1887

Abstract

is a Thai medicinal plant that has been attracting attention for its remarkable antibacterial properties against Gram-positive pathogenic bacteria. The purpose of this study was to evaluate the antibacterial properties of leaf extract against and isolated from infected tilapia.

The anti-streptococcal activity of was determined using broth microdilution assays.

The extract demonstrated strong antibacterial activity against the fish pathogens, with minimum inhibitory concentrations (MICs) ranging from 7.8‒62.5 µg ml. It was found to possess a dose-dependent bacteriostatic effect on this organism. Scanning electron microscopy revealed irregular and long chains of swollen cells, as well as corkscrew shapes andincomplete separation of cell division of cells following the treatment at sub-MIC. Moreover, cells pre-treated with the extract became more sensitive to oxidative stress induced by HO than the untreated cells. Based on the mortality of Nile tilapia after intraperitoneal infection of at median lethal dose (LD), the pre-treated cells caused a significant (<0.01) reduction in mortality of -infected Nile tilapia.

The results suggested that could be further developed as a simple and effective agent for the treatment of streptococcosis in Nile tilapia.

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2017-04-01
2024-12-12
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