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Volume 66, Issue 4

Increase in human immunodeficiency virus 1 diversity and detection of various subtypes and recombinants in north-eastern Brazil Free

Abstract

Diverse human immunodeficiency virus 1 (HIV-1) subtypes and circulating recombinant forms are found in Brazil. The majority of HIV-1 molecular epidemiological studies in Brazil have been conducted in the southern and south-eastern regions of the country, although several recent studies in the north-eastern region have addressed this issue. The objective of this study was to molecularly characterize HIV-1 circulating in Pernambuco, north-eastern Brazil.

A total of 64 samples were collected from 2002 to 2003, and another 103 were collected from 2007 to 2009. The protease and partial reverse transcriptase regions of the HIV-1 polymerase-encoding () gene were sequenced, and subtyping, recombination and phylogenetic analyses were performed.

Subtype B (60.9 %) was found to be predominant, followed by HIV-1 F (31.4 %). Several BF recombinants (4.2 %), and BC and AG recombinants were also identified. The intra-subtype genetic diversity was estimated to be 0.065 (±0.004) for HIV-1 B and 0.055 (±0.004) for HIV-1 F, reflecting a greater accumulation of mutations in subtype B (<0.01). More codons were found to be under positive selective pressure in samples collected from 2007 to 2009, from individuals with a T-cell count≥200 cells mm and from women. Coalescence data indicated that the subtype F population has been continuously expanding.

HIV-1 shows high genetic diversity in the state of Pernambuco. Thus, additional molecular evaluations of circulating strains will provide a better understanding of the epidemic and may lead to more effective preventive strategies.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): genetic diversity , HIV-1 , molecular epidemiology , phylogenetic analyses , pol gene and subtypes
© 2017 The Authors
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2017-04-01
2024-04-20
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Increase in human immunodeficiency virus 1 diversity and detection of various subtypes and recombinants in north-eastern Brazil
J. Med. Microbiol. 66, 526 (2017); https://doi.org/10.1099/jmm.0.000447
/content/journal/jmm/10.1099/jmm.0.000447
/content/journal/jmm/10.1099/jmm.0.000447
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