1887

Abstract

The objective of the present study was to evaluate the prevalence of intestinal colonization with extended-spectrum β-lactamase (ESBL)-producing (ESBL-E) and carbapenemase-producing (CPE) in non-selected hospitalized and non-hospitalized patients from the same geographic area of Madrid.

A total of 501 fecal samples were screened. Diluted samples in saline were cultured in MacConkey agar plates with ceftazidime, cefotaxime, imipenem and meropenem disks. Colonies growing within the inhibition zone of either disk were selected. Characterization of ESBLs and CPEs were performed by PCR and sequencing. The Wider system was used for the bacterial identification. In addition, clonal analysis was carried out for species predominant among the fecal carriage.

Among the 501 patients enrolled, 43 (8.6 %) carried ESBL-E and 8 (1.6 %) patients exhibited CPE. The main intestinal colonizer among ESBL-E was CTX-M-producing isolates in both settings (community and hospital). ST131 clonal complex was the most common among faecal ESBL-producing . All gut carriers of CPE were hospitalized patients, being the most prevalent species. Two OXA-48-producing isolates belonging to ST15 were detected.

Present study reveals that faecal carriage of ESBL is common among inpatients and outpatients, whereas carbapenemase producers are only present in the hospital setting. Therefore, active surveillance will be useful for reducing transmission of antimicrobial-resistant bacteria and preventing infection.

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2017-02-01
2019-12-15
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