1887

Abstract

Many clinically relevant biofilms are polymicrobial. Examining the effect of antimicrobials in a multispecies biofilm consortium is of great clinical importance. The goal of this study was to investigate the effect of different honey types against bacterial wound pathogens grown in multispecies biofilm and to test the antibiofilm activity of honey defensin-1 (Def-1) in its recombinant form. A modified Lubbock chronic wound biofilm formed by four bacterial species (, , and ) was used for evaluation of honey and recombinant bee-derived Def-1 antibiofilm efficacy. Recombinant Def-1 was prepared by heterologous expression in We showed that different types of honey (manuka and honeydew) were able to significantly reduce the cell viability of wound pathogens (, and ) in mature polymicrobial biofilm. None of the tested honeys showed the ability to eradicate in biofilm. In addition, recombinant Def-1 successfully reduced the viability of and cells within established polymicrobial biofilm after 24 and 48 h of treatment. Interestingly, recombinant Def-1 did not affect the viability of cells within the biofilm, whereas both natural honeys significantly reduced the viable bacteria. Although was highly resistant to Def-1, Def-1 significantly affected the biofilm formation of and after 24 h of treatment, most likely by inhibiting its extracellular polymeric substances production. In conclusion, our study revealed that honey and Def-1 are effective against established multispecies biofilm; however, grown in multispecies biofilm was resistant to both antimicrobials.

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2016-04-01
2020-04-04
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