1887

Abstract

can be part of the upper respiratory flora of animals, but under conditions of stress or immunocompromisation, the bacteria can cause severe respiratory symptoms. In this study, we compared 10 isolates from Icelandic sheep with respiratory symptoms and 19 isolates from apparently healthy abattoir sheep. We examined capsule type, genetic variability and the presence of the gene in the two groups. Surprisingly, we found that all ovine isolates examined in this study carried the gene, which markedly differs from what has been published from other studies. Interestingly, all isolates from abattoir animals were capsule type D, whilst bacteria isolated from animals with clinical respiratory symptoms had capsule type A, D or F. Examination of seven housekeeping genes indicated that the clinical respiratory isolates were significantly more heterogeneous than the abattoir isolates (<0.05, two-tailed Mann–Whitney test). The results suggest that there may be at least two groups of in sheep – a genetically homogeneous group that resides in the respiratory tract and a genetically heterogeneous group that is the predominant cause of disease.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000306
2016-09-01
2019-12-11
Loading full text...

Full text loading...

/deliver/fulltext/jmm/65/9/897.html?itemId=/content/journal/jmm/10.1099/jmm.0.000306&mimeType=html&fmt=ahah

References

  1. Beneduzi A., Costa P. B., Parma M., Melo I. S., Bodanese-Zanettini M. H., Passaglia L. M.. 2010; Paenibacillus riograndensis sp. nov., a nitrogen-fixing species isolated from the rhizosphere of Triticum aestivum . Int J Syst Evol Microbiol60:128–133 [CrossRef][PubMed]
    [Google Scholar]
  2. Bethe A., Wieler L. H., Selbitz H.-J., Ewers C.. 2009; Genetic diversity of porcine Pasteurella multocida strains from the respiratory tract of healthy and diseased swine. Vet Microbiol139:97–105 [CrossRef][PubMed]
    [Google Scholar]
  3. Boyce J. D., Chung J. Y., Adler B.. 2000; Pasteurella multocida capsule: composition, function and genetics. J Biotechnol83:153–160 [CrossRef][PubMed]
    [Google Scholar]
  4. Boyce J. D, Seemann T., Adler B., Harper M.. 2012; Pathogenomics of Pasteurella multocida . In Pasteurella Multocida (Curr Top Microbiol Immunol)vol. 361 , pp.23–38 Edited by Aktories K., Orth J. H. C., Adler B.. Berlin: : Springer;[CrossRef]
    [Google Scholar]
  5. Brogden K. A., Rose D., Cutlip R. C., Lehmkuhl H. D., Tully J. G.. 1988; Isolation and identification of mycoplasmas from the nasal cavity of sheep. Am J Vet Res49:1669–1672[PubMed]
    [Google Scholar]
  6. Cheville N. F., Rimler R. B.. 1989; A protein toxin from Pasteurella multocida type D causes acute and chronic hepatic toxicity in rats. Vet Pathol26:148–157 [CrossRef][PubMed]
    [Google Scholar]
  7. Choi C., Chae C.. 2001; Enhanced detection of toxigenic Pasteurella multocida directly from nasal swabs using a nested polymerase chain reaction. Vet J162:255–258 [CrossRef][PubMed]
    [Google Scholar]
  8. Chrisp C., Foged N.. 1991; Induction of pneumonia in rabbits by use of a purified protein toxin from Pasteurella multocida . Am J Vet Res52:56–61[PubMed]
    [Google Scholar]
  9. Dabo S. M., Taylor J. D., Confer A. W.. 2007; Pasteurella multocida and bovine respiratory disease. Anim Health Res Rev8:129–150 [CrossRef][PubMed]
    [Google Scholar]
  10. Davies R. L., MacCorquodale R., Baillie S., Caffrey B.. 2003; Characterization and comparison of Pasteurella multocida strains associated with porcine pneumonia and atrophic rhinitis. J Med Microbiol52:59–67 [CrossRef][PubMed]
    [Google Scholar]
  11. DeAngelis P. L., Gunay N. S., Toida T., Mao W. J., Linhardt R. J.. 2002; Identification of the capsular polysaccharides of type D and F Pasteurella multocida as unmodified heparin and chondroitin, respectively. Carbohydr Res337:1547–1552 [CrossRef][PubMed]
    [Google Scholar]
  12. Deressa A., Asfaw Y., Lubke B., Kyule M. W., Tefera G., Zessin K. H.. 2010; Molecular detection of Pasteurella multocida and Mannheimia haemolytica in sheep respiratory infections in Ethiopia. J Appl Res Vet Med8:101–108
    [Google Scholar]
  13. Donnio P. Y., Avril J. L., Andre P. M., Vaucel J.. 1991; Dermonecrotic toxin production by strains of Pasteurella multocida isolated from man. J Med Microbiol34:333–337 [CrossRef][PubMed]
    [Google Scholar]
  14. Essler M., Hermann K., Amano M., Kaibuchi K., Heesemann J., Weber P. C., Aepfelbacher M.. 1998; Pasteurella multocida toxin increases endothelial permeability via Rho kinase and myosin light chain phosphatase. J Immunol161:5640–5646[PubMed]
    [Google Scholar]
  15. Ewers C., Lübke-Becker A., Bethe A., Kiebling S., Filter M., Wieler L. H.. 2006; Virulence genotype of Pasteurella multocida strains isolated from different hosts with various disease status. Vet Microbiol114:304–317 [CrossRef][PubMed]
    [Google Scholar]
  16. Fridriksdottir V., Gunnarsson E., Sigurdarson S., Gudmundsdottir K. B.. 2003; Paratuberculosis in Iceland – ongoing research. Acta Vet Scand44:273[PubMed]
    [Google Scholar]
  17. Fujiwara-Nagata E., Chantry-Darmon C., Bernardet J.-F., Eguchi M., Duchaud E., Nicolas P.. 2013; Population structure of the fish pathogen Flavobacterium psychrophilum at whole-country and model river levels in Japan. Vet Res44:34 [CrossRef][PubMed]
    [Google Scholar]
  18. Gudnadóttir M., Demosthenous A., Hadjisavvas T.. 2013; Vaccination delays maedi-visna lentivirus infection in a naturally-infected sheep flock. BMC Vet Res9: [CrossRef][PubMed]
    [Google Scholar]
  19. Harper M., Boyce J. D., Adler B.. 2006; Pasteurella multocida pathogenesis: 125 years after Pasteur. FEMS Microbiol Lett265:1–10 [CrossRef][PubMed]
    [Google Scholar]
  20. Hatfaludi T., Al-Hasani K., Boyce J. D., Adler B.. 2010; Outer membrane proteins of Pasteurella multocida . Vet Microbiol144:1–17 [CrossRef][PubMed]
    [Google Scholar]
  21. Hoskins I. C., Lax A. J.. 1996; Constitutive expression of Pasteurella multocida toxin. FEMS Microbiol Lett141:189–193 [CrossRef][PubMed]
    [Google Scholar]
  22. Jaglic Z., Kucerova Z., Nedbalcova K., Pavlik I., Alexa P., Bartos M.. 2005; Characterisation and comparison of Pasteurella multocida isolated from different species in the Czech Republic: capsular PCR typing, ribotyping and dermonecrotoxin production. Vet Med – Czech50:345–354
    [Google Scholar]
  23. Jarvinen L. Z., Hogenesch H., Suckow M. A., Bowersock T. L.. 1998; Induction of protective immunity in rabbits by coadministration of inactivated Pasteurella multocida toxin and potassium thiocyanate extract. Infect Immun66:3788–3795[PubMed]
    [Google Scholar]
  24. Kim T., Son C., Lee J., Kim K.. 2012; Vaccine potential of an attenuated Pasteurella multocida that expresses only the N-terminal truncated fragment of P. multocida toxin in pigs. Can J Vet Res76:69–71[PubMed]
    [Google Scholar]
  25. Kubatzky K. F., Kloos B., Hildebrand D.. 2013; Signaling cascades of Pasteurella multocida toxin in immune evasion. Toxins5:1664–1681 [CrossRef][PubMed]
    [Google Scholar]
  26. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A. et al. 2007; Clustal W and Clustal X version 2.0. Bioinformatics23:2947–2948 [CrossRef][PubMed]
    [Google Scholar]
  27. Lee J., Kang H.-E., Woo H.-J.. 2012; Protective immunity conferred by the C-terminal fragment of recombinant Pasteurella multocida toxin. Clin Vaccine Immunol19:1526–1531 [CrossRef][PubMed]
    [Google Scholar]
  28. Liu W., Yang M., Xu Z., Zheng H., Liang W., Zhou R., Wu B., Chen H.. 2012; Complete genome sequence of Pasteurella multocida HN06, a toxigenic strain of serogroup D. J Bacteriol194:3292–3293 [CrossRef][PubMed]
    [Google Scholar]
  29. Michael G. B., Kadlec K., Sweeney M. T., Brzuszkiewicz E., Liesegang H., Daniel R., Murray R. W., Watts J. L., Schwarz S.. 2012; ICEPmu1, an integrative conjugative element (ICE) of Pasteurella multocida: analysis of the regions that comprise 12 antimicrobial resistance genes. J Antimicrob Chemother67:84–90 [CrossRef][PubMed]
    [Google Scholar]
  30. Orth J. H., Aktories K.. 2010; Pasteurella multocida toxin activates various heterotrimeric G proteins by deamidation. Toxins2:205–214 [CrossRef][PubMed]
    [Google Scholar]
  31. Petersen S. K., Foged N. T., Bording A., Nielsen J. P., Riemann H. K., Frandsen P. L.. 1991; Recombinant derivatives of Pasteurella multocida toxin: candidates for a vaccine against progressive atrophic rhinitis. Infect Immun59:1387–1393[PubMed]
    [Google Scholar]
  32. Prabhakar T. G., Prabhakar P., Venkadabadi. 2010; Molecular characterization of Pasteurella multocida isolated from an incidence of sheep pasteurellosis in Karamadai hill tract of Tamil Nadu. Vet Anim Sci6:81–87
    [Google Scholar]
  33. Pullinger G. D., Bevir T., Lax A. J.. 2004; The Pasteurella multocida toxin is encoded within a lysogenic bacteriophage. Mol Microbiol51:255–269 [CrossRef][PubMed]
    [Google Scholar]
  34. Pálsson P. A.. 1972; Maedi-visna. J Clin Pathol6:115–120[CrossRef]
    [Google Scholar]
  35. Quinn P. J.. 1994; Pasteurella species. In Clinical Veterinary Microbiology , pp.254–259 Edited by Quinn P. J., Carter M. E., Markey B. K., Carter G. R.. UK: Wolfe Publishing;
    [Google Scholar]
  36. Seo J., Pyo H., Lee S., Lee J., Kim T.. 2009; Expression of 4 truncated fragments of Pasteurella multocida toxin and their immunogenicity. Can J Vet Res73:184–189[PubMed]
    [Google Scholar]
  37. Shayegh J., Atashpaz S., Hejazi M. S.. 2008; Virulence genes profile and typing of ovine Pasteurella multocida . Asian J Anim Vet Adv3:206–213 [CrossRef]
    [Google Scholar]
  38. Shayegh J., Parvizi M., Hejazi M. S.. 2010; Diversity of caprine and ovine Pasteurella multocida isolates based on 16S rRNA gene sequencing. Iran J Vet Res11:373–378
    [Google Scholar]
  39. Siegert P., Schmidt G., Papatheodorou P., Wieland T., Aktories K., Orth J. H.. 2013; Pasteurella multocida toxin prevents osteoblast differentiation by transactivation of the MAP-kinase cascade via the Gα(q/11) - p63RhoGEF - RhoA axis. PLoS Pathog9:e1003385 [CrossRef][PubMed]
    [Google Scholar]
  40. Sterner-Kock A., Lanske B., Uberschär S., Atkinson M. J.. 1995; Effects of the Pasteurella multocida toxin on osteoblastic cells in vitro . Vet Pathol32:274–279 [CrossRef][PubMed]
    [Google Scholar]
  41. Storz J., Lin X., Purdy C. W., Chouljenko V. N., Kousoulas K. G., Enright F. M., Gilmore W. C., Briggs R. E., Loan R. W.. 2000; Coronavirus and Pasteurella infections in bovine shipping fever pneumonia and Evans' criteria for causation. J Clin Microbiol38:3291–3298[PubMed]
    [Google Scholar]
  42. Subaaharan S., Blackall L. L., Blackall P. J.. 2010; Development of a multi-locus sequence typing scheme for avian isolates of Pasteurella multocida . Vet Microbiol141:354–361 [CrossRef][PubMed]
    [Google Scholar]
  43. Surguy S. M., Duricki D. A., Reilly J. M., Lax A. J., Robbins J.. 2014; The actions of Pasteurella multocida toxin on neuronal cells. Neuropharmacology77:9–18 [CrossRef][PubMed]
    [Google Scholar]
  44. Tahamtan Y., Hayati M., Namavari M. M.. 2014; Isolation and identification of Pasteurella multocida by PCR from sheep and goats in Fars province, Iran. Archives of Razi Institute69:89–93
    [Google Scholar]
  45. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  46. To H., Someno S., Nagai S.. 2005; Development of a genetically modified nontoxigenic Pasteurella multocida toxin as a candidate for use in vaccines against progressive atrophic rhinitis in pigs. Am J Vet Res66:113–118 [CrossRef][PubMed]
    [Google Scholar]
  47. Townsend K. M., Boyce J. D., Chung J. Y., Frost A. J., Adler B.. 2001; Genetic organization of Pasteurella multocida cap loci and development of a multiplex capsular PCR typing system. J Clin Microbiol39:924–929 [CrossRef][PubMed]
    [Google Scholar]
  48. Verma S., Sharma M., Katoch S., Verma L., Kumar S., Dogra V., Chahota R., Dhar P., Singh G.. 2013; Profiling of virulence associated genes of Pasteurella multocida isolated from cattle. Vet Res Commun37:83–89 [CrossRef][PubMed]
    [Google Scholar]
  49. Weber D. J., Wolfson J. S., Swartz M. N., Hooper D. C.. 1984; Pasteurella multocida infections. Report of 34 cases and review of the literature. Medicine63:133–154[PubMed][CrossRef]
    [Google Scholar]
  50. Weiser G. C., DeLong W. J., Paz J. L., Shafii B., Price W. J., Ward A. C.. 2003; Characterization of Pasteurella multocida associated with pneumonia in bighorn sheep. J Wildl Dis39:536–544 [CrossRef][PubMed]
    [Google Scholar]
  51. Wilson B. A., Ho M.. 2013; Pasteurella multocida: from zoonosis to cellular microbiology. Clin Microbiol Rev26:631–655 [CrossRef][PubMed]
    [Google Scholar]
  52. Zamri-Saad M., Effendy W. M., Maswati M. A., Salim N., Sheikh-Omar A. R.. 1996; The goat as a model for studies of pneumonic pasteurellosis caused by Pasteurella multocida . Br Vet J152:453–458 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.000306
Loading
/content/journal/jmm/10.1099/jmm.0.000306
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error