1887

Abstract

Background. Sustainable suppression of HIV replication forms the basis of anti-retroviral therapy (ART) medication. Thus, reliable quantification of HIV viral load has become an essential factor to monitor the effectiveness of the ART. Longer turnaround-time (TAT), batch testing and technical skills are major drawbacks of standard real-time PCR assays.

Methods. The performance of the point-of-care Xpert HIV-1 viral load assay was evaluated against the Abbott RealTime PCR m2000rt system. A total of 96 plasma specimens ranging from 2.5 log10 copies ml to 4.99 log10 copies ml and proficiency testing panel specimens were used. Precision and accuracy were checked using the Pearson correlation co-efficient test and Bland–Altman analysis.

Results. Compared to the Abbott RealTime PCR, the Xpert HIV-1 viral load assay showed a good correlation (Pearson r=0.81; P<0.0001) with a mean difference of 0.27 log10 copies ml (95 % CI, −0.41 to 0.96 log10 copies ml; sd, 0.35 log10 copies ml).

Conclusion. Reliable and ease of testing individual specimens could make the Xpert HIV-1 viral load assay an efficient alternative method for ART monitoring in clinical management of HIV disease in resource-limited settings. The rapid test results (less than 2 h) could help in making an immediate clinical decision, which further strengthens patient care.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000514
2017-09-13
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/jmm/66/10/1379.html?itemId=/content/journal/jmm/10.1099/jmm.0.000514&mimeType=html&fmt=ahah

References

  1. Balakrishnan P, Kumarasamy N, Kantor R, Solomon S, Vidya S et al. HIV type 1 genotypic variation in an antiretroviral treatment-naive population in southern India. AIDS Res Hum Retroviruses 2005;21:301–305 [CrossRef][PubMed]
    [Google Scholar]
  2. Saravanan S, Vidya M, Balakrishnan P, Kantor R, Solomon SS et al. Viremia and HIV-1 drug resistance mutations among patients receiving second-line highly active antiretroviral therapy in Chennai, Southern India. Clin Infect Dis 2012;54:995–1000 [CrossRef][PubMed]
    [Google Scholar]
  3. WHO Consolidated guidelines on the use of antiretroviral drugs for treating and preventing HIV infection [Internet]. Available fromwww.who.int/hiv/pub/arv/arv-2016/en/ [cited 2016 Jul 8]
  4. Sollis KA, Smit PW, Fiscus S, Ford N, Vitoria M et al. Systematic review of the performance of HIV viral load technologies on plasma samples. PLoS One 2014;9:e85869 [CrossRef][PubMed]
    [Google Scholar]
  5. Reynolds SJ, Nakigozi G, Newell K, Ndyanabo A, Galiwongo R et al. Failure of immunologic criteria to appropriately identify antiretroviral treatment failure in Uganda. AIDS 2009;23:697–700 [CrossRef][PubMed]
    [Google Scholar]
  6. Jordan JA, Plantier JC, Templeton K, Wu AH. Multi-site clinical evaluation of the Xpert® HIV-1 viral load assay. J Clin Virol 2016;80:27–32 [CrossRef][PubMed]
    [Google Scholar]
  7. Mor O, Gozlan Y, Wax M, Mileguir F, Rakovsky A et al. Evaluation of the realtime HIV-1, Xpert HIV-1, and aptima HIV-1 quant Dx assays in comparison to the NucliSens easyQ HIV-1 v2.0 assay for quantification of HIV-1 viral load. J Clin Microbiol 2015;53:3458–3465 [CrossRef][PubMed]
    [Google Scholar]
  8. Doyle T, Smith C, Vitiello P, Cambiano V, Johnson M et al. Plasma HIV-1 RNA detection below 50 copies/mL and risk of virologic rebound in patients receiving highly active antiretroviral therapy. Clin Infect Dis 2012;54:724–732 [CrossRef][PubMed]
    [Google Scholar]
  9. Gueudin M, Baron A, Alessandri-Gradt E, Lemée V, Mourez T et al. Performance evaluation of the new HIV-1 quantification assay, Xpert HIV-1 viral load, on a wide panel of HIV-1 variants. J Acquir Immune Defic Syndr 2016;72:521–526 [CrossRef][PubMed]
    [Google Scholar]
  10. Ceffa S, Luhanga R, Andreotti M, Brambilla D, Erba F et al. Comparison of the Cepheid GeneXpert and Abbott M2000 HIV-1 real time molecular assays for monitoring HIV-1 viral load and detecting HIV-1 infection. J Virol Methods 2016;229:35–39 [CrossRef][PubMed]
    [Google Scholar]
  11. Moyo S, Mohammed T, Wirth KE, Prague M, Bennett K et al. Point-of-care cepheid Xpert HIV-1 viral load test in rural African communities is feasible and reliable. J Clin Microbiol 2016;54:3050–3055 [CrossRef][PubMed]
    [Google Scholar]
  12. Garrett NJ, Drain PK, Werner L, Samsunder N, Abdool Karim SS. Diagnostic accuracy of the point-of-care Xpert HIV-1 viral load assay in a South African HIV Clinic. J Acquir Immune Defic Syndr 2016;72:e45e48 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.000514
Loading
/content/journal/jmm/10.1099/jmm.0.000514
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error