1887

Abstract

Genetic mechanisms of methicillin resistance are still relevant in staphylococci. The aims of this study are to assess the possible exchanges of staphylococcal cassette chromosome (SCC) among isolates of methicillin-resistant staphylococci (MRS) and to check for known or new mutations in A DNA. A total of 35 MRS non-repetitive isolates were recovered, including 20 , 7 , 4 , 2 and 1 isolate each of and Only 16 of the 35 strains were assigned to known SCC types: 7 SCC VII, 6 SCC IV and 3 SCC III, with possible horizontal transfer of the SCC VII from methicillin-resistant to methicillin-susceptible A gene sequencing in ten selected isolates allowed description of nine punctual mutations, seven of which were reported for the first time. The most frequent mutation was G246E, identified in isolates of methicillin-resistant , , and These results emphasized the high degree of genetic diversity of SCC element in MRS and describe new missense mutations in A, which might be important in understanding the evolution of methicillin and new β-lactam resistance.

Keyword(s): CNS , G246E mutation , mecA , MRS , MRSA and SCCmec
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2016-11-16
2020-09-30
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