RT Journal Article SR Electronic(1) A1 Oliveira-Pinto, Clarisse A1 Diamantino, Cristiane A1 Oliveira, Patrícia L A1 Reis, Mariana P A1 Costa, Patrícia S A1 Paiva, Magna C A1 Nardi, Regina M. D A1 Magalhães, Paula P A1 Chartone-Souza, Edmar A1 Nascimento, Andréa M. AYR 2017 T1 Occurrence and characterization of class 1 integrons in Escherichia coli from healthy individuals and those with urinary infection JF Journal of Medical Microbiology, VO 66 IS 5 SP 577 OP 583 DO https://doi.org/10.1099/jmm.0.000468 PB Microbiology Society, SN 1473-5644, AB Purpose. Class 1 integrons are among the main vehicles that facilitate the spread of antibiotic-resistance genes, with serious public health consequences. The aim of this cross-sectional study was to investigate the presence of class 1 integrons and to characterize their variable regions, as well as the antimicrobial resistance profiles and phylogenetic groups of a collection of Escherichia coli isolates recovered from healthy subjects (n=42) and those with urinary infection (n=40). Methodology. The methods used included PCR, sequencing and antimicrobial susceptibility testing. Results. PCR screening for the integrase gene (intI1) revealed a higher incidence of class 1 integrons in uropathogenic E. coli (65 %, UPEC) than in commensal isolates (11.9 %). Eight of 31 intI1-positive isolates, all of them UPEC, harboured empty integrons. The variable regions of the other 23 contained gene cassettes encoding resistance to β-lactams (blaOXA-1), aminoglycosides (aadA1 and aadA5), trimethoprim (dfrA1 and dfrA17) and an ORF. To our knowledge this is the first report of an ORF identified as a putative phage tail protein associated with a class 1 integron. The aadA1 and dfrA17-addA5 arrays prevailed in commensal E. coli and UPEC, respectively. UPEC isolates were highly resistant to the antimicrobials tested, in contrast to commensal isolates. The E. coli isolates carrying gene cassettes associated with class 1 integrons were found to be unrelated to any phylogroup or multiresistance. Conclusion. Co-resistance to clinically relevant fluoroquinolone and trimethoprim-sulfamethazole in all UPEC isolates is a cause for concern. These results expand the current knowledge of gene cassettes in both commensal and pathogenic E. coli., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000468