%0 Journal Article %A Lima, Kledoaldo %A Leal, Élcio %A Cavalcanti, Ana Maria Salustiano %A Salustiano, Daniela Medeiros %A de Medeiros, Luzidalva Barbosa %A da Silva, Sirleide Pereira %A Lacerda, Heloísa Ramos %T Increase in human immunodeficiency virus 1 diversity and detection of various subtypes and recombinants in north-eastern Brazil %D 2017 %J Journal of Medical Microbiology, %V 66 %N 4 %P 526-535 %@ 1473-5644 %R https://doi.org/10.1099/jmm.0.000447 %K genetic diversity %K subtypes %K pol gene %K molecular epidemiology %K phylogenetic analyses %K HIV-1 %I Microbiology Society, %X Purpose. Diverse human immunodeficiency virus 1 (HIV-1) subtypes and circulating recombinant forms are found in Brazil. The majority of HIV-1 molecular epidemiological studies in Brazil have been conducted in the southern and south-eastern regions of the country, although several recent studies in the north-eastern region have addressed this issue. The objective of this study was to molecularly characterize HIV-1 circulating in Pernambuco, north-eastern Brazil. Methodology. A total of 64 samples were collected from 2002 to 2003, and another 103 were collected from 2007 to 2009. The protease and partial reverse transcriptase regions of the HIV-1 polymerase-encoding (pol) gene were sequenced, and subtyping, recombination and phylogenetic analyses were performed. Results/Key findings. Subtype B (60.9 %) was found to be predominant, followed by HIV-1 F (31.4 %). Several BF recombinants (4.2 %), and BC and AG recombinants were also identified. The intra-subtype genetic diversity was estimated to be 0.065 (sd±0.004) for HIV-1 B and 0.055 (sd±0.004) for HIV-1 F, reflecting a greater accumulation of mutations in subtype B (P<0.01). More codons were found to be under positive selective pressure in samples collected from 2007 to 2009, from individuals with a T-cell count≥200 cells mm−3 and from women. Coalescence data indicated that the subtype F population has been continuously expanding. Conclusions. HIV-1 shows high genetic diversity in the state of Pernambuco. Thus, additional molecular evaluations of circulating strains will provide a better understanding of the epidemic and may lead to more effective preventive strategies. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000447