1887

Abstract

Bacterial infections have high incidence among the female population at reproductive age and are widely known to cause infertility due to inflammation. The purpose of the present study was to investigate the effect of the inflammatory agent LPS on fertility outcome and to evaluate the ability of in ameliorating the LPS-mediated inflammation-induced infertility. Female BALB/c mice infused intravaginally with a single dose of 20 μl sterile normal saline containing 5, 10 or 20 μg LPS were divided into two groups for evaluation of tissue histology and pregnancy outcome. In the first group, aimed at observing changes in tissue histology, inflammation was observed in vaginal sections of mice instilled with a single dose of 20 μg LPS, which were sacrificed on days 2, 5 and 8. In the second group, aimed at evaluating pregnancy outcome, female mice were administered 20 μg LPS, which rendered them infertile upon mating on days 2, 5 and 8. In another experiment, normal histology of vaginal sections was observed in mice administered a single dose of 20 μg LPS, followed by 10 c.f.u. in 20 μl at 24 h intervals, until the mice were sacrificed on days 2, 5 and 8. Following similar treatment, female mice, when mated with proven male breeder mice on days 2, 5 and 8, retained their fertility and delivered pups. These results were further confirmed by the downregulation of pro-inflammatory cytokines and an increase in anti-inflammatory cytokines on treatment with , revealing the role of probiotics in ameliorating inflammation-induced infertility.

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2016-05-01
2021-07-27
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References

  1. Antonio M. A. D., Hawes S. E., Hillier S. L. 1999; The identification of vaginal Lactobacillus species and the demographic and microbiologic characteristics of women colonized by these species. J Infect Dis 180:1950–1956 [View Article][PubMed]
    [Google Scholar]
  2. Baecher-Lind L. E., Miller W. C., Wilcox A. J. 2010; Infectious disease and reproductive health: a review. Obstet Gynecol Surv 65:53–65 [View Article][PubMed]
    [Google Scholar]
  3. Bhandari P., Prabha V. 2015; Evaluation of profertility effect of probiotic Lactobacillus plantarum 2621 in a murine model. Indian J Med Res 142:79–84 [View Article][PubMed]
    [Google Scholar]
  4. Bibiloni R., Fedorak R. N., Tannock G. W., Madsen K. L., Gionchetti P., Campieri M., De Simone C., Sartor R. B. 2005; VSL#3 probiotic-mixture induces remission in patients with active ulcerative colitis. Am J Gastroenterol 100:1539–1546 [View Article][PubMed]
    [Google Scholar]
  5. Boris S., Suárez J. E., Vázquez F., Barbés C. 1998; Adherence of human vaginal lactobacilli to vaginal epithelial cells and interaction with uropathogens. Infect Immun 66:1985–1989[PubMed]
    [Google Scholar]
  6. Cannon J. P., Lee T. A., Bolanos J. T., Danziger L. H. 2005; Pathogenic relevance of Lactobacillus: a retrospective review of over 200 cases. Eur J Clin Microbiol Infect Dis 24:31–40 [View Article][PubMed]
    [Google Scholar]
  7. Coskun E., Ozkan S., Vural B. 2005; Impact of genital infections on fertility. J Turk Germ Gynecol Assoc 6:197–203
    [Google Scholar]
  8. Cram L. F., Zapata M. I., Toy E. C., Baker B. 3rd 2002; Genitourinary infections and their association with preterm labor. Am Fam Physician 65:241–248[PubMed]
    [Google Scholar]
  9. Dal Bosco A., Brecchia A., Cardinali R., Castellini C., Boiti C. 2005; Effet d'une infection intra-utérine avec des lipopolysaccharides bactériens (LPS) sur certains aspects de la fonction reproductive de la lapine. In Proceedings of 11èmes Journées de la Recherche Cunicole 29–30 November 2005, Paris pp 103–106
    [Google Scholar]
  10. Deb K., Chaturvedi M. M., Jaiswal Y. K. 2004b; Comprehending the role of LPS in Gram-negative bacterial vaginosis: ogling into the causes of unfulfilled child-wish. Arch Gynecol Obstet 270:133–146 [View Article][PubMed]
    [Google Scholar]
  11. Di Giacinto C., Marinaro M., Sanchez M., Strober W., Boirivant M. 2005; Probiotics ameliorate recurrent Th1-mediated murine colitis by inducing IL-10 and IL-10-dependent TGF-β-bearing regulatory cells. J Immunol 174:3237–3246 [View Article][PubMed]
    [Google Scholar]
  12. Fedorak R. N., Madsen K. L. 2004; Probiotics and the management of inflammatory bowel disease. Inflamm Bowel Dis 10:286–299 [View Article][PubMed]
    [Google Scholar]
  13. Furrie E., Macfarlane S., Kennedy A., Cummings J. H., Walsh S. V., O'neil D. A., Macfarlane G. T. 2005; Synbiotic therapy (Bifidobacterium longum/Synergy 1) initiates resolution of inflammation in patients with active ulcerative colitis: a randomised controlled pilot trial. Gut 54:242–249 [View Article][PubMed]
    [Google Scholar]
  14. Galdiero F., Sommese L., Gorga F., Galdiero E., Rizzo A., Ajello M. 1994; Toxic effect on human spermatozoa by Chlamydia trachomatis purified lipopolysaccharide. FEMS Microbiol Lett 115:197–200 [View Article][PubMed]
    [Google Scholar]
  15. Gorga F., Galdiero M., Buommino E., Galdiero E. 2001; Porins and lipopolysaccharide induce apoptosis in human spermatozoa. Clin Diagn Lab Immunol 8:206–208[PubMed]
    [Google Scholar]
  16. Gupta K., Stapleton A. E., Hooton T. M., Roberts P. L., Fennell C. L., Stamm W. E. 1998; Inverse association of H2O2-producing lactobacilli and vaginal Escherichia coli colonization in women with recurrent urinary tract infections. J Infect Dis 178:446–450 [View Article][PubMed]
    [Google Scholar]
  17. Haggerty C. L., Gottlieb S. L., Taylor B. D., Low N., Xu F., Ness R. B. 2010; Risk of sequelae after Chlamydia trachomatis genital infection in women. J Infect Dis 201:(Suppl. 2)S134–S155 [View Article][PubMed]
    [Google Scholar]
  18. Isolauri E., Salminen S., Nutrition, Allergy, Mucosal Immunology, and Intestinal Microbiota (NAMI) Research Group Report. 2008; Probiotics: use in allergic disorders: a Nutrition, Allergy, Mucosal Immunology, and Intestinal Microbiota (NAMI) Research Group Report. J Clin Gastroenterol 42:(Suppl. 2)S91–S96 [View Article][PubMed]
    [Google Scholar]
  19. Kaur K., Prabha V. 2013; Impairment by sperm agglutinating factor isolated from Escherichia coli: receptor specific interactions. BioMed Res Int 2013:548497 [View Article][PubMed]
    [Google Scholar]
  20. Kokkayil P., Rawre J., Malhotra N., Dhawan B. 2013; Co-infection of Mycoplasma genitalium and Chlamydia trachomatis in an infertile female patient with genital tuberculosis. Indian J Pathol Microbiol 56:457–459 [View Article][PubMed]
    [Google Scholar]
  21. Lepargneur J. P., Rousseau V. 2002; Rôle portecteur de la flore de Doderlein. J Gynecol Obstet Biol Reprod (Paris) 31:458–494
    [Google Scholar]
  22. Pascual L. M., Daniele M. B., Ruiz F., Giordano W., Pájaro C., Barberis L. 2008; Lactobacillus rhamnosus L60, a potential probiotic isolated from the human vagina. J Gen Appl Microbiol 54:141–148 [View Article][PubMed]
    [Google Scholar]
  23. Redondo-Lopez V., Cook R. L., Sobel J. D. 1990; Emerging role of lactobacilli in the control and maintenance of the vaginal bacterial microflora. Rev Infect Dis 12:856–872 [View Article][PubMed]
    [Google Scholar]
  24. Rietschel E. T., Kirikae T., Feist W., Loppnow H., Zabel P., Brade L., Ulmer A. J., Brade H., Seydel U. & other authors 1991; Molecular aspects of the chemistry and biology of endotoxin. In Molecular Aspects of Inflammation Edited by Sies H., Flohe L., Zimmer G. Berlin: Springer Verlag;207–231 [CrossRef]
    [Google Scholar]
  25. van Oostrum N., De Sutter P., Meys J., Verstraelen H. 2013; Risks associated with bacterial vaginosis in infertility patients: a systematic review and meta-analysis. Hum Reprod 28:1809–1815 [View Article][PubMed]
    [Google Scholar]
  26. Velraeds M. M. C., van de Belt-Gritter B., van der Mei H. C., Reid G., Busscher H. J. 1998; Interference in initial adhesion of uropathogenic bacteria and yeasts to silicone rubber by a Lactobacillus acidophilus biosurfactant. J Med Microbiol 47:1081–1085 [View Article][PubMed]
    [Google Scholar]
  27. Wiesenfeld H. C., Hillier S. L., Meyn L. A., Amortegui A. J., Sweet R. L. 2012; Subclinical pelvic inflammatory disease and infertility. Obstet Gynecol 120:37–43 [View Article][PubMed]
    [Google Scholar]
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