First evidence of polar flagella in isolated from a patient with neonatal sepsis Free

Abstract

The genus belongs to the family Enterobacteriaceae, and is currently considered to be non-motile and non-flagellated. In the present work, 25 strains isolated from nosocomial infections were assessed for motility under different growth conditions. One isolate, KpBUAP021, demonstrated a swim-like motility phenotype. The genotype was confirmed by 16S rRNA and gene sequence analysis. Multilocus sequence typing analysis also revealed that the KpBUAP021 strain places it in the ST345 sequence type, and belongs to the phylogenetic Kpl group. Transmission electron microscopy and the Ryu staining technique revealed that KpBUAP021 expresses polar flagella. Finally, the presence of , and genes in this strain was confirmed. This report presents the first evidence for flagella-mediated motility in a clinical isolate, and represents an important finding related to its evolution and pathogenic potential.

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2016-08-01
2024-03-29
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