@article{mbs:/content/journal/jmm/10.1099/jmm.0.000362, author = "Peixoto, Renata Stavracakis and Hacker, Elena and Antunes, Camila Azevedo and Weerasekera, Dulanthi and Dias, AA and Martins, Carlos Alberto and Hirata Jr, Raphael and Santos, Kátia Regina Netto dos and Burkovski, Andreas and Mattos-Guaraldi, Ana Luíza", title = "Pathogenic properties of a Corynebacterium diphtheriae strain isolated from a case of osteomyelitis", journal= "Journal of Medical Microbiology", year = "2016", volume = "65", number = "11", pages = "1311-1321", doi = "https://doi.org/10.1099/jmm.0.000362", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000362", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", keywords = "Corynebacterium diphtheriae", keywords = "osteomyelitis", keywords = "Arthritis", keywords = "diphtheria", keywords = "Invasive infections.", abstract = " Corynebacterium diphtheriae is typically recognized as a colonizer of the upper respiratory tract (respiratory diphtheria) and the skin (cutaneous diphtheria). However, different strains of Corynebacterium diphtheriae can also cause invasive infections. In this study, the characterization of a non-toxigenic Corynebacterium diphtheriae strain (designated BR-INCA5015) isolated from osteomyelitis in the frontal bone of a patient with adenoid cystic carcinoma was performed. Pathogenic properties of the strain BR-INCA5015 were tested in a Caenorhabditis elegans survival assay showing strong colonization and killing by this strain. Survival rates of 3.8±2.7 %, 33.6±7.3 % and 0 % were observed for strains ATCC 27010T, ATCC 27012 and BR-INCA5015, respectively, at day 7. BR-INCA5015 was able to colonize epithelial cells, showing elevated capacity to adhere to and survive within HeLa cells compared to other Corynebacterium diphtheriae isolates. Intracellular survival in macrophages (THP-1 and RAW 264.7) was significantly higher compared to control strains ATCC 27010T (non-toxigenic) and ATCC 27012 (toxigenic). Furthermore, the ability of BR-INCA5015 to induce osteomyelitis was confirmed by in vivo assay using Swiss Webster mice.", }