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Volume 65, Issue 12

Multiple-locus variable-number tandem repeat fingerprinting as a method for rapid and cost-effective typing of animal-associated strains from lineages other than sequence type 398 Free

Abstract

In veterinary medicine, is associated with a range of mild to severe infections. The high density of livestock in intensive farming systems increases the risk of disease spread and hampers its control and measures of prevention, making one of the most important animal pathogens. Multiple-locus variable-number tandem repeat fingerprinting (MLVF) has been successfully applied to the characterization of livestock-associated meticillin-resistant (MRSA) ST398 but not to the characterization of a wide range of other animal isolates. The objective of the current study was to examine the effectiveness of MLVF for studying strains isolated from households, farms and exotic animals in three regions of Poland. MLVF, random amplification of polymorphic DNA (RAPD), typing and diagnostic microarrays were compared to determine the most suitable combination of methods for veterinary purposes. MLVF generated results consistent with host and geographic origins, reflecting population structures with a high concordance to typing results. MLVF has been proven to be a rapid, highly discriminatory and cost-effective method suitable for molecular typing in veterinary settings.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): animal-associated staphylococci , microarray , MLVF , spa typing , Staphylococcus aureus and veterinary
© 2016 The Authors
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/content/journal/jmm/10.1099/jmm.0.000378
2016-12-16
2024-04-25
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Multiple-locus variable-number tandem repeat fingerprinting as a method for rapid and cost-effective typing of animal-associated Staphylococcus aureus strains from lineages other than sequence type 398
J. Med. Microbiol. 65, 1494 (2016); https://doi.org/10.1099/jmm.0.000378
/content/journal/jmm/10.1099/jmm.0.000378
/content/journal/jmm/10.1099/jmm.0.000378
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