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Volume 66, Issue 2

Determination of antimicrobial resistance of strains isolated from pigs and their genotypic characterization by method of amplification of DNA fragments surrounding rare restriction sites (ADSRRS fingerprinting) Free

Abstract

In this study, we analysed phenotypic resistance profiles and their reflection in the genomic profiles of spp. strains isolated from pigs raised on different farms.

Samples were collected from five pig farms (=90 animals) and tested for . MICs of 12 antimicrobials were determined using the broth microdilution method, and epidemiological molecular analysis of strains belonging to selected species (, and ) was performed using the ADSRRS-fingerprinting (amplification of DNA fragments surrounding rare restriction sites) method with a few modifications.

The highest percentage of strains was resistant to tetracycline (73.4 %), erythromycin and tylosin (42.5 %) and rifampin (25.2 %), and a large number of strains exhibited high-level resistance to both kanamycin (25.2 %) and streptomycin (27.6 %). The strains of , and (=184) revealed varied phenotypic resistance profiles, among which as many as seven met the criteria for multidrug resistance (30.4 % of strains tested). ADSRRS-fingerprinting analysis produced 17 genotypic profiles of individual strains which were correlated with their phenotypic resistance profiles. Only strains susceptible to all of the chemotherapeutics tested had two different ADSRRS profiles. Moreover, eight animals were carriers of more than one genotype belonging to the same spp., mainly .

Given the possibility of transmission to humans of the high-resistance/multidrug resistance enterococci and the significant role of pigs as food animals in this process, it is necessary to introduce a multilevel control strategy by carrying out research on the resistance and molecular characteristics of indicator bacterial strains isolated from animals on individual farms.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): ADSRRS-fingerprinting , antibiotic resistance , Enterococcus , multi-drug resistance and pig farms
© 2017 The Authors
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2017-02-01
2024-04-16
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Determination of antimicrobial resistance of Enterococcus strains isolated from pigs and their genotypic characterization by method of amplification of DNA fragments surrounding rare restriction sites (ADSRRS fingerprinting)
J. Med. Microbiol. 66, 175 (2017); https://doi.org/10.1099/jmm.0.000400
/content/journal/jmm/10.1099/jmm.0.000400
/content/journal/jmm/10.1099/jmm.0.000400
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