1887

Abstract

is an anaerobic bacterium that causes deep infection in organs and prosthetic joints, in addition to acne vulgaris. Many tetracycline-resistant strains have been isolated because oral tetracyclines are frequently used as an acne treatment against . In this study, we found a novel tetracycline resistance mechanism in . Three doxycycline-resistant (MIC: 16 µg ml) strains were isolated from 69 strains in acne patients in Japan between 2010 and 2011. Additionally, six insusceptible strains (MIC: 1–2 µg ml) that had reduced susceptibility compared to susceptible strains (MIC: ≤0.5 µg ml) were identified. All doxycycline-resistant strains had a G1036C mutation in the 16S rRNA gene in addition to an amino acid substitution in the ribosomal S10 protein encoded by . By contrast, insusceptible strains had an amino acid substitution in the S10 protein but no mutation in the 16S rRNA. When the mutant with decreased susceptibility to doxycycline was obtained , only the mutated S10 protein was found (MIC: 4 µg ml), not the mutated 16S rRNA gene. This result shows that the S10 protein amino acid substitution contributes to reduced doxycycline susceptibility in and suggests that tetracycline resistance is acquired through a 16S rRNA mutation after the S10 protein amino acid substitution causes reduced susceptibility.

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2017-01-01
2020-03-31
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