1887

Abstract

Solid-organ transplant recipients may display high rates of colonization and/or infection by multidrug-resistant bacteria. We analysed and compared the phenotypic and genotypic diversity of carbapenem-resistant (CR) strains of , and isolated from patients in the Solid Organ Transplantation department of our hospital.

Between March 2012 and August 2013, 56 CR strains from various biological fluids underwent antimicrobial susceptibility testing with VITEK 2, molecular analysis by PCR amplification and genotypic analysis with pulsed-field gel electrophoresis (PFGE). They were clustered according to antimicrobial drug susceptibility and genotypic profiles. Diversity analyses were performed by calculating Simpson’s diversity index and applying computed rarefaction curves.

Among , KP-producers predominated (57.1 %). VIM and OXA-23 carbapenemases prevailed among and (89.4 and 88.9 %, respectively). KPC-producing and OXA-23 were assigned in single PFGE pulsotypes. VIM-producing generated multiple pulsotypes. CR strains displayed phenotypic diversity in tigecycline, colistin (CS), amikacin (AMK), gentamicin (GEN) and co-trimoxazole (SXT) (16 clusters); displayed phenotypic diversity in cefepime (FEP), ceftazidime, aztreonam, piperacillin, piperacillin–tazobactam, AMK, GEN and CS (9 clusters); and displayed phenotypic diversity in AMK, GEN, SXT, FEP, tobramycin and rifampicin (8 clusters). The Simpson diversity indices for the interpretative phenotype and PFGE analysis were 0.89 and 0.6, respectively, for strains (<0.001); 0.77 and 0.6 for (=0.22); and 0.86 and 0.19 for (=0.004).

The presence of different antimicrobial susceptibility profiles does not preclude the possibility that two CR or isolates are clonally related.

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2017-08-01
2024-03-29
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