@article{mbs:/content/journal/jmm/10.1099/jmm.0.000416, author = "Legione, Alistair R and Patterson, Jade L. S and Whiteley, Pam and Firestone, Simon M and Curnick, Megan and Bodley, Kate and Lynch, Michael and Gilkerson, James R and Sansom, Fiona M and Devlin, Joanne M", title = "Koala retrovirus genotyping analyses reveal a low prevalence of KoRV-A in Victorian koalas and an association with clinical disease", journal= "Journal of Medical Microbiology", year = "2017", volume = "66", number = "2", pages = "236-244", doi = "https://doi.org/10.1099/jmm.0.000416", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000416", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", keywords = "KoRV-B", keywords = "prevalence", keywords = "KoRV-A", keywords = "koala retrovirus", keywords = "Victoria", keywords = "genotype", abstract = " Purpose. Koala retrovirus (KoRV) is undergoing endogenization into the genome of koalas in Australia, providing an opportunity to assess the effect of retrovirus infection on the health of a population. The prevalence of KoRV in north-eastern Australia (Queensland and New South Wales) is 100 %, whereas previous preliminary investigations in south-eastern Australia (Victoria) suggested KoRV is present at a lower prevalence, although the values have varied widely. Here, we describe a large study of free-ranging koalas in Victoria to estimate the prevalence of KoRV and assess the clinical significance of KoRV infection in wild koalas. Methodology. Blood or spleen samples from 648 koalas where tested for KoRV provirus, and subsequently genotyped, using PCRs to detect the pol and env genes respectively. Clinical data was also recorded where possible and analysed in comparison to infection status. Results. The prevalence of KoRV was 24.7 % (160/648). KoRV-A was detected in 141/160 cases, but KoRV-B, a genotype associated with neoplasia in captive koalas, was not detected. The genotype in 19 cases could not be determined. Genomic differences between KoRV in Victoria and type strains may have impacted genotyping. Factors associated with KoRV infection, based on multivariable analysis, were low body condition score, region sampled, and ‘wet bottom’ (a staining of the fur around the rump associated with chronic urinary incontinence). Koalas with wet bottom were nearly twice as likely to have KoRV provirus detected than those without wet bottom (odds ratio=1.90, 95 % confidence interval 1.21, 2.98). Conclusion. Our findings have important implications for the conservation of this iconic species, particularly regarding translocation potential of Victorian koalas.", }