1887

Abstract

and complex are the two species of this genus most involved in healthcare-associated infections that are ESBL and carbapenemase producers. This study characterized, phenotypically and genotypically, 51 isolates of and complex originating from infection or colonization in patients admitted to a public hospital in Recife, Pernambuco, Brazil, by antimicrobial susceptibility profile, analysis of β-lactamase genes ( , , , , , and ), PCR and DNA sequencing, plasmid profile and ERIC-PCR. In both species, the genes , and were detected. The DNA sequencing confirmed the variants , and in isolates. More than one gene conferring resistance in the isolates, including the detection of the three previously cited genes in strains isolated from infection sites, was observed. The detection of was more frequent in isolates from infection sites than from colonization. The gene predominated in complex isolates obtained from infections; however, in isolates, it predominated in samples obtained from colonization. A clonal relationship among all of isolates was detected by ERIC-PCR. The majority of complex isolates presented the same ERIC-PCR pattern. Despite the clonal relation presented by the isolates using ERIC-PCR, different plasmid and resistance profiles and several resistance genes were observed. The clonal dissemination and the accumulation of β-lactam resistance determinants presented by the isolates demonstrated the ability of and complex, obtained from colonization and infection, to acquire and maintain different resistance genes.

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2017-01-01
2024-03-28
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