RT Journal Article SR Electronic(1) A1 Lara-Oya, Ana A1 Rodríguez-Granger, Javier Moisés A1 Liébana-Martos, María del Carmen A1 Mendoza-López, Pablo A1 Sampedro-Martínez, Antonio A1 Cobo, Fernando A1 Gutierrez-Fernández, José A1 Martínez-Lirola, Miguel José A1 Navarro-Marí, José MaríaYR 2016 T1 Preliminary evaluation of a new kit for differentiation of Mycobacterium tuberculosis complex species using Speed-Oligo MTBC JF Journal of Medical Microbiology, VO 65 IS 9 SP 910 OP 914 DO https://doi.org/10.1099/jmm.0.000308 PB Microbiology Society, SN 1473-5644, AB We present the first evaluation of a novel molecular assay, the Speed-Oligo Mycobacterium tuberculosis complex (SO-MTBC), which is based on PCR combined with a dipstick for the differentiation of M. tuberculosis complex (MTBC) members. The results of this assay were compared with findings obtained using the Genotype MTBC assay. In this study, 189 strains of MTBC isolates from 2011 to 2014 were evaluated to determine the MTBC species. Most (174, 92 %) of the strains were identified as M. tuberculosis sensu stricto, 7 (3.7 %) as Mycobacterium bovis, 5 (2.6 %) as M. bovis bacillus Calmette–Guérin, 2 (1.1 %) as Mycobacterium africanum and 1 (0.5 %) as Mycobacterium caprae; no strains belonged to Mycobacterium microti and Mycobacterium canettii subsp. The concordance κ coefficient obtained was 0.96 with the results of the Genotype MTBC assay. SO-MTBC may represent a fast and easy-to-use alternative for differentiating among MTBC subspecies in laboratories with standard equipment., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000308