1887

Abstract

Meticillin-resistant (MRSA) bloodstream infection is responsible for significant morbidity, with mortality rates as high as 60 % if not treated appropriately. We describe a rapid method to detect MRSA in blood cultures using a combined three-hour short-incubation BRUKER matrix-assisted laser desorption/ionization time-of-flight MS BioTyper protocol and a qualitative immunochromatographic assay, the Alere Culture Colony Test PBP2a detection test. We compared this combined method with a molecular method detecting the and genes currently performed in our laboratory. One hundred and seventeen blood cultures were tested of which 35 were MRSA and 82 were meticillin-sensitive (MSSA). The rapid combined test correctly identified 100 % (82/82) of the MSSA and 85.7 % (30/35) of the MRSA after 3 h. There were five false negative results where the isolates were correctly identified as , but PBP2a was not detected by the Culture Colony Test. The combined method has a sensitivity of 87.5 %, specificity of 100 %, a positive predictive value of 100 % and a negative predictive value of 94.3 % with the prevalence of MRSA in our blood cultures. The combined rapid method offers a significant benefit to early detection of MRSA in positive blood cultures.

Keyword(s): Bacteremia , MALDI-ToF , MRSA , PBP2a and Rapid
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2016-07-01
2020-03-29
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