Comparing apples and oranges: considerations for quantifying candidal biofilms with XTT [2,3-bis(2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide] and the need for standardized testing

References

1. Dhale R. P., Ghorpade M. V., Dharmadhikari C. A. 2014; Comparison of various methods used to detect biofilm production of Candida species. J Clin Diagn Res 8:DC18-c20[PubMed]
   [Google Scholar]
2. Hawser S. 1996a; Adhesion of different Candida spp. to plastic: XTT formazan determinations. J Med Vet Mycol 34:407–410 [View Article][PubMed]
   [Google Scholar]
3. Hawser S. 1996b; Comparisons of the susceptibilities of planktonic and adherent Candida albicans to antifungal agents: a modified XTT tetrazolium assay using synchronised C. albicans cells. J Med Vet Mycol 34:149–152 [View Article][PubMed]
   [Google Scholar]
4. Hawser S. P., Norris H., Jessup C. J., Ghannoum M. A. 1998; Comparison of a 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) colorimetric method with the standardized National Committee for Clinical Laboratory Standards method of testing clinical yeast isolates for susceptibility to antifungal agents. J Clin Microbiol 36:1450–1452[PubMed]
   [Google Scholar]
5. Kuhn D. M., Balkis M., Chandra J., Mukherjee P. K., Ghannoum M. A. 2003; Uses and limitations of the XTT assay in studies of Candida growth and metabolism. J Clin Microbiol 41:506–508 [View Article][PubMed]
   [Google Scholar]
6. Marcos-Zambrano L. J., Escribano P., Bouza E., Guinea J. 2014; Production of biofilm by Candida and non-Candida spp. isolates causing fungemia: comparison of biomass production and metabolic activity and development of cut-off points. Int J Med Microbiol 304:1192–1198 [View Article][PubMed]
   [Google Scholar]
7. Pierce C. G., Uppuluri P., Tristan A. R., Wormley F. L. Jr, Mowat E., Ramage G., Lopez-Ribot J. L. 2008; A simple and reproducible 96-well plate-based method for the formation of fungal biofilms and its application to antifungal susceptibility testing. Nat Protoc 3:1494–1500 [View Article][PubMed]
   [Google Scholar]
8. Pongrácz J., Benedek K., Juhász E., Iván M., Kristóf K. 2015; In vitro biofilm production of Candida bloodstream isolates: any association with clinical characteristics. J Med Microbiol [View Article] (In Press). [PubMed]
   [Google Scholar]
9. Rajendran R., Sherry L., Nile C. J., Sherriff A., Johnson E. M., Hanson M. F., Williams C., Munro C. A., Jones B. J., Ramage G. 2016; Biofilm formation is a risk factor for mortality in patients with Candida albicans bloodstream infection - Scotland, 2012-2013. Clin Microbiol Infect 22:87–93 [View Article][PubMed]
   [Google Scholar]
10. Ramage G., Vande Walle K., Wickes B. L., López-Ribot J. L. 2001; Standardized method for in vitro antifungal susceptibility testing of Candida albicans biofilms. Antimicrob Agents Chemother 45:2475–2479 [View Article][PubMed]
    [Google Scholar]
11. Taff H. T., Nett J. E., Andes D. R. 2012; Comparative analysis of Candida biofilm quantitation assays. Med Mycol 50:214–218 [View Article][PubMed]
    [Google Scholar]
12. Tellier R., Krajden M., Grigoriew G. A., Campbell I. 1992; Innovative endpoint determination system for antifungal susceptibility testing of yeasts. Antimicrob Agents Chemother 36:1619–1625 [View Article][PubMed]
    [Google Scholar]
13. Thein Z. M., Samaranayake Y. H., Samaranayake L. P. 2007; In vitro biofilm formation of Candida albicans and non-albicans Candida species under dynamic and anaerobic conditions. Arch Oral Biol 52:761–767 [View Article][PubMed]
    [Google Scholar]
14. Tumbarello M., Posteraro B., Trecarichi E. M., Fiori B., Rossi M., Porta R., de Gaetano Donati K., La Sorda M., Spanu T., other authors. 2007; Biofilm production by Candida species and inadequate antifungal therapy as predictors of mortality for patients with candidemia. J Clin Microbiol 45:1843–1850 [View Article][PubMed]
    [Google Scholar]
15. Tumbarello M., Fiori B., Trecarichi E. M., Posteraro P., Losito A. R., De Luca A., Sanguinetti M., Fadda G., Cauda R., Posteraro B. 2012; Risk factors and outcomes of candidemia caused by biofilm-forming isolates in a tertiary care hospital. PLoS One 7:e33705 [View Article][PubMed]
    [Google Scholar]