@article{mbs:/content/journal/jmm/10.1099/jmm.0.000377, author = "Palma, Thaís H. and Harth-Chú, Erika N. and Scott, Jodie and Stipp, Rafael N. and Boisvert, Heike and Salomão, Mariana F. and Theobaldo, Jéssica D. and Possobon, Rosana F. and Nascimento, Leandro C. and McCafferty, Jonathan W. and Faller, Lina and Duncan, Margaret J. and Mattos-Graner, Renata O.", title = "Oral cavities of healthy infants harbour high proportions of Streptococcus salivarius strains with phenotypic and genotypic resistance to multiple classes of antibiotics", journal= "Journal of Medical Microbiology", year = "2016", volume = "65", number = "12", pages = "1456-1464", doi = "https://doi.org/10.1099/jmm.0.000377", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000377", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", keywords = "children.", keywords = "oral microbiota", keywords = "Streptococcus salivarius", keywords = "oral colonization", keywords = "antibiotic resistance genes", keywords = "antibiotic resistance", abstract = "Emerging antibiotic resistance in the oropharyngeal microbiota, of which Streptococcus salivarius is a prominent species, represents a challenge for treating paediatric populations. In this study, we investigated the role of Streptococcus salivarius as a reservoir for antibiotic resistance genes (ARG) in the oral microbiota by analysing 95 Streptococcus salivarius isolates from 22 healthy infants (2–16 months of age). MICs of penicillin G, amoxicillin, erythromycin, tetracycline, doxycycline and streptomycin were determined. ARG profiles were assessed in a subset of 21 strains by next-generation sequencing of genomes, followed by searches of assembled reads against the Comprehensive Antibiotic Resistance Database. Strains resistant to erythromycin, penicillins and tetracyclines were isolated from 83.3, 33.3 and 16.6 %, respectively, of infants aged 2 to 8 months with no prior antibiotic treatment. These percentages were100.0, 66.6 and 50.0 %, by 13 to 16 months of age. ARG or polymorphisms associated with antibiotic resistance were the most prevalent and involved genes for macrolide efflux (mel, mefA/E and macB), ribosomal protection [erm(B), tet(M) and tet(O)] and β-lactamase-like proteins. Phylogenetically related strains showing multidrug-resistant phenotypes harboured multidrug efflux ARG. Polymorphic genes associated with antibiotic resistance to drugs affecting DNA replication, folate synthesis, RNA/protein synthesis and regulators of antibiotic stress responses were detected. These data imply that Streptococcus salivarius strains established during maturation of the oral microbiota harbour a diverse array of functional ARG, even in the absence of antibiotic selective pressures, highlighting a potential role for this species in shaping antibiotic susceptibility profiles of oropharyngeal communities.", }