This study aimed to determine the occurrence of Mannheimiahaemolytica, Pasteurella multocida and Mycoplasma spp., in relation to clinical signs of respiratory disease. Tracheobronchial lavage samples were collected from 96 (healthy and unhealthy) cattle in the State of São Paulo, Brazil. Mycoplasma spp. (12.5 %) and Pasteurellamultocida (15.50 %) were the most prevalent species. Bacillus sp., Staphylococcus sp., Escherichia coli, Klebsiella oxytoca, Pseudomonas aeruginosa and Klebsiella pneumoniae were also isolated. Mollicutes (70.83 %), Mycoplasmabovis (2.94 %) and Mycoplasma dispar (38.23 %) were identified using conventional PCR. Submassive sound on acoustic percussion of the thorax was associated with the absence of Mollicutes (P=0.025). Whistling (P=0.076) and coarse crackle (P=0.046) were associated with the absence of Mycoplasma dispar. Clear sound on acoustic percussion of the thorax was associated with the absence of Mycoplasmabovis (P=0.007). Coughing was associated with the presence of Pasteurellamultocida [P=0.035; confidence interval (CI), 1.12–26.89], but its absence was associated with mucopurulent (P=0.0215; CI, 1.55–34.5) and mucoid nasal discharge (P=0.068; CI, 19–28.5), submassive sound (P=0.031; CI, 1.23–75.5), fine crackle (P=0.058; CI, 1.23–20.1) and coarse crackle (P=0.046; CI, 2.38–70.8). The high prevalence of Pasteurella multocida and Mycoplasma spp. in unhealthy calves increases the importance of these micro-organisms in the pathogenesis of respiratory diseases. This study increases the information about the role of Mycoplasma dispar in respiratory diseases. Differences in some species in relation to clinical signs can be applied as a presumptive diagnosis.
AebiM.,
van den BorneB. H.,
RaemyA.,
SteinerA.,
PiloP.,
BodmerM.2015; Mycoplasmabovis infections in Swiss dairy cattle: a clinical investigation. Acta Vet Scand 57:1–11 [View Article][PubMed]
AkanM.,
BabacanO.,
TorunE.,
MustakH. K.,
OncelT.2014; Diagnosis of Mycoplasmabovis infection in cattle by ELISA and PCR. KAFKAS Univ Vet Fak Derg 20:249–252
AlemayehuG.,
LetaS.,
HailuB.2015; Sero-prevalence of contagious bovine pleuropneumonia (CBPP) in bulls originated from Borena pastoral area of Southern Ethiopia. Trop Anim Health Prod 47:983–987 [View Article][PubMed]
AlhajiN. B.,
BabalobiO. O.2015; Sero-positivity and associated risk factors for contagious bovine pleuropneumonia under two cattle production systems in North Central Nigeria. Trop Anim Health Prod 48:311–320 [View Article][PubMed]
AngenO.,
ThomsenJ.,
LarsenL. E.,
LarsenJ.,
KokotovicB.,
HeegaardP. M. H.,
EnemarkJ. M. D.2009; Respiratory disease in calves: microbiological investigations on trans-tracheally aspirated bronchoalveolar fluid and acute phase protein response. Vet Microbio 137:165–171[CrossRef]
BarrosM. S. R. M.,
CastroR. S.,
TabosaJ. H. C.,
BritoM. F.,
AmaralB.1994; Colheita do fluído brônquico-alveolar de bezerros através da traqueocêntese transcutânea. Arq Bras Med Vet Zootec 46:41–49
BenesiF. J.,
BertagnonH. G.,
WachholzL.,
LealM. L. R.,
FernandesW. R.,
BenitesN. R.,
MelvilleP. A.2013; Microbiota bacteriana e citologia da região traqueobrônquica de bezerros no período neonatal. Pesqui Vet Bras 33:700–704 [View Article]
Castillo-AlcalaF.,
BatemanK. G.,
CaiH. Y.,
SchottC. R.,
ParkerL.,
ClarkM. E.,
McRaildP.,
McDowallR. M.,
FosterR. A. et al.2012; Prevalence and genotype of Mycoplasma bovis in beef cattle after arrival at a feedlot. Am J Vet Res 73:1932–1943 [View Article][PubMed]
Chávez GonzálezY. R.,
Ros BascuñanaC.,
BölskeG.,
MattssonJ. G.,
Fernández MolinaC.,
JohanssonK. E.1995; In vitro amplification of the 16S rRNA genes from Mycoplasmabovis and Mycoplasmaagalactiae by PCR. Vet Microbiol 47:183–190 [View Article][PubMed]
de JongA.,
ThomasV.,
SimjeeS.,
MoyaertH.,
El GarchF.,
MaherK.,
MorrisseyI.,
ButtyP.,
KleinU. et al.2014; Antimicrobial susceptibility monitoring of respiratory tract pathogens isolated from diseased cattle and pigs across Europe: the vetpath study. Vet Microbiol 172:202–215 [View Article][PubMed]
DedieuL.,
MadyV.,
LefevreP. C.1994; Development of a selective polymerase chain reaction assay for the detection of Mycoplasmamycoides subsp. mycoides S.C. (contagious bovine pleuropneumonia agent). Vet Microbiol 42:327–339 [View Article][PubMed]
FrancozD.,
BuczinskiS.,
BélangerA. M.,
FortéG.,
LabrecqueO.,
TremblayD.,
WellemansV.,
DubucJ.2015; Respiratory pathogens in Québec dairy calves and their relationship with clinical status, lung consolidation, and average daily gain. J Vet Intern Med 29:381–387 [View Article][PubMed]
GonçalvesR. C.1987Estudo Da Flora Traqueobrônquica Em Bezerros Clinicamente Sadios E Portadores De Pneumonia, Na Região De Botucatu, SP Faculdade de Medicina Veterinária e Zootecnia: Universidade Estadual Paulista, Brazil (in Portuguese);
GriffinD.2010; Bovine pasteurellosis and other bacterial infections of the respiratory tract. Vet Clin North Am Food Anim Pract 26:57–71 [View Article][PubMed]
GriffinD.,
ChengappaM. M.,
KuszakJ.,
McVeyD. S.2010; Bacterial pathogens of the bovine respiratory disease complex. Vet Clin North Am Food Anim Pract 26:381–394 [View Article][PubMed]
HolmanD. B.,
McAllisterT. A.,
ToppE.,
WrightA. D.,
AlexanderT. W.2015; The nasopharyngeal microbiota of feedlot cattle that develop bovine respiratory disease. Vet Microbiol 180:90–95 [View Article][PubMed]
HorwoodP. F.,
SchibrowskiM. I.,
FowlerE. V.,
GibsonJ. S.,
BarnesT. S.,
MahonyT. J.2014; Is Mycoplasmabovis a missing component of the bovine respiratory disease complex in Australia?. Aust Vet J 92:185–191 [View Article][PubMed]
MarquesL. M.,
BuzinhaniM.,
YamagutiM.,
OliveiraR. C.,
FerreiraJ. B.,
MettifogoE.,
TimenetskyJ.2007a; Use of a polymerase chain reaction for detection of Mycoplasmadispar in the nasal mucus of calves. J Vet Diagn Invest 19:103–106 [View Article]
MarquesL. M.,
BuzinhaniM.,
OliveiraR. C.,
YamagutiM.,
FerreiraJ. B.,
NetoR. L.,
TimenetskyJ.,
MettifogoJ. T.2007b; Prevalence of mycoplasmas in the respiratory tracts of calves in Brazil. Vet Rec 161:699–700 [View Article]
MilesD. G.2009; Overview of the North American beef cattle industry and the incidence of bovine respiratory disease (BRD). Anim Health Res Rev 10:101–103 [View Article][PubMed]
MooreS. J.,
O'DeaM. A.,
PerkinsN.,
O'HaraA. J.2015; Estimation of nasal shedding and seroprevalence of organisms known to be associated with bovine respiratory disease in Australian live export cattle. J Vet Diagn Invest 27:6–17 [View Article][PubMed]
RadostitsO. M.,
GayC. C.,
BloodD. C.,
HinchcliffK. W.2007Veterinary Medice: A Textbook of the Diseases of Cattle, Horses, Sheep, Pigs and Goats, 10th edn. Philadelphia: Elsevier;
ŠiugždaitėJ.,
GabinaitienėA.,
ŠiugždaR.2015; Mycoplasmadispar prevalence in the upper respiratory tract of cattle and the antimicrobial susceptibility of the isolates. Chem Technol 65:doi: 10.5755/j01.ct.65.1.8716[CrossRef]
SoehnlenM. K.,
AydinA.,
MurthyK. S.,
LengerichE. J.,
HattelA. L.,
HouserB. A.,
FentonG. D.,
LysczekH. R.,
BurnsC. M. et al.2012; Epidemiology of Mycoplasmabovis in Pennsylvania veal calves. J Dairy Sci 95:247–254 [View Article][PubMed]
SrivastavaN. C.,
ThiaucourtF.,
SinghV. P.,
SunderJ.,
SinghV. P.2000; Isolation of Mycoplasmamycoides small colony type from contagious caprine pleuropneumonia in India. Vet Rec 147:520–521 [View Article][PubMed]
TullyJ. G.1995; Culture medium formulation for primary isolation and maintenance of mollicutes. In Molecular and Diagnostic Procedures in Mycoplasmology pp 33–40 . Edited by
TullyJ. G.,
RazinS.
San Diego: Academic Press Inc; [CrossRef]
USDA2013; Feedlot 2001. Part IV: Health and Health Management on U.S. Feedlots with a capacity of 1000 or more head. In APHIS Fort Collins: National Animal Health Monitoring System;
van KuppeveldF. J.,
van der LogtJ. T.,
AnguloA. F.,
van ZoestM. J.,
QuintW. G.,
NiestersH. G.,
GalamaJ. M.,
MelchersW. J.1992; Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification. Appl Environ Microbiol 58:2606–2615[PubMed]
VianaL.,
GonçalvesR. C.,
Oliveira FilhoJ. P.,
PaesA. C.,
AmorimR. M.2007; Ocorrência de Mannheimiahaemolytica e de Pasteurella multocida em ovinos sadios e com enfermidade respiratória. Arquivo Brasileiro De Medicina Veterinária E Zootecnia 59:1579–1582 [View Article]