1887

Abstract

The genus is known to possess the capacity to acquire and disseminate antimicrobial resistant determinants alongside the ability to produce various virulence genes that enables it to establish infections. We assessed the prevalence and antibiogram profiles of spp. in faecal samples of dairy cattle. Faecal swab samples were collected from 400 dairy cattle from two commercial cattle farms in two rural communities in the Eastern Cape, South Africa. Confirmation of enterococci isolates was carried out by PCR targeting of the gene. Species delineation was by species-specific primers targeting the superoxide dismutase ( A) gene in a multiplex PCR assay. Isolates were screened for the presence of the following virulence genes (, E, , A, A and E) and antimicrobial resistance determinants to erythromycin, vancomycin and streptomycin were evaluated molecularly. A total of 340 isolates were confirmed as belonging to the genus . Species distribution among the isolates consisted of (52.94 %) and (23.53 %) in preponderance compared to the three other species, namely (8.8 %), (8.6 %) and (5.9 %). All were resistant to vancomycin, while 99 % showed resistance to aminoglycoside and 94 % to macrolide. Three virulence genes (, E and ) were detected in almost all the confirmed isolates. The resistance determinants B (19.7 %), C1 (25 %), C2/3 (26.3 %) B (40.29 %) and A (50.88 %) were detected among the isolates. A high prevalence of multidrug-resistant enterococci isolates was detected in this study and the genetic repertoire to survive in the presence of antimicrobial agents was present in these organisms.

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2016-07-01
2021-07-28
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