1887

Abstract

The emergence of extensively drug-resistant (XDR) has become a major challenge worldwide. In this study, we characterized the phenotypes and genetic features of nine XDR isolates from an integrated hospital in Zhejiang province, China, from September to October 2014. These XDR possessed at least five resistance determinants which contribute to highly resistant to β-lactam, β-lactam/inhibitor combinations, aminoglycosides, quinolones, carbapenems, chloroamphenicol and fosfomycin. All isolates carried , , and , and several isolates also harboured and . Southern blot experiments confirmed that , and were located on the same ~54.2 kb plasmid. Conjugative plasmids were obtained from all isolates, further proving the transferable characteristic of the resistance determinants. The OmpK36 sequences showed various deletions and insertions that indicated additional amino acid residues and a deleted phenotype of OmpK36. PFGE demonstrated that all the isolates belonged to the same genotype. Multilocus sequence typing was concordant with PFGE results and revealed that ST11 was the most predominant clone. Our study revealed a high incidence and endemic spread of XDR in the hospital. Thus, effective infection control measures should be adopted to monitor and control the spread of multidrug-resistant isolates.

Keyword(s): Klebsiella pneumoniae , Outbreak , ST11 and XDR
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2016-10-18
2021-10-20
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