Effects of tea extracts on the colonization behaviour of species: attachment inhibition and biofilm enhancement Free

Abstract

We assessed the effects of four different types of tea extracts (green, oolong, black and pu-erh tea) on cellular surface properties (hydrophobicity and auto-aggregation) and the colonization attributes (attachment and biofilm formation) of four strains of and three strains of .

The cellular surface properties were determined using spectrophotometry. The colonization activities were quantified using colorimetric viability assays and visualized using scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM).

The tea extracts, in general, reduced the hydrophobicity (by 8–66 %) and auto-aggregation (by 20–65 %), and inhibited the attachment of two strains (by 41–88 %). Tea extracts enhanced the biofilm formation of one and two strains (by 1.4–7.5-fold). The observed reduction in hydrophobicity strongly correlated with the reduction in attachment of the two strains (<0.05). The ultrastructural images of the tea-treated biofilm cells demonstrated central indentations, although they remained viable.

The tea extracts have the ability to retard adhesion to glass surfaces, possibly by reducing fungal cellular hydrophobicity, whilst paradoxically promoting biofilm formation. In practical terms, therefore, consumption of tea beverages appears to have a complex effect on oral candidal colonization.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000555
2017-08-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/66/8/1244.html?itemId=/content/journal/jmm/10.1099/jmm.0.000555&mimeType=html&fmt=ahah

References

  1. Samaranayake LP. Host factors and oral candidosis. In Samaranayake LP, MacFarlane TW. (editors) Oral Candidosis London: Butterworth; 1990 pp. 66–103
    [Google Scholar]
  2. Calderone RA. Candida and Candidiasis Washington, DC: ASM Press; 2002
    [Google Scholar]
  3. Denning DW, Donnelly JP, Hellreigel KP, Ito J, Martino P et al. Antifungal prophylaxis during neutropenia or allogeneic bone marrow transplantation: what is the state of the art? Ad HOC working group. Chemotherapy 1992; 38:43–49 [View Article][PubMed]
    [Google Scholar]
  4. Reichart PA, Samaranayake LP, Samaranayake YH, Grote M, Pow E et al. High oral prevalence of Candida krusei in leprosy patients in northern Thailand. J Clin Microbiol 2002; 40:4479–4485 [View Article][PubMed]
    [Google Scholar]
  5. Perlroth J, Choi B, Spellberg B. Nosocomial fungal infections: epidemiology, diagnosis, and treatment. Med Mycol 2007; 45:321–346 [View Article][PubMed]
    [Google Scholar]
  6. Thein ZM, Seneviratne CJ, Samaranayake YH, Samaranayake LP. Community lifestyle of Candida in mixed biofilms: a mini review. Mycoses 2009; 52:467–475 [View Article][PubMed]
    [Google Scholar]
  7. Cannon RD, Chaffin WL. Colonization is a crucial factor in oral candidiasis. J Dent Educ 2001; 65:785–787[PubMed]
    [Google Scholar]
  8. Grimaudo NJ, Nesbitt WE. Coaggregation of Candida albicans with oral Fusobacterium species. Oral Microbiol Immunol 1997; 12:168–173 [View Article][PubMed]
    [Google Scholar]
  9. Lamont R, Jenkinson H. Adhesion as an ecological determinant in the oral cavity. In Kuramitsu HK, Ellen RP. (editors) Oral Bacterial Ecology: The Molecular Basis Wymondham: Horizon Scientific Press; 2000 pp. 131–168
    [Google Scholar]
  10. Busscher HJ, Weerkamp AH, van der Mei HC, van Pelt AW, de Jong HP et al. Measurement of the surface free energy of bacterial cell surfaces and its relevance for adhesion. Appl Environ Microbiol 1984; 48:980–983[PubMed]
    [Google Scholar]
  11. Chen Y, Busscher HJ, van der Mei HC, Norde W. Statistical analysis of long- and short-range forces involved in bacterial adhesion to substratum surfaces as measured using atomic force microscopy. Appl Environ Microbiol 2011; 77:5065–5070 [View Article][PubMed]
    [Google Scholar]
  12. van der Mei HC, Roseberg M, Busscher HJ. Assessment of microbial cell surface hydrophobicity. In Mozes N, Handley PS, Busscher HJ, Rouxhet PG. (editors) Microbial Cell Surface Analysis: Structural and Physicochemical Methods New York: VCH Publishers; 1991 pp. 265–289
    [Google Scholar]
  13. Wang Y, Lee SM, Dykes GA. Potential mechanisms for the effects of tea extracts on the attachment, biofilm formation and cell size of Streptococcus mutans. Biofouling 2013; 29:307–318 [View Article][PubMed]
    [Google Scholar]
  14. Nostro A, Cannatelli MA, Crisafi G, Musolino AD, Procopio F et al. Modifications of hydrophobicity, in vitro adherence and cellular aggregation of Streptococcus mutans by Helichrysum italicum extract. Lett Appl Microbiol 2004; 38:423–427 [View Article][PubMed]
    [Google Scholar]
  15. Abbott A, Rutter PR, Berkeley RC. The influence of ionic strength, pH and a protein layer on the interaction between Streptococcus mutans and glass surfaces. J Gen Microbiol 1983; 129:439–445 [View Article][PubMed]
    [Google Scholar]
  16. Panagoda GJ, Ellepola AN, Samaranayake LP. Adhesion of Candida parapsilosis to epithelial and acrylic surfaces correlates with cell surface hydrophobicity. Mycoses 2001; 44:29–35 [View Article][PubMed]
    [Google Scholar]
  17. Sanguinetti M, Posteraro B, Lass-Flörl C. Antifungal drug resistance among Candida species: mechanisms and clinical impact. Mycoses 2015; 58:2–13 [View Article][PubMed]
    [Google Scholar]
  18. Graham HN, Composition G. Consumption, and polyphenol chemistry. Preventive Medicine 1992; 21:334–350 [CrossRef]
    [Google Scholar]
  19. Antunes DP, Salvia AC, de Araújo RM, di Nicoló R, Koga Ito CY et al. Effect of green tea extract and mouthwash without alcohol on Candida albicans biofilm on acrylic resin. Gerodontology 2015; 32:291–295 [View Article][PubMed]
    [Google Scholar]
  20. Betts JW, Wareham DW, Haswell SJ, Kelly SM. Antifungal synergy of theaflavin and epicatechin combinations against Candida albicans. J Microbiol Biotechnol 2013; 23:1322–1326 [View Article][PubMed]
    [Google Scholar]
  21. Chen M, Zhai L, Arendrup MC. In vitro activity of 23 tea extractions and epigallocatechin gallate against Candida species. Med Mycol 2015; 53:194–198 [View Article][PubMed]
    [Google Scholar]
  22. Doddanna SJ, Patel S, Sundarrao MA, Veerabhadrappa RS. Antimicrobial activity of plant extracts on Candida albicans: an in vitro study. Indian J Dent Res 2013; 24:401–405 [View Article][PubMed]
    [Google Scholar]
  23. Sitheeque MA, Panagoda GJ, Yau J, Amarakoon AM, Udagama UR et al. Antifungal activity of black tea polyphenols (catechins and theaflavins) against Candida species. Chemotherapy 2009; 55:189–196 [View Article][PubMed]
    [Google Scholar]
  24. Jenkinson HF, Douglas LJ. Interactions between Candida species and bacteria in mixed infections. In Brogden KA, Guthmiller JM. (editors) Polymicrobial Diseases Washington, DC: ASM Press; 2002 pp. 357–376 [CrossRef]
    [Google Scholar]
  25. Perva-Uzunalić A, Škerget M, Knez Ž, Weinreich B, Otto F et al. Extraction of active ingredients from green tea (Camellia sinensis): extraction efficiency of major catechins and caffeine. Food Chem 2006; 96:597–605 [View Article]
    [Google Scholar]
  26. James PA. Comparison of four methods for the determination of MIC and MBC of penicillin for viridans streptococci and the implications for penicillin tolerance. J Antimicrob Chemother 1990; 25:209–216 [View Article][PubMed]
    [Google Scholar]
  27. Wang Y, Lee SM, Dykes GA. Growth in the presence of sucrose may decrease attachment of some oral bacteria to abiotic surfaces. Ann Microbiol 2015; 65:1159–1163 [View Article]
    [Google Scholar]
  28. Barki M, Koltin Y, Yanko M, Tamarkin A, Rosenberg M. Isolation of a Candida albicans DNA sequence conferring adhesion and aggregation on Saccharomyces cerevisiae. J Bacteriol 1993; 175:5683–5689 [View Article][PubMed]
    [Google Scholar]
  29. Shen S, Samaranayake LP, Yip HK. Coaggregation profiles of the microflora from root surface caries lesions. Arch Oral Biol 2005; 50:23–32 [View Article][PubMed]
    [Google Scholar]
  30. Bandara HM, K Cheung BP, Watt RM, Jin LJ, Samaranayake LP. Pseudomonas aeruginosa lipopolysaccharide inhibits Candida albicans hyphae formation and alters gene expression during biofilm development. Mol Oral Microbiol 2013; 28:54–69 [View Article][PubMed]
    [Google Scholar]
  31. Jin Y, Zhang T, Samaranayake YH, Fang HH, Yip HK et al. The use of new probes and stains for improved assessment of cell viability and extracellular polymeric substances in Candida albicans biofilms. Mycopathologia 2005; 159:353–360 [View Article][PubMed]
    [Google Scholar]
  32. van Oss CJ, Good RJ, Chaudhury MK. The role of van der Waals forces and hydrogen bonds in “hydrophobic interactions” between biopolymers and low energy surfaces. J Colloid Interface Sci 1986; 111:378–390 [View Article]
    [Google Scholar]
  33. Alberti-Segui C, Morales AJ, Xing H, Kessler MM, Willins DA et al. Identification of potential cell-surface proteins in Candida albicans and investigation of the role of a putative cell-surface glycosidase in adhesion and virulence. Yeast 2004; 21:285–302 [View Article][PubMed]
    [Google Scholar]
  34. Wang Y, Lee SM, Dykes G. The physicochemical process of bacterial attachment to abiotic surfaces: challenges for mechanistic studies, predictability and the development of control strategies. Crit Rev Microbiol 2015; 41:452–464 [View Article][PubMed]
    [Google Scholar]
  35. Matsumoto M, Minami T, Sasaki H, Sobue S, Hamada S et al. Inhibitory effects of oolong tea extract on caries-inducing properties of mutans streptococci. Caries Res 1999; 33:441–445 [View Article][PubMed]
    [Google Scholar]
  36. Evensen NA, Braun PC. The effects of tea polyphenols on Candida albicans: inhibition of biofilm formation and proteasome inactivation. Can J Microbiol 2009; 55:1033–1039 [View Article][PubMed]
    [Google Scholar]
  37. Tan SM, Lee SM, Dykes GA. Acetic acid induces pH-independent cellular energy depletion in Salmonella enterica. Foodborne Pathog Dis 2015; 12:183–189 [View Article][PubMed]
    [Google Scholar]
  38. Teh AH, Wang Y, Dykes GA. The influence of antibiotic resistance gene carriage on biofilm formation by two Escherichia coli strains associated with urinary tract infections. Can J Microbiol 2014; 60:105–111 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.000555
Loading
/content/journal/jmm/10.1099/jmm.0.000555
Loading

Data & Media loading...

Most cited Most Cited RSS feed