1887

Abstract

Oral candidiasis is a disease caused by opportunistic species of that normally reside on human mucosal surfaces. The transition of from budding yeast to filamentous hyphae allows for covalent attachment to oral epithelial cells, followed by biofilm formation, invasion and tissue damage. In this study, combinations of SD5870, CBS N116411 and DSM 14685 were assessed for their ability to inhibit the formation of and disrupt biofilms. Co-incubation with probiotic supernatants under hyphae-inducing conditions reduced biofilm formation by >75 % in all treatment groups. Likewise, combinations of live probiotics reduced biofilm formation of by >67 %. When live probiotics or their supernatants were overlaid on preformed biofilms, biofilm size was reduced by >63 and >65 % respectively. Quantitative real-time PCR results indicated that the combined supernatants of SD5870 and CBS N116411 significantly reduced the expression of several genes involved in the yeast–hyphae transition: (adhesin/invasin) by 70 % ( < 0.0001), (hyphae-specific gene activator) by 47 % ( = 0.0061), (secreted protease) by 49 % ( < 0.0001) and (hyphal wall protein critical to biofilm formation) by >99 % ( < 0.0001). These findings suggest the combination of SD5870, CBS N116411 and DSM 14685 is effective at both preventing the formation of and removing preformed biofilms. Our novel results point to the downregulation of several genes critical to the yeast–hyphae transition, biofilm formation, tissue invasion and cellular damage.

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2016-04-01
2020-01-19
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