1887

Abstract

The genus is characterized by hyaline filamentous fungi that cause infections predominantly in immunocompromised patients. The remarkable primary resistance to antifungal agents of this genus requires a search for new therapeutic possibilities. This study assessed the susceptibility of 25 clinical isolates of against antifungal agents (amphotericin B, caspofungin, itraconazole and voriconazole) and antimicrobials (pentamidine, polymyxin B, tigecycline and tobramycin) according to the broth microdilution method (M38-A2). The interactions between antifungal and antimicrobial agents were evaluated by the microdilution checkerboard method. Pentamidine and polymyxin B showed MIC values ≥4 µg ml against spp. The highest rates of synergism were observed when amphotericin B or voriconazole was combined with tobramycin (80 % and 76 %, respectively), polymyxin B (76 % and 64 %) and pentamidine (72 % and 68 %). The most significant combinations deserve evaluations in order to verify their potential in the treatment of fusariosis.

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2016-08-01
2021-10-22
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References

  1. Afeltra J., Dannaoui E., Meis J. F., Rodriguez-Tudela J. L., Verweij P. E., Network E. 2002; In vitro synergistic interaction between amphotericin B and pentamidine against Scedosporium prolificans . Antimicrob Agents Chemother 46:3323–3326 [View Article][PubMed]
    [Google Scholar]
  2. Al-Hatmi A. M., Meis J. F., de Hoog G. S. 2016; Fusarium: molecular diversity and intrinsic drug resistance. PLoS Pathog 12:e1005464 [View Article][PubMed]
    [Google Scholar]
  3. Beringer P. M., Vinks A. A., Jelliffe R. W., Shapiro B. J. 2000; Pharmacokinetics of tobramycin in adults with cystic fibrosis: implications for once-daily administration. Antimicrob Agents Chemother 44:809–813 [View Article][PubMed]
    [Google Scholar]
  4. Bernard E. M., Donnelly H. J., Maher M. P., Armstrong D. 1985; Use of a new bioassay to study pentamidine pharmacokinetics. J Infect Dis 152:750–754 [View Article][PubMed]
    [Google Scholar]
  5. Chodosh J., Miller D., Tu E. Y., Culbertson W. W. 2000; Tobramycin-responsive Fusarium oxysporum keratitis. Can J Ophthalmol 35:29–30 [View Article][PubMed]
    [Google Scholar]
  6. Clancy C. J., Nguyen M. H. 1998; The combination of amphotericin B and azithromycin as a potential new therapeutic approach to fusariosis. J Antimicrob Chemother 41:127–130 [View Article][PubMed]
    [Google Scholar]
  7. CLSI (2008). Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi; Approved Standard, 2nd edn. Wayne, PA: Clinical and Laboratory Standards Institute;
    [Google Scholar]
  8. Dalhoff A. 1987; Pleiotropic actions of aminoglycosides. Antibiot Chemother 39:182–204[PubMed] [CrossRef]
    [Google Scholar]
  9. Day S., Lalitha P., Haug S., Fothergill A. W., Cevallos V., Vijayakumar R., Prajna N. V., Acharya N. R., McLeod S. D., Lietman T. M. 2009; Activity of antibiotics against Fusarium and Aspergillus . Br J Ophthalmol 93:116–119 [View Article][PubMed]
    [Google Scholar]
  10. Espinel-Ingroff A. 2008; Mechanisms of resistance to antifungal agents: yeasts and filamentous fungi. Rev Iberoam Micol 25:101–106[PubMed] [CrossRef]
    [Google Scholar]
  11. Espinel-Ingroff A., Colombo A. L., Cordoba S., Dufresne P. J., Fuller J., Ghannoum M., Gonzalez G. M., Guarro J., Kidd S. E. et al. 2016; International evaluation of MIC distributions and epidemiological cutoff value (ECV) definitions for Fusarium species identified by molecular methods for the CLSI broth microdilution method. Antimicrob Agents Chemother 60:1079–1084 [View Article]
    [Google Scholar]
  12. Jesus F. P., Ferreiro L., Loreto É. S., Pilotto M. B., Ludwig A., Bizzi K., Tondolo J. S., Zanette R. A., Alves S. H., Santurio J. M. 2014; In vitro synergism obtained by azithromycin, clarithromycin, minocycline or tigecycline in association with antifungal agents against Pythium insidiosum . Antimicrob Agents Chemother 58:5621–5625 [View Article][PubMed]
    [Google Scholar]
  13. Johnson M. D., MacDougall C., Ostrosky-Zeichner L., Perfect J. R., Rex J. H. 2004; Combination antifungal therapy. Antimicrob Agents Chemother 48:693–715 [View Article][PubMed]
    [Google Scholar]
  14. Ku T. S., Palanisamy S. K., Lee S. A. 2010; Susceptibility of Candida albicans biofilms to azithromycin, tigecycline and vancomycin and the interaction between tigecycline and antifungals. Int J Antimicrob Agents 36:441–446 [View Article][PubMed]
    [Google Scholar]
  15. Lionakis M. S., Lewis R. E., Samonis G., Kontoyiannis D. P. 2003; Pentamidine is active in vitro against Fusarium species. Antimicrob Agents Chemother 47:3252–3259 [View Article][PubMed]
    [Google Scholar]
  16. Marras T. K., Sanders K., Lipton J. H., Messner H. A., Conly J., Chan C. K. 2002; Aerosolized pentamidine prophylaxis for Pneumocystis carinii pneumonia after allogeneic marrow transplantation. Transpl Infect Dis 4:66–74 [View Article][PubMed]
    [Google Scholar]
  17. Mason A. J., Marquette A., Bechinger B. 2007; Zwitterionic phospholipids and sterols modulate antimicrobial peptide-induced membrane destabilization. Biophys J 93:4289–4299 [View Article][PubMed]
    [Google Scholar]
  18. Moody J. 2007; Synergism testing: Broth microdilution checkerboard and broth macrodilution methods. In Clinical Microbiology Procedures Handbook , pp. 1–23 Edited by Garcia L. S., Isenberg H. D. Washington: ASM Press;
    [Google Scholar]
  19. Muhammed M., Coleman J. J., Carneiro H. A., Mylonakis E. 2011; The challenge of managing fusariosis. Virulence 2:91–96 [View Article][PubMed]
    [Google Scholar]
  20. Nucci M., Anaissie E. 2007; Fusarium infections in immunocompromised patients. Clin Microbiol Rev 20:695–704 [View Article][PubMed]
    [Google Scholar]
  21. O'Donnell K., Sutton D. A., Rinaldi M. G., Sarver B. A., Balajee S. A., Schroers H. J., Summerbell R. C., Robert V. A., Crous P. W. et al. 2010; Internet-accessible DNA sequence database for identifying fusaria from human and animal infections. J Clin Microbiol 48:3708–3718 [View Article][PubMed]
    [Google Scholar]
  22. Pankey G. A. 2005; Tigecycline. J Antimicrob Chemother 56:470–480 [View Article][PubMed]
    [Google Scholar]
  23. Reyes M. P., Zhao J. J., Buensalido J. A. 2014; Current perspectives: therapeutic uses of tobramycin. J Pharmacovigil 2:1–5
    [Google Scholar]
  24. Rodvold K. A., Gotfried M. H., Cwik M., Korth-Bradley J. M., Dukart G., Ellis-Grosse E. J. 2006; Serum, tissue and body fluid concentrations of tigecycline after a single 100 mg dose. J Antimicrob Chemother 58:1221–1229 [View Article][PubMed]
    [Google Scholar]
  25. Sands M., Kron M. A., Brown R. B. 1985; Pentamidine: a review. Rev Infect Dis 7:625–634 [View Article][PubMed]
    [Google Scholar]
  26. Sarria J. C., Angulo-Pernett F., Kimbrough R. C., McVay C. S., Vidal A. M. 2004; Use of intravenous polymyxin B during continuous venovenous hemodialysis. Eur J Clin Microbiol Infect Dis 23:340–341 [View Article][PubMed]
    [Google Scholar]
  27. St-Germain G. 1990; Effects of pentamidine alone and in combination with ketoconazole or itraconazole on the growth of Candida albicans . Antimicrob Agents Chemother 34:2304–2306 [View Article][PubMed]
    [Google Scholar]
  28. Torres H. A., Kontoyiannis D. P. 2003; Hyalohyphomycoses. In Oxford Textbook of Clinical Mycology , pp. 252–270 Edited by Dismukes W. E. , Pappas P. G., Sobel J. D. New York: Oxford University Press;
    [Google Scholar]
  29. Tortorano A. M., Richardson M., Roilides E., van Diepeningen A., Caira M., Munoz P., Johnson E., Meletiadis J., Pana Z. D. et al. 2014; ESCMID and ECMM joint guidelines on diagnosis and management of hyalohyphomycosis: Fusarium spp., Scedosporium spp. and others. Clin Microbiol Infect 20:27–46 [View Article][PubMed]
    [Google Scholar]
  30. White T. J., Burns T., Lee S., Taylor J. W. 1990; Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: A Guide to Methods and Applications , pp. 315–322 Edited by Innis M. A., Gelfand D. H. , Sninsky J. J., White T. J. New York: Academic Press;
    [Google Scholar]
  31. Zavascki A. P., Goldani L. Z., Li J., Nation R. L. 2007; Polymyxin B for the treatment of multidrug-resistant pathogens: a critical review. J Antimicrob Chemother 60:1206–1215 [View Article][PubMed]
    [Google Scholar]
  32. Zhai B., Zhou H., Yang L., Zhang J., Jung K., Giam C. Z., Xiang X., Lin X. 2010; Polymyxin B, in combination with fluconazole, exerts a potent fungicidal effect. J Antimicrob Chemother 65:931–938 [View Article][PubMed]
    [Google Scholar]
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