@article{mbs:/content/journal/jmm/10.1099/jmm.0.000391, author = "Ou, Qin and Fan, Jinming and Duan, Dejian and Xu, Li and Wang, Jie and Zhou, Dongsheng and Yang, Huiying and Li, Bei", title = "Involvement of cAMP receptor protein in biofilm formation, fimbria production, capsular polysaccharide biosynthesis and lethality in mouse of Klebsiella pneumoniae serotype K1 causing pyogenic liver abscess", journal= "Journal of Medical Microbiology", year = "2017", volume = "66", number = "1", pages = "1-7", doi = "https://doi.org/10.1099/jmm.0.000391", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000391", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", keywords = "capsular polysaccharide", keywords = "Klebsiella pneumoniae", keywords = "CRP", keywords = "biofilm formation", keywords = "virulence", abstract = "The global regulator cAMP receptor protein (CRP) has been shown to be required for the full virulence and/or for the expression of virulence determinants in a wide set of bacterial pathogens. In this work, the crp mutant as well as the complemented mutant was constructed from a wild-type Klebsiella pneumoniae capsular serotype K1 strain causing the primary pyogenic liver abscess. The phenotypes of wild-type strain, crp mutant and complemented mutant were characterized systematically. It was disclosed that K. pneumoniae CRP was required for the in vitro growth, fimbria production, biofilm formation and lethality in mouse, but it inhibited the capsular polysaccharide biosynthesis. These indicated the important roles of CRP in regulating the expression of virulence and biofilm genes in K. pneumoniae.", }