1887

Abstract

arouses an increasing public health concern, as this non-tuberculous mycobacterium (NTM) has recently been associated with life-threatening cardiac infections. and are genetically very close but recently appeared to present different epidemiological and clinical significance. Therefore, it has become important for laboratories to use adequate techniques allowing precise species identification. To date, most commercially available laboratory assays cannot distinguish them and erroneously identify as . We performed a re-analysis of the 149 strains received by the Belgian National Reference Laboratory using 16S rRNA gene sequencing, representing 25 % of all NTM collected in 2015. We found that represents the majority (=94, 63 %) of the previous . This study reports the large presence of / among Belgian patients infected by an NTM and the predominance of the species among this group. This study also stresses the public health importance of and demonstrates the inability of commonly used laboratory techniques to correctly diagnose these infections.

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2016-11-16
2020-04-03
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References

  1. Achermann Y., Rössle M., Hoffmann M., Deggim V., Kuster S., Zimmermann D. R., Bloemberg G., Hombach M., Hasse B.. 2013; Prosthetic valve endocarditis and bloodstream infection due to Mycobacterium chimaera. J Clin Microbiol51:1769–1773 [CrossRef][PubMed]
    [Google Scholar]
  2. Alhanna J., Purucker M., Steppert C., Grigull-Daborn A., Schiffel G., Gruber H., Borgmann S.. 2012; Mycobacterium chimaera causes tuberculosis-like infection in a male patient with anorexia nervosa. Int J Eat Disord45:450–452 [CrossRef][PubMed]
    [Google Scholar]
  3. Andre E., Belkhir L., Goeminne L., Colmant A., Sottiaux J.-Y., Yildiz H., Ruelle J., Delmée M., Vandercam B., Defour J.-P.. 2015; Immune reconstitution inflammatory syndrome due to Mycobacterium genavense in an HIV-infected patient: impact of rapid species identification by rpoB sequencing on clinical management. JMM Case Rep2:e005007 [CrossRef]
    [Google Scholar]
  4. Bills N. D., Hinrichs S. H., Aden T. A., Wickert R. S., Iwen P. C.. 2009; Molecular identification of Mycobacterium chimaera as a cause of infection in a patient with chronic obstructive pulmonary disease. Diagn Microbiol Infect Dis63:292–295 [CrossRef][PubMed]
    [Google Scholar]
  5. Cassidy P. M., Hedberg K., Saulson A., McNelly E., Winthrop K. L.. 2009; Nontuberculous mycobacterial disease prevalence and risk factors: a changing epidemiology. Clin Infect Dis49:e124e129 [CrossRef][PubMed]
    [Google Scholar]
  6. Cayrou C., Turenne C., Behr M. A., Drancourt M.. 2010; Genotyping of Mycobacterium avium complex organisms using multi spacer sequence typing. Microbiology156:687–694 [CrossRef][PubMed]
    [Google Scholar]
  7. Cohen-Bacrie S., David M., Stremler N., Dubus J.-C., Rolain J.-M., Drancourt M.. 2011; Mycobacterium chimaera pulmonary infection complicating cystic fibrosis: a case report. J Med Case Rep5:473 [CrossRef][PubMed]
    [Google Scholar]
  8. De Zwaan R., Van Ingen J., Van Soolingen D.. 2014; Utility of rpoB gene sequencing for identification of nontuberculous mycobacteria in the Netherlands. J Clin Microbiol52:2544–2551 [CrossRef][PubMed]
    [Google Scholar]
  9. European Centre for Disease Prevention and Control 2015; Invasive cardiovascular infection by Mycobacterium chimaera. Solna, Sweden: ECDC. http://ecdc.europa.eu/en/publications/publications/mycobacterium-chimaera-infection-associated-with-heater-cooler-units-rapid-risk-assessment-30-april-2015.pdf
  10. Falkinham J. O.. 1996; Epidemiology of infection by nontuberculous mycobacteria. Clin Microbiol Rev9:177–215[PubMed]
    [Google Scholar]
  11. Griffith D. E., Aksamit T., Brown-Elliott B. A., Catanzaro A., Daley C., Gordin F., Holland S. M., Horsburgh R., Huitt G. et al. 2007; An official ATS/IDSA statement: diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases. Am J Respir Crit Care Med175:367–416 [CrossRef][PubMed]
    [Google Scholar]
  12. Haller S., Höller C., Jacobshagen A., Hamouda O., Abu Sin M., Monnet D. L., Plachouras D., Eckmanns T.. 2016; Contamination during production of heater-cooler units by Mycobacterium chimaera potential cause for invasive cardiovascular infections: results of an outbreak investigation in Germany, April 2015 to February 2016. Euro Surveill21:e30215 [CrossRef]
    [Google Scholar]
  13. Hoefsloot W., van Ingen J., Andrejak C., Angeby K., Bauriaud R., Bemer P., Beylis N., Boeree M. J., Cacho J. et al. 2013; The geographic diversity of nontuberculous mycobacteria isolated from pulmonary samples: an NTM-NET collaborative study. Eur Respir J42:1604–1613 [CrossRef][PubMed]
    [Google Scholar]
  14. Kirschner P., Springer B., Vogel U., Meier A., Wrede A., Kiekenbeck M., Bange F. C., Böttger E. C.. 1993; Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory. J Clin Microbiol31:2882–2889[PubMed]
    [Google Scholar]
  15. Kohler P., Kuster S. P., Bloemberg G., Schulthess B., Frank M., Tanner F. C., Rössle M., Böni C., Falk V. et al. 2015; Healthcare-associated prosthetic heart valve, aortic vascular graft, and disseminated Mycobacterium chimaera infections subsequent to open heart surgery. Eur Heart J36:2745–2753 [CrossRef][PubMed]
    [Google Scholar]
  16. Public Health England 2015; Mycobacterial infections associated withheater cooler units used in cardiothoracic surgery. London: Public Health England. https://www.gov.uk/government/collections/mycobacterial-infections-associated-with-heater-cooler-units
  17. Prevots D. R., Marras T. K.. 2015; Epidemiology of human pulmonary infection with nontuberculous mycobacteria: a review. Clin Chest Med36:13–34 [CrossRef][PubMed]
    [Google Scholar]
  18. Ramis I. B., Cnockaert M., Von Groll A., Mathys V., Simon A., Tortoli E., Palomino J. C., Almeida da Silva P. E., Vandamme P. et al. 2015; Evaluation of the Speed-Oligo Mycobacteria assay for the identification of nontuberculous mycobacteria. J Med Microbiol64:283–287 [CrossRef][PubMed]
    [Google Scholar]
  19. Sax H., Bloemberg G., Hasse B., Sommerstein R., Kohler P., Achermann Y., Rössle M., Falk V., Kuster S. P. et al. 2015; Prolonged outbreak of Mycobacterium chimaera infection after open-chest heart surgery. Clin Infect Dis61:67–75 [CrossRef][PubMed]
    [Google Scholar]
  20. Schweickert B., Goldenberg O., Richter E., Göbel U. B., Petrich A., Buchholz P., Moter A.. 2008; Occurrence and clinical relevance of Mycobacterium chimaera sp. nov., Germany. Emerg Infect Dis14:1443–1446 [CrossRef][PubMed]
    [Google Scholar]
  21. Sommerstein R., Rüegg C., Kohler P., Bloemberg G., Kuster S. P., Sax H.. 2016; Transmission of Mycobacterium chimaera from heater–cooler units during cardiac surgery despite an ultraclean air ventilation system. Emerg Infect Dis22:1008–1013 [CrossRef][PubMed]
    [Google Scholar]
  22. Tortoli E., Rindi L., Garcia M. J., Chiaradonna P., Dei R., Garzelli C., Kroppenstedt R. M., Lari N., Mattei R. et al. 2004; Proposal to elevate the genetic variant MAC-A, included in the Mycobacterium avium complex, to species rank as Mycobacterium chimaera sp. nov. Int J Syst Evol Microbiol54:1277–1285 [CrossRef][PubMed]
    [Google Scholar]
  23. Van Ingen J., Hoefsloot W., Buijtels P. C., Tortoli E., Supply P., Dekhuijzen P. N., Boeree M. J., van Soolingen D.. 2012; Characterization of a novel variant of Mycobacterium chimaera. J Med Microbiol61:1234–1239 [CrossRef][PubMed]
    [Google Scholar]
  24. Winthrop K. L., McNelley E., Kendall B., Marshall-Olson A., Morris C., Cassidy M., Saulson A., Hedberg K.. 2010; Pulmonary nontuberculous mycobacterial disease prevalence and clinical features: an emerging public health disease. Am J Respir Crit Care Med182:977–982 [CrossRef][PubMed]
    [Google Scholar]
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