1887

Abstract

Purpose. The increasing number of infections caused by nontuberculous mycobacteria (NTM) has prompted the need for rapid and precise identification methods of these pathogens. Several studies report the applicability of MALDI-TOF mass spectrometry (MS) for identification of NTM. However, some closely related species have very similar spectral mass fingerprints, and until recently, Mycobacterium chimaera and M. intracellulare could not be separated from each other by MALDI-TOF MS.

Methodology. The conventional identification methods used in routine diagnostics have similar limitations. Recently, the differentiation of these two species within the Mycobacterium avium complex has become increasingly important due to reports of M. chimaera infections related to open heart surgery in Europe and in the USA. In this report, a method for the distinct differentiation of M. chimaera and M. intracellulare using a more detailed analysis of MALDI-TOF mass spectra is presented.

Key Findings. Species-specific peaks could be identified and it was possible to assign all isolates (100 %) from reference strain collections as well as clinical isolates to the correct species.

Conclusions. We have developed a model for the accurate identification of M. chimaera and M. intracellulare by MALDI-TOF MS. This approach has the potential for routine use in microbiology laboratories, as the model itself can be easily implemented into the software of the currently available systems by MALDI-TOF MS manufacturers.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000469
2017-05-15
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/jmm/66/5/670.html?itemId=/content/journal/jmm/10.1099/jmm.0.000469&mimeType=html&fmt=ahah

References

  1. Griffith DE, Aksamit T, Brown-Elliott BA, Catanzaro A, Daley C et al. An official ATS/IDSA statement: diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases. Am J Respir Crit Care Med 2007;175:367–416 [CrossRef][PubMed]
    [Google Scholar]
  2. Winthrop KL, McNelley E, Kendall B, Marshall-Olson A, Morris C et al. Pulmonary nontuberculous mycobacterial disease prevalence and clinical features: an emerging public health disease. Am J Respir Crit Care Med 2010;182:977–982 [CrossRef][PubMed]
    [Google Scholar]
  3. Cuttino JT, McCabe AM. Pure granulomatous nocardiosis: a new fungus disease distinguished by intracellular parasitism. Am J Pathol 1949;25:1–47
    [Google Scholar]
  4. Tortoli E, Rindi L, Garcia MJ, Chiaradonna P, Dei R et al. Proposal to elevate the genetic variant MAC-A, included in the Mycobacterium avium complex, to species rank as Mycobacterium chimaera sp. nov. Int J Syst Evol Microbiol 2004;54:1277–1285 [CrossRef][PubMed]
    [Google Scholar]
  5. Schweickert B, Goldenberg O, Richter E, Göbel UB, Petrich A et al. Occurrence and clinical relevance of Mycobacterium chimaera sp. nov., Germany. Emerg Infect Dis 2008;14:1443–1446 [CrossRef][PubMed]
    [Google Scholar]
  6. Wallace RJ, Iakhiaeva E, Williams MD, Brown-Elliott BA, Vasireddy S et al. Absence of Mycobacterium intracellulare and presence of Mycobacterium chimaera in household water and biofilm samples of patients in the United States with Mycobacterium avium complex respiratory disease. J Clin Microbiol 2013;51:1747–1752 [CrossRef][PubMed]
    [Google Scholar]
  7. Makovcova J, Slany M, Babak V, Slana I, Kralik P. The water environment as a source of potentially pathogenic mycobacteria. J Water Health 2014;12:254–263 [CrossRef][PubMed]
    [Google Scholar]
  8. Boyle DP, Zembower TR, Reddy S, Qi C. Comparison of clinical features, virulence, and relapse among Mycobacterium avium complex species. Am J Respir Crit Care Med 2015;191:1310–1317 [CrossRef][PubMed]
    [Google Scholar]
  9. Centers for Disease Control and Prevention Non-Tuberculous Mycobacterium (NTM) Infections and Heater-Cooler Devices Interim Practical Guidance Atlanta, GA: Centers for Disease Control and Prevention; 2015;www.cdc.gov/hai/pdfs/outbreaks/CDC-Notice-Heater-Cooler-Units-final-clean.pdf
    [Google Scholar]
  10. European Centre for Disease Prevention and Control Invasive Cardiovascular Infection by Mycobacterium Chimaera Potentially Associated with Heater-Cooler-Units Used During Cardiac Surgery Solna, Sweden: European Centre for Disease Prevention and Control; 2015;http://ecdc.europa.eu/en/publications/Publications/mycobacterium-chimaera-infection-associated-with-heater-cooler-units-rapid-risk-assessment-30-April-2015.pdf
    [Google Scholar]
  11. Sax H, Bloemberg G, Hasse B, Sommerstein R, Kohler P et al. Prolonged outbreak of Mycobacterium chimaera infection after open-chest heart surgery. Clin Infect Dis 2015;61:67–75 [CrossRef][PubMed]
    [Google Scholar]
  12. Sommerstein R, Rüegg C, Kohler P, Bloemberg G, Kuster SP et al. Transmission of Mycobacterium chimaera from heater-cooler units during cardiac surgery despite an ultraclean air ventilation system. Emerg Infect Dis 2016;22:1008–1013 [CrossRef][PubMed]
    [Google Scholar]
  13. Russo C, Tortoli E, Menichella D. Evaluation of the new Geno Type Mycobacterium assay for identification of mycobacterial species. J Clin Microbiol 2006;44:334–339[CrossRef]
    [Google Scholar]
  14. Tortoli E, Pecorari M, Fabio G, Messinò M, Fabio A. Commercial DNA probes for mycobacteria incorrectly identify a number of less frequently encountered species. J Clin Microbiol 2010;48:307–310 [CrossRef][PubMed]
    [Google Scholar]
  15. Bruker Daltonik Release Notes–MBT Mycobacteria Library (880 MSPs) Bremen: Bruker Daltonik; 2016
    [Google Scholar]
  16. Boyle DP, Zembower TR, Qi C. Evaluation of Vitek MS for rapid classification of clinical isolates belonging to Mycobacterium avium complex. Diagn Microbiol Infect Dis 2015;81:41–43 [CrossRef][PubMed]
    [Google Scholar]
  17. Saleeb PG, Drake SK, Murray PR, Zelazny AM. Identification of mycobacteria in solid-culture media by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 2011;49:1790–1794 [CrossRef][PubMed]
    [Google Scholar]
  18. Jung JS, Popp C, Sparbier K, Lange C, Kostrzewa M et al. Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for rapid detection of β-lactam resistance in Enterobacteriaceae derived from blood cultures. J Clin Microbiol 2014;52:924–930 [CrossRef][PubMed]
    [Google Scholar]
  19. Griffith DE, Brown-Elliott BA, Wallace RJ. Hit the road, MAC, and don't you come back no more. Am J Respir Crit Care Med 2015;191:1222–1224 [CrossRef][PubMed]
    [Google Scholar]
  20. Balada-Llasat JM, Kamboj K, Pancholi P. Identification of mycobacteria from solid and liquid media by matrix-assisted laser desorption ionization-time of flight mass spectrometry in the clinical laboratory. J Clin Microbiol 2013;51:2875–2879 [CrossRef][PubMed]
    [Google Scholar]
  21. Rodríguez-Sánchez B, Ruiz-Serrano MJ, Marín M, López Roa P, Rodríguez-Créixems M et al. Evaluation of matrix-assisted laser desorption ionization–time of flight mass spectrometry for identification of nontuberculous mycobacteria from clinical isolates. J Clin Microbiol 2015;53:2737–2740 [CrossRef][PubMed]
    [Google Scholar]
  22. Kehrmann J, Schoerding AK, Murali R, Wessel S, Koehling HL et al. Performance of Vitek MS in identifying nontuberculous mycobacteria from MGIT liquid medium and Lowenstein-Jensen solid medium. Diagn Microbiol Infect Dis 2016;84:43–47 [CrossRef][PubMed]
    [Google Scholar]
  23. Rodríguez-Temporal D, Pérez-Risco D, Struzka EA, Mas M, Rodríguez D et al. Differentiation of closely related mycobacterial species from Mycobacterium intracellulare and Mycobacterium fortuitum complex by mass spectrometry (MALDI-TOF). Eur J Clin Microbiol Infect Dis 2016
    [Google Scholar]
  24. Teng SH, Chen CM, Lee MR, Lee TF, Chien KY et al. Matrix-assisted laser desorption ionization-time of flight mass spectrometry can accurately differentiate between Mycobacterium masilliense (M. abscessus subspecies bolletti) and M. abscessus (sensu stricto). J Clin Microbiol 2013;51:3113–3116 [CrossRef][PubMed]
    [Google Scholar]
  25. Fangous MS, Mougari F, Gouriou S, Calvez E, Raskine L et al. Classification algorithm for subspecies identification within the Mycobacterium abscessus species, based on matrix-assisted laser desorption ionization–time of flight mass spectrometry. J Clin Microbiol 2014;52:3362–3369 [CrossRef][PubMed]
    [Google Scholar]
  26. Suzuki H, Yoshida S, Yoshida A, Okuzumi K, Fukusima A et al. A novel cluster of Mycobacterium abscessus complex revealed by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Diagn Microbiol Infect Dis 2015;83:365–370 [CrossRef][PubMed]
    [Google Scholar]
  27. Panagea T, Pincus DH, Grogono D, Jones M, Bryant J et al. Mycobacterium abscessus complex identification with matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 2015;53:2355–2358 [CrossRef][PubMed]
    [Google Scholar]
  28. Kehrmann J, Wessel S, Murali R, Hampel A, Bange FC et al. Principal component analysis of MALDI TOF MS mass spectra separates M. abscessus (sensu stricto) from M. massiliense isolates. BMC Microbiol 2016;16:24 [CrossRef][PubMed]
    [Google Scholar]
  29. Pranada AB, Timke M, Witt E, Kostrzewa M. Looking at the differences: first report of possibilities to separate Mycobacterium chimaera from M. intracellulare by MALDI-TOF MS. Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC/ICC), September 17 21, 2015, San Diego, CA, USA; Poster D-223www.abstractsonline.com/Plan/ViewAbstract.aspx?sKey=92d06ecf-43c8-45a9-8e94-6fee93149c66&cKey=c573521e-a3ff-4530-b0f9-2955427ad167&mKey=%7b7A574A80-EAB1-4B50-B343-4695DF14907E%7d
  30. Månsson V, Resman F, Kostrzewa M, Nilson B, Riesbeck K. Identification of Haemophilus influenzae type b isolates by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 2015;53:2215–2224 [CrossRef][PubMed]
    [Google Scholar]
  31. van Ingen J, Hoefsloot W, Buijtels PC, Tortoli E, Supply P et al. Characterization of a novel variant of Mycobacterium chimaera. J Med Microbiol 2012;61:1234–1239 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.000469
Loading
/content/journal/jmm/10.1099/jmm.0.000469
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error