1887

Abstract

The spread of carbapenem-resistant spp. has become a global problem. In this study, 18 carbapenem-resistant (ACB) complexes, identified using a conventional biochemical method at our hospital during 2004–2013, were studied for species identification and epidemiological analyses. Species identification was performed using matrix-assisted laser desorption ionization–time-of-flight MS, a partial sequence analysis of and a PCR-based ORF typing (POT) method. The POT method can not only identify the species of ACB complexes but also simultaneously determine the international epidemic clones and the genetic identities of in several hours. Carbapenem resistance gene detection by PCR, molecular epidemiological analysis by PFGE and Pasteur Institute multilocus sequence typing (MLST) analysis were performed. All three methods identified 18 isolates as (=10), (=4) and (=4). A metallo-β-lactamase gene in all strains of and and an IS gene in the upstream of the gene in eight strains of were detected, respectively, as carbapenemase-related genes. Results from PFGE demonstrated that nine strains of were closely related genetically. Results of MLST analysis showed that are classifiable to sequence type 2. These results were consistent with those obtained using the POT method. This POT method can easily and rapidly identify the international epidemic clones and the identities of . It can be a useful tool for infection control.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000314
2016-09-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/65/9/923.html?itemId=/content/journal/jmm/10.1099/jmm.0.000314&mimeType=html&fmt=ahah

References

  1. CLSI 2012 Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Second Informational Supplement M100-S22 Wayne, PA: Clinical and Laboratory Standards Institute;
    [Google Scholar]
  2. Diancourt L., Passet V., Nemec A., Dijkshoorn L., Brisse S. 2010; The population structure of Acinetobacter baumannii: expanding multiresistant clones from an ancestral susceptible genetic pool. PLoS One 5:e10034 [View Article][PubMed]
    [Google Scholar]
  3. Dijkshoorn L., Nemec A., Seifert H. 2007; An increasing threat in hospitals: multidrug-resistant Acinetobacter baumannii. Nat Rev Microbiol 5:939–951 [View Article][PubMed]
    [Google Scholar]
  4. Hsueh P. R., Kuo L. C., Chang T. C., Lee T. F., Teng S. H., Chuang Y. C., Teng L. J., Sheng W. H. 2014; Evaluation of the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry system for identification of blood isolates of Acinetobacter species. J Clin Microbiol 52:3095–3100 [View Article][PubMed]
    [Google Scholar]
  5. Kishii K., Kikuchi K., Matsuda N., Yoshida A., Okuzumi K., Uetera Y., Yasuhara H., Moriya K. 2014; Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for species identification of Acinetobacter strains isolated from blood cultures. Clin Microbiol Infect 20:424–430 [View Article][PubMed]
    [Google Scholar]
  6. Kouyama Y., Harada S., Ishii Y., Saga T., Yoshizumi A., Tateda K., Yamaguchi K. 2012; Molecular characterization of carbapenem-non-susceptible Acinetobacter spp. in Japan: predominance of multidrug-resistant Acinetobacter baumannii clonal complex 92 and IMP-type metallo-β-lactamase-producing non-baumannii Acinetobacter species. J Infect Chemother 18:522–528 [View Article][PubMed]
    [Google Scholar]
  7. Kuo S. C., Chang S. C., Wang H. Y., Lai J. F., Chen P. C., Shiau Y. R., Huang I. W., Lauderdale T. L.TSAR Hospitals 2012; Emergence of extensively drug-resistant Acinetobacter baumannii complex over 10 years: nationwide data from the Taiwan Surveillance of Antimicrobial Resistance (TSAR) program. BMC Infect Dis 12:200 [View Article][PubMed]
    [Google Scholar]
  8. La Scola B., Gundi V. A. K. B., Khamis A., Raoult D. 2006; Sequencing of the rpoB gene and flanking spacers for molecular identification of Acinetobacter species. J Clin Microbiol 44:827–832 [View Article][PubMed]
    [Google Scholar]
  9. Lee J. H., Choi C. H., Kang H. Y., Lee J. Y., Kim J., Lee Y. C., Seol S. Y., Cho D. T., Kim K. W. et al. 2007; Differences in phenotypic and genotypic traits against antimicrobial agents between Acinetobacter baumannii and Acinetobacter genomic species 13TU. J Antimicrob Chemother 59:633–639 [View Article][PubMed]
    [Google Scholar]
  10. Lee Y., Kim C. K., Lee H., Jeong S. H., Yong D., Lee K. 2011; A novel insertion sequence, ISAba10, inserted into ISAba1 adjacent to the bla(OXA-23) gene and disrupting the outer membrane protein gene carO in Acinetobacter baumannii. Antimicrob Agents Chemother 55:361–363 [View Article][PubMed]
    [Google Scholar]
  11. Lu P. L., Doumith M., Livermore D. M., Chen T. P., Woodford N. 2009; Diversity of carbapenem resistance mechanisms in Acinetobacter baumannii from a Taiwan hospital: spread of plasmid-borne OXA-72 carbapenemase. J Antimicrob Chemother 63:641–647 [View Article][PubMed]
    [Google Scholar]
  12. Master R. N., Deane J., Opiela C., Sahm D. F. 2013; Recent trends in resistance to cell envelope-active antibacterial agents among key bacterial pathogens. Ann N Y Acad Sci 1277:1–7 [View Article][PubMed]
    [Google Scholar]
  13. Matsui M., Suzuki S., Yamane K., Suzuki M., Konda T., Arakawa Y., Shibayama K. 2014; Distribution of carbapenem resistance determinants among epidemic and non-epidemic types of Acinetobacter species in Japan. J Med Microbiol 63:870–877 [View Article][PubMed]
    [Google Scholar]
  14. Park Y. K., Jung S. I., Park K. H., Kim D. H., Choi J. Y., Kim S. H., Ko K. S. 2012; Changes in antimicrobial susceptibility and major clones of Acinetobacter calcoaceticus-baumannii complex isolates from a single hospital in Korea over 7 years. J Med Microbiol 61:71–79 [View Article][PubMed]
    [Google Scholar]
  15. Peleg A. Y., Seifert H., Paterson D. L. 2008; Acinetobacter baumannii: emergence of a successful pathogen. Clin Microbiol Rev 21:538–582 [View Article][PubMed]
    [Google Scholar]
  16. Perez F., Hujer A. M., Hujer K. M., Decker B. K., Rather P. N., Bonomo R. A. 2007; Global challenge of multidrug-resistant Acinetobacter baumannii. Antimicrob Agents Chemother 51:3471–3484 [View Article][PubMed]
    [Google Scholar]
  17. Poirel L., Nordmann P. 2006; Genetic structures at the origin of acquisition and expression of the carbapenem-hydrolyzing oxacillinase gene blaOXA-58 in Acinetobacter baumannii. Antimicrob Agents Chemother 50:1442–1448 [View Article][PubMed]
    [Google Scholar]
  18. Shibata N., Doi Y., Yamane K., Yagi T., Kurokawa H., Shibayama K., Kato H., Kai K., Arakawa Y. 2003; PCR typing of genetic determinants for metallo-β-lactamases and integrases carried by gram-negative bacteria isolated in Japan, with focus on the class 3 integron. J Clin Microbiol 41:5407–5413 [View Article][PubMed]
    [Google Scholar]
  19. Suzuki M., Hosoba E., Matsui M., Arakawa Y. 2014; New PCR-based open reading frame typing method for easy, rapid, and reliable identification of Acinetobacter baumannii international epidemic clones without performing multilocus sequence typing. J Clin Microbiol 52:2925–2932 [View Article][PubMed]
    [Google Scholar]
  20. Tenover F. C., Arbeit R. D., Goering R. V., Mickelsen P. A., Murray B. E., Persing D. H., Swaminathan B. 1995; Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 33:2233–2239[PubMed]
    [Google Scholar]
  21. Turton J. F., Ward M. E., Woodford N., Kaufmann M. E., Pike R., Livermore D. M., Pitt T. L. 2006; The role of ISAba1 in expression of OXA carbapenemase genes in Acinetobacter baumannii. FEMS Microbiol Lett 258:72–77 [View Article][PubMed]
    [Google Scholar]
  22. Vila J., Martí S., Sánchez-Céspedes J. 2007; Porins, efflux pumps and multidrug resistance in Acinetobacter baumannii. J Antimicrob Chemother 59:1210–1215 [View Article][PubMed]
    [Google Scholar]
  23. Xu T., Xia W., Rong G., Pan S., Huang P., Gu B. 2013; A 4-year surveillance of antimicrobial resistance patterns of Acinetobacter baumanni in a university-affiliated hospital in China. J Thorac Dis 5:506–512 [View Article][PubMed]
    [Google Scholar]
  24. Yamada Y., Suwabe A. 2013; Diverse carbapenem-resistance mechanisms in 16S rRNA methylase-producing Acinetobacter baumannii. J Med Microbiol 62:618–622 [View Article][PubMed]
    [Google Scholar]
  25. Zarrilli R., Pournaras S., Giannouli M., Tsakris A. 2013; Global evolution of multidrug-resistant Acinetobacter baumannii clonal lineages. Int J Antimicrob Agents 41:11–19 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.000314
Loading
/content/journal/jmm/10.1099/jmm.0.000314
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error