1887

Abstract

Genomic sequencing of several dermatophyte species has revealed that they show small differences in genetic content and genome organization, although each fungus has adapted to specific niches. Thus, it seemed relevant to compare gene expression between species. Here, we examined the transcription modulation of three ATP-binding cassette (ABC) transporter genes (, and ), which code for membrane transporter proteins in four species of ;,, and . These fungal species were challenged with sub-lethal doses of griseofulvin, itraconazole, terbinafine and amphotericin B. A mutant strain of , Δ, was also analysed for the modulation of and genes to evaluate the possible functional interaction among these three genes. Disruption of the gene resulted in the accumulation of high levels of transcripts when challenged with griseofulvin, suggesting that the gene is compensating for the inactivation of by providing resistance to this antifungal. Although the three ABC transporter genes have high homology between the four dermatophytes analysed, it is likely that they have specific functions, suggesting that the action of each drug is dependent on other factors inherent to each species. Our data suggest that these ABC transporter genes act synergistically in dermatophytes, and they may compensate for one another when challenged with antifungal drugs. This may be an important cause of therapeutic failure when treating fungal infections.

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2016-07-01
2019-12-15
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