1887

Abstract

Rapid and accurate detection of carbapenemase-producing is pivotal for adequate antibiotic therapy and infection control. The Cepheid Xpert Carba-R assay detects and identifies the most prevalent carbapenemases (KPC, VIM, IMP, NDM and OXA-48), using automated real-time PCR. The test performance of the Xpert Carba-R was evaluated with 129 well-characterized non-repeat isolates, suspected for carbapenemase production, i.e. with meropenem MICs >0.25 mg l. The isolate collection contained 100 carbapenemase-producing isolates (36 KPC-2 or KPC-3, 20 VIM-1, 4 KPC-2 plus VIM-1, 5 NDM-1, 2 IMP-1, 1 IMP-28, 1 IMP-1 plus VIM-1 and 31 OXA-48 like) and 29 negative control isolates producing extended-spectrum β-lactamase and/or AmpC β-lactamases. PCR and sequencing of β-lactamase genes were used as reference tests. The sensitivity of the Xpert Carba-R was 100 % (100/100), with a 100 % (29/29) specificity. The time to result was approximately 55 min with a hands-on time of only 1 min per isolate. In conclusion, the Carba-R assay is a rapid and accurate instrument for the confirmation and identification of the , , , and genes.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000310
2016-09-01
2024-11-12
Loading full text...

Full text loading...

/deliver/fulltext/jmm/65/9/951.html?itemId=/content/journal/jmm/10.1099/jmm.0.000310&mimeType=html&fmt=ahah

References

  1. Decousser J. W., Poirel L., Desroches M., Jayol A., Denamur E., Nordmann P. 2015; Failure to detect carbapenem-resistant Escherichia coli producing OXA-48-like using the Xpert Carba-R assay®. Clin Microbiol Infect 21:e910 [View Article][PubMed]
    [Google Scholar]
  2. Doi Y., Paterson D. L. 2015; Carbapenemase-producing Enterobacteriaceae. Semin Respir Crit Care Med 36:74–84 [View Article][PubMed]
    [Google Scholar]
  3. Falagas M. E., Tansarli G. S., Karageorgopoulos D. E., Vardakas K. Z. 2014; Deaths attributable to carbapenem-resistant Enterobacteriaceae infections. Emerg Infect Dis 20:1170–1175 [View Article][PubMed]
    [Google Scholar]
  4. Findlay J., Hopkins K. L., Meunier D., Woodford N. 2015; Evaluation of three commercial assays for rapid detection of genes encoding clinically relevant carbapenemases in cultured bacteria. J Antimicrob Chemother 70:1338–1342 [View Article][PubMed]
    [Google Scholar]
  5. Nordmann P., Poirel L., Dortet L. 2012; Rapid detection of carbapenemase-producing Enterobacteriaceae. Emerg Infect Dis 18:1503–1507 [View Article][PubMed]
    [Google Scholar]
  6. Package insert Cepheid Gene Xpert Carba-R assay 2014 Xpert Carba-R. Ref. GXCARBAR-CE-10 2–28
  7. Pournaras S., Köck R., Mossialos D., Mellmann A., Sakellaris V., Stathopoulos C., Friedrich A. W., Tsakris A. 2013; Detection of a phylogenetically distinct IMP-type metallo-β-lactamase, IMP-35, in a CC235 Pseudomonas aeruginosa from the Dutch-German border region (Euregio). J Antimicrob Chemother 68:1271–1276 [View Article][PubMed]
    [Google Scholar]
  8. Tängdén T., Giske C. G. 2015; Global dissemination of extensively drug-resistant carbapenemase-producing Enterobacteriaceae: clinical perspectives on detection, treatment and infection control. J Intern Med 277:501–512 [View Article][PubMed]
    [Google Scholar]
  9. van Dijk K., Voets G. M., Scharringa J., Voskuil S., Fluit A. C., Rottier W. C., Leverstein-Van Hall M. A., Cohen Stuart J. W. 2014; A disc diffusion assay for detection of class A, B and OXA-48 carbapenemases in Enterobacteriaceae using phenyl boronic acid, dipicolinic acid and temocillin. Clin Microbiol Infect 20:345–349 [View Article][PubMed]
    [Google Scholar]
  10. Vlek A. L., Frentz D., Haenen A., Bootsma H. J., Notermans D. W., Frakking F. N., de Greeff S. C., Leenstra T.ISIS-AR study group 2016; Detection and epidemiology of carbapenemase producing Enterobacteriaceae in the Netherlands in 2013-2014. Eur J Clin Microbiol Infect Dis 35:1089–1096 [View Article][PubMed]
    [Google Scholar]
  11. Voets G. M., Fluit A. C., Scharringa J., Cohen Stuart J., Leverstein-van Hall M. A, Cohen S. J. 2011; A set of multiplex PCRs for genotypic detection of extended-spectrum β-lactamases, carbapenemases, plasmid-mediated AmpC β-lactamases and OXA β-lactamases. Int J Antimicrob Agents 37:356–359 [View Article][PubMed]
    [Google Scholar]
/content/journal/jmm/10.1099/jmm.0.000310
Loading
/content/journal/jmm/10.1099/jmm.0.000310
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error