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Volume 65, Issue 7

Curing vector for IncI1 plasmids and its use to provide evidence for a metabolic burden of IncI1 CTX-M-1 plasmid pIFM3791 on Free

Abstract

Using a sequence-based approach we previously identified an IncI1 CTX-M-1 plasmid, pIFM3791, on a single pig farm in the UK that was harboured by , and serotype 4,5,12:i:-. To test the hypothesis that the plasmid had spread rapidly into these differing host bacteria we wished to assess whether the plasmid conferred a fitness advantage. To do this an IncI1 curing vector was constructed and used to displace the IncI1 CTX-M-1 plasmids from strain B3791 and several other unrelated IncI1-harbouring strains indicating the potential wider application of the curing vector. The IncI1 CTX-M-1 plasmid was reintroduced by conjugation into the cured strain and also a naturally IncI1 plasmid free serotype 4,5,12:i:-, S348/11. Original, cured and complemented strains were tested for metabolic competence using Biolog technology and in competitive growth, association to mammalian cells and biofilm formation experiments. The plasmid-cured strain grew more rapidly than either the original plasmid-carrying strain or plasmid-complemented strains in competition experiments. Additionally, the plasmid-cured strain was significantly better at respiring with -sorbose as a carbon source and putrescine, γ-amino-n-butyric acid, -alanine and -proline as nitrogen sources. By contrast, no differences in phenotype were found when comparing plasmid-harbouring and plasmid-free S348/11. In conclusion, the IncI1 curing vector successfully displaced multiple IncI plasmids. The IncI1 CTX-M1 plasmid conferred a growth disadvantage upon , possibly by imposing a metabolic burden, the mechanism of which remains to be determined.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Enterobacteriaceae , ESBL , IncI1 curing vector and Veterinary microbiology
© 2016 (C) Crown Copyright 2016. Published with permission of the Animal and Plant Health Agency.
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2016-07-01
2024-04-23
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Curing vector for IncI1 plasmids and its use to provide evidence for a metabolic burden of IncI1 CTX-M-1 plasmid pIFM3791 on Klebsiella pneumoniae
J. Med. Microbiol. 65, 611 (2016); https://doi.org/10.1099/jmm.0.000271
/content/journal/jmm/10.1099/jmm.0.000271
/content/journal/jmm/10.1099/jmm.0.000271
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