1887

Abstract

This study aimed to examine occurrence and antimicrobial resistance characteristics of from pigs, pork and humans in Thailand and Laos provinces. The samples were collected from pigs, carcasses and workers in slaughterhouses, retail pork and butchers in fresh markets and patients in hospitals in Thailand (=729) and Laos (=458). A total of 295 of 729 samples (34.6 %) collected in Thailand and 253 of 458 (47.4 %) samples collected in Laos were positive for . A total of 548 isolates from Thailand (=295) and Laos (=253) were further analysed. Serovar Typhimurium was the most common serotype in Thai (34 %) and Laos (20.6 %) samples. Approximately 2.4 % of Thai isolates produced extended-spectrum β-lactamase (ESBL). All the ESBL producers possessed , some of which were horizontally transferred. Class 1 integrons were common in Thai (31.9 %) and Laos (39.1 %) isolates, but none were associated with SGI1. The resistance cassette was the most common, while the least common was (=1). The gene cassette in five isolates and were located on conjugative plasmid. Three pork isolates were fluoroquinolone resistant and carried an amino acid substitute, Ser-83-Tyr, in GyrA. The gene was found in 7.1 and 5.5 % of the Thai and Laos isolates, respectively, while was carried in another Laos isolate (1.9 %). All ESBL producers carried In conclusion, multidrug-resistant was common in pigs, pork and human samples in this region. The bacteria carried mobile genetic elements and resistance genes on conjugative plasmids that could be readily transferred to other bacterial species.

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2016-10-18
2021-10-20
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