimeType=html&fmt=ahah

References

  1. Kahane S, Gonen R, Sayada C, Elion J, Friedman MG. Description and partial characterization of a new Chlamydia-like microorganism. FEMS Microbiol Lett 1993; 109:329–333 [View Article][PubMed]
    [Google Scholar]
  2. Kahane S, Greenberg D, Newman N, Dvoskin B, Friedman MG. Domestic water supplies as a possible source of infection with Simkania. J Infect 2007; 54:75–81 [View Article][PubMed]
    [Google Scholar]
  3. Al-Younes HM, Paldanius M. High seroprevalence of Simkania negevensis in Jordan. Braz J Microbiol 2015; 45:1433–1437 [View Article][PubMed]
    [Google Scholar]
  4. Donati M, Fiani N, di Francesco A, di Paolo M, Vici M et al. IgG and IgA response to Simkania negevensis in sera of patients with respiratory and gastrointestinal symptoms. New Microbiol 2013; 36:303–306[PubMed]
    [Google Scholar]
  5. Friedman MG, Dvoskin B, Kahane S. Infections with the Chlamydia-like microorganism Simkania negevensis, a possible emerging pathogen. Microbes Infect 2003; 5:1013–1021 [View Article][PubMed]
    [Google Scholar]
  6. Friedman MG, Galil A, Greenberg S, Kahane S. Seroprevalence of IgG antibodies to the Chlamydia-like microorganism 'Simkania Z' by ELISA. Epidemiol Infect 1999; 122:117–123 [View Article][PubMed]
    [Google Scholar]
  7. Friedman MG, Kahane S, Dvoskin B, Hartley JW. Detection of Simkania negevensis by culture, PCR, and serology in respiratory tract infection in Cornwall, UK. J Clin Pathol 2006; 59:331–333 [View Article][PubMed]
    [Google Scholar]
  8. Johnsen S, Birkebaek N, Andersen PL, Emil C, Jensen JS et al. Indirect immunofluorescence and real time PCR for detection of Simkania negevensis infection in Danish adults with persistent cough and in healthy controls. Scand J Infect Dis 2005; 37:251–255[PubMed]
    [Google Scholar]
  9. Korppi M, Paldanius M, Hyvärinen A, Nevalainen A. Simkania negevensis and newly diagnosed asthma: a case-control study in 1- to 6-year-old children. Respirology 2006; 11:80–83 [View Article][PubMed]
    [Google Scholar]
  10. Yamaguchi T, Yamazaki T, Inoue M, Mashida C, Kawagoe K et al. Prevalence of antibodies against Simkania negevensis in a healthy Japanese population determined by the microimmunofluorescence test. FEMS Immunol Med Microbiol 2005; 43:21–27 [View Article][PubMed]
    [Google Scholar]
  11. Fasoli L, Paldanius M, Don M, Valent F, Vetrugno L et al. Simkania negevensis in community-acquired pneumonia in Italian children. Scand J Infect Dis 2008; 40:269–272 [View Article][PubMed]
    [Google Scholar]
  12. Greenberg D, Banerji A, Friedman MG, Chiu CH, Kahane S. High rate of Simkania negevensis among Canadian infants hospitalized with lower respiratory tract infections. Scand J Infect Dis 2003; 35:506–508 [View Article][PubMed]
    [Google Scholar]
  13. Heiskanen-Kosma T, Paldanius M, Korppi M. Simkania negevensis may be a true cause of community acquired pneumonia in children. Scand J Infect Dis 2008; 40:127–130 [View Article][PubMed]
    [Google Scholar]
  14. Kahane S, Greenberg D, Friedman MG, Haikin H, Dagan R. High prevalence of “Simkania Z,” a novel Chlamydia-like bacterium, in infants with acute bronchiolitis. J Infect Dis 1998; 177:1425–1429 [View Article][PubMed]
    [Google Scholar]
  15. Lieberman D, Dvoskin B, Lieberman DV, Kahane S, Friedman MG. Serological evidence of acute infection with the Chlamydia-like microorganism Simkania negevensis (Z) in acute exacerbation of chronic obstructive pulmonary disease. Eur J Clin Microbiol Infect Dis 2002; 21:307–309 [View Article][PubMed]
    [Google Scholar]
  16. Lieberman D, Kahane S, Lieberman D, Friedman MG. Pneumonia with serological evidence of acute infection with the Chlamydia-like microorganism “Z”. Am J Respir Crit Care Med 1997; 156:578–582 [View Article][PubMed]
    [Google Scholar]
  17. Nascimento-Carvalho CM, Cardoso MR, Paldanius M, Barral A, Araújo-Neto CA et al. Simkania negevensis infection among Brazilian children hospitalized with community-acquired pneumonia. J Infect 2009; 58:250–253 [View Article][PubMed]
    [Google Scholar]
  18. Ossewaarde JM, Meijer A. Molecular evidence for the existence of additional members of the order Chlamydiales. Microbiology 1999; 145:411–417 [View Article][PubMed]
    [Google Scholar]
  19. Kumar S, Kohlhoff SA, Gelling M, Roblin PM, Kutlin A et al. Infection with Simkania negevensis in Brooklyn, New York. Pediatr Infect Dis J 2005; 24:989–992 [View Article][PubMed]
    [Google Scholar]
  20. Kose M, Ekinci D, Gokahmetoglu S, Elmas T, Öztürk MK. Simkania negevensis: is it a real respiratory pathogen?. Acta Microbiol Immunol Hung 2015; 62:161–166 [View Article][PubMed]
    [Google Scholar]
  21. Niemi S, Greub G, Puolakkainen M. Chlamydia-related bacteria in respiratory samples in Finland. Microbes Infect 2011; 13:824–827 [View Article][PubMed]
    [Google Scholar]
  22. Al-Aydie SN, Obeidat NM, Al-Younes HM. Role of Chlamydia pneumoniae in community-acquired pneumonia in hospitalized Jordanian adults. J Infect Dev Ctries 2016; 10:227–236 [View Article][PubMed]
    [Google Scholar]
  23. Vouga M, Baud D, Greub G. Simkania negevensis, an insight into the biology and clinical importance of a novel member of the Chlamydiales order. Crit Rev Microbiol 2017; 43:62–80 [View Article][PubMed]
    [Google Scholar]
  24. Mehlitz A, Karunakaran K, Herweg JA, Krohne G, van de Linde S et al. The chlamydial organism Simkania negevensis forms ER vacuole contact sites and inhibits ER-stress. Cell Microbiol 2014; 16:1224–1243 [View Article][PubMed]
    [Google Scholar]
  25. Herweg JA, Pons V, Becher D, Hecker M, Krohne G et al. Proteomic analysis of the Simkania-containing vacuole: the central role of retrograde transport. Mol Microbiol 2016; 99:151–171 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.000564
Loading
/content/journal/jmm/10.1099/jmm.0.000564
Loading

Data & Media loading...

Most cited Most Cited RSS feed