1887

Abstract

is associated with various human diseases such as periodontal disease and colorectal cancer (CRC); thus, detection might serve as a novel diagnostic tool. Here, we describe the development of a sensitive and rapid molecular method for detecting two genes: the highly conserved and , which encode a critical host colonization factor. Loop-mediated isothermal amplification (LAMP) primer sets for the rapid detection of and were designed and optimized. The primers yielded consistent negative results for 20 non- bacterial strains, confirming the high specificity of the primers. LAMP reaction primer sensitivity was determined, and its detection rate in comparison to conventional PCR was assessed using 57 clinical stool samples. The LAMP detection limit for and was 22.5 and 0.225 pg µl, respectively, indicating that the sensitivity of this method was 10-fold higher than that of conventional PCR. These results suggest that the LAMP technique is able to effectively identify via as well as detect its virulence factor. To the best of our knowledge, this study is the first to report the application of LAMP for the detection of and in . The LAMP method constitutes a sensitive and specific visual assay for the rapid detection of the pathogen .

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2016-08-01
2019-12-15
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