1887

Abstract

is an important global health problem worldwide. There is still scarce information on the population structure of strains in Asia, where the majority of the world population lives. This study characterized the diversity of strains in northern Vietnam through multilocus sequence typing (MLST). Eighty-five carriage isolates from the community and 77 invasive isolates from the clinical setting were selected and tested for meticillin resistance and the presence of Panton–Valentine leukocidin (PVL). MLST was performed on these isolates, of which CC59 (25.4 %), CC188 (17.3 %) and CC45 (16.7 %) were the predominant clonal complexes (CCs). CC59 carriage isolates had significantly lower rates of meticillin-resistant (MRSA) than their corresponding clinical group isolates (32 vs 83 %). There were no significant differences in rates of MRSA between carriage isolates and clinical isolates of CC45 and CC188. CC59 carriage isolates were significantly lower in rates of PVL than CC59 clinical isolates (32 vs 83 %), but the converse was shown in CC45 isolates (14 vs 0 %, respectively). This study revealed vast differences in the molecular epidemiology and population structure of in community and clinical settings in Vietnam. Nevertheless, the data underline the spread of virulent and/or resistant strains (MRSA and/or PVL) in the community, suggesting the necessity for further surveillance to determine the mechanism of transmission of these strains (i.e. MRSA/PVL) outside clinical settings.

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2016-04-01
2020-01-17
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References

  1. Brunel A. S., Bañuls A. L., Marchandin H., Bouzinbi N., Morquin D., Jumas-Bilak E., Corne P.. 2014; Methicillin-sensitive Staphylococcus aureus CC398 in intensive care unit, France. Emerg Infect Dis20:1511–1515 [CrossRef][PubMed]
    [Google Scholar]
  2. Centers for Disease Control and Prevention 1999; Four pediatric deaths from community-acquired methicillin-resistant Staphylococcus aureus — Minnesota and North Dakota, 1997–1999. MMWR Morb Mortal Wkly Rep48:707–710[PubMed]
    [Google Scholar]
  3. Chen X., Wang W. K., Han L. Z., Liu Y., Zhang H., Tang J., Liu Q. Z., Huangfu Y. C., Ni Y. X.. 2013; Epidemiological and genetic diversity of Staphylococcus aureus causing bloodstream infection in Shanghai, 2009–2011. PLoS One8:e72811 [CrossRef][PubMed]
    [Google Scholar]
  4. CLSI 2012; Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard, 11th edn. M02-A11 Wayne, PA: Clinical and Laboratory Standards Institute;
    [Google Scholar]
  5. Cosgrove S. E., Sakoulas G., Perencevich E. N., Schwaber M. J., Karchmer A. W., Carmeli Y.. 2003; Comparison of mortality associated with methicillin-resistant and methicillin-susceptible Staphylococcus aureus bacteremia: a meta-analysis. Clin Infect Dis36:53–59 [CrossRef][PubMed]
    [Google Scholar]
  6. Cuny C., Nathaus R., Layer F., Strommenger B., Altmann D., Witte W.. 2009; Nasal colonization of humans with methicillin-resistant Staphylococcus aureus (MRSA) CC398 with and without exposure to pigs. PLoS One4:e6800 [CrossRef][PubMed]
    [Google Scholar]
  7. de Neeling A. J., van den Broek M. J., Spalburg E. C., van Santen-Verheuvel M. G., Dam-Deisz W. D., Boshuizen H. C., van de Giessen A. W., van Duijkeren E., Huijsdens X. W.. 2007; High prevalence of methicillin resistant Staphylococcus aureus in pigs. Vet Microbiol122:366–372 [CrossRef][PubMed]
    [Google Scholar]
  8. Du J., Chen C., Ding B., Tu J., Qin Z., Parsons C., Salgado C., Cai Q., Song Y., other authors. 2011; Molecular characterization and antimicrobial susceptibility of nasal Staphylococcus aureus isolates from a Chinese medical college campus. PLoS One6:e27328 [CrossRef][PubMed]
    [Google Scholar]
  9. Enright M. C., Day N. P., Davies C. E., Peacock S. J., Spratt B. G.. 2000; Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J Clin Microbiol38:1008–1015[PubMed]
    [Google Scholar]
  10. Feil E. J., Nickerson E. K., Chantratita N., Wuthiekanun V., Srisomang P., Cousins R., Pan W., Zhang G., Xu B., other authors. 2008; Rapid detection of the pandemic methicillin-resistant Staphylococcus aureus clone ST 239, a dominant strain in Asian hospitals. J Clin Microbiol46:1520–1522 [CrossRef][PubMed]
    [Google Scholar]
  11. Galtier N., Gouy M., Gautier C.. 1996; seaview phylo_win: two graphic tools for sequence alignment and molecular phylogeny. Comput Appl Biosci12:543–548[PubMed]
    [Google Scholar]
  12. Geng W., Yang Y., Wu D., Huang G., Wang C., Deng L., Zheng Y., Fu Z., Li C., other authors. 2010; Molecular characteristics of community-acquired, methicillin-resistant Staphylococcus aureus isolated from Chinese children. FEMS Immunol Med Microbiol58:356–362[PubMed]
    [Google Scholar]
  13. Ghaznavi-Rad E., Nor Shamsudin M., Sekawi Z., Khoon L. Y., Aziz M. N., Hamat R. A., Othman N., Chong P. P., van Belkum A., other authors. 2010; Predominance and emergence of clones of hospital-acquired methicillin-resistant Staphylococcus aureus in Malaysia. J Clin Microbiol48:867–872 [CrossRef][PubMed]
    [Google Scholar]
  14. Gonzalez B. E., Martinez-Aguilar G., Hulten K. G., Hammerman W. A., Coss-Bu J., Avalos-Mishaan A., Mason E. O. Jr, Kaplan S. L.. 2005; Severe staphylococcal sepsis in adolescents in the era of community-acquired methicillin-resistant Staphylococcus aureus. Pediatrics115:642–648 [CrossRef][PubMed]
    [Google Scholar]
  15. Guindon S., Dufayard J. F., Lefort V., Anisimova M., Hordijk W., Gascuel O.. 2010; New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol59:307–321 [CrossRef][PubMed]
    [Google Scholar]
  16. Kondo Y., Ito T., Ma X. X., Watanabe S., Kreiswirth B. N., Etienne J., Hiramatsu K.. 2007; Combination of multiplex PCRs for staphylococcal cassette chromosome mec type assignment: rapid identification system for mec, ccr, and major differences in junkyard regions. Antimicrob Agents Chemother51:264–274 [CrossRef][PubMed]
    [Google Scholar]
  17. Li J, Wang L., Ip M., Sun M., Sun J., Huang G., Wang C., Deng L., Zheng Y., other authors. 2013; Molecular and clinical characteristics of clonal complex 59 methicillin-resistant Staphylococcus aureus infections in mainland China. PLoS One8:e70602 [CrossRef][PubMed]
    [Google Scholar]
  18. McClure J. A., Conly J. M., Lau V., Elsayed S., Louie T., Hutchins W., Zhang K.. 2006; Novel multiplex PCR assay for detection of the staphylococcal virulence marker Panton-Valentine leukocidin genes and simultaneous discrimination of methicillin-susceptible from -resistant staphylococci. J Clin Microbiol44:1141–1144 [CrossRef][PubMed]
    [Google Scholar]
  19. Monecke S., Slickers P., Ellington M. J., Kearns A. M., Ehricht R.. 2007; High diversity of Panton-Valentine leukocidin-positive, methicillin-susceptible isolates of Staphylococcus aureus and implications for the evolution of community-associated methicillin-resistant S. aureus. Clin Microbiol Infect13:1157–1164 [CrossRef][PubMed]
    [Google Scholar]
  20. Monecke S., Coombs G., Shore A. C., Coleman D. C., Akpaka P., Borg M., Chow H., Ip M., Jatzwauk L., other authors. 2011; A field guide to pandemic, epidemic and sporadic clones of methicillin-resistant Staphylococcus aureus. PLoS One6:e17936 [CrossRef][PubMed]
    [Google Scholar]
  21. Moore C. L., Osaki-Kiyan P., Perri M., Donabedian S., Haque N. Z., Chen A., Zervos M. J.. 2010; USA600 (ST45) methicillin-resistant Staphylococcus aureus bloodstream infections in urban Detroit. J Clin Microbiol48:2307–2310 [CrossRef][PubMed]
    [Google Scholar]
  22. Nimmo G. R., Coombs G. W.. 2008; Community-associated methicillin-resistant Staphylococcus aureus (MRSA) in Australia. Int J Antimicrob Agents31:401–410 [CrossRef][PubMed]
    [Google Scholar]
  23. Otter J. A., French G. L.. 2010; Molecular epidemiology of community-associated meticillin-resistant Staphylococcus aureus in Europe. Lancet Infect Dis10:227–239 [CrossRef][PubMed]
    [Google Scholar]
  24. Peck K. R., Baek J. Y., Song J. H., Ko K. S.. 2009; Comparison of genotypes and enterotoxin genes between Staphylococcus aureus isolates from blood and nasal colonizers in a Korean hospital. J Korean Med Sci24:585–591 [CrossRef][PubMed]
    [Google Scholar]
  25. Rolo J., Miragaia M., Turlej-Rogacka A., Empel J., Bouchami O., Faria N. A., Tavares A., Hryniewicz W., Fluit A. C., de Lencastre H., CONCORD Working Group. 2012; High genetic diversity among community-associated Staphylococcus aureus in Europe: results from a multicenter study. PLoS One7:e34768 [CrossRef][PubMed]
    [Google Scholar]
  26. Sabet N. S., Subramaniam G., Navaratnam P., Sekaran S. D.. 2007; Detection of methicillin- and aminoglycoside-resistant genes and simultaneous identification of S. aureus using triplex real-time PCR Taqman assay. J Microbiol Methods68:157–162 [CrossRef][PubMed]
    [Google Scholar]
  27. Sheng W. H., Wang J. T., Lauderdale T. L., Weng C. M., Chen D., Chang S. C.. 2009; Epidemiology and susceptibilities of methicillin-resistant Staphylococcus aureus in Taiwan: emphasis on chlorhexidine susceptibility. Diagn Microbiol Infect Dis63:309–313 [CrossRef][PubMed]
    [Google Scholar]
  28. Shibuya Y., Hara M., Higuchi W., Takano T., Iwao Y., Yamamoto T.. 2008; Emergence of the community-acquired methicillin-resistant Staphylococcus aureus USA300 clone in Japan. J Infect Chemother14:439–441 [CrossRef][PubMed]
    [Google Scholar]
  29. Sievers F., Wilm A., Dineen D., Gibson T. J., Karplus K., Li W., Lopez R., McWilliam H., Remmert M., other authors. 2011; Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega. Mol Syst Biol7:539 [CrossRef][PubMed]
    [Google Scholar]
  30. Simor A. E., Ofner-Agostini M., Bryce E., McGeer A., Paton S., Mulvey M. R., Canadian Hospital Epidemiology Committee and Canadian Nosocomial Infection Surveillance Program, Health Canada. 2002; Laboratory characterization of methicillin-resistant Staphylococcus aureus in Canadian hospitals: results of 5 years of National Surveillance, 1995-1999. J Infect Dis186:652–660 [CrossRef][PubMed]
    [Google Scholar]
  31. Song J. H., Hsueh P. R., Chung D. R., Ko K. S., Kang C. I., Peck K. R., Yeom J. S., Kim S. W., Chang H. H., other authors. 2011; Spread of methicillin-resistant Staphylococcus aureus between the community and the hospitals in Asian countries: an ANSORP study. J Antimicrob Chemother66:1061–1069 [CrossRef][PubMed]
    [Google Scholar]
  32. Tang C. T., Nguyen D. T., Ngo T. H., Nguyen T. M., Le V. T., To S. D., Lindsay J., Nguyen T. D., Bach V. C., other authors. 2007; An outbreak of severe infections with community-acquired MRSA carrying the Panton-Valentine leukocidin following vaccination. PLoS One2:e822 [CrossRef][PubMed]
    [Google Scholar]
  33. Tenover F. C., McDougal L. K., Goering R. V., Killgore G., Projan S. J., Patel J. B., Dunman P. M.. 2006; Characterization of a strain of community-associated methicillin-resistant Staphylococcus aureus widely disseminated in the United States. J Clin Microbiol44:108–118 [CrossRef][PubMed]
    [Google Scholar]
  34. Thwaites G. E., United Kingdom Clinical Infection Research Group (UKCIRG). 2010; The management of Staphylococcus aureus bacteremia in the United Kingdom and Vietnam: a multi-centre evaluation. PLoS One5:e14170 [CrossRef][PubMed]
    [Google Scholar]
  35. Udo E. E., Pearman J. W., Grubb W. B.. 1993; Genetic analysis of community isolates of methicillin-resistant Staphylococcus aureus in Western Australia. J Hosp Infect25:97–108 [CrossRef][PubMed]
    [Google Scholar]
  36. van Belkum A., Melles D. C., Nouwen J., van Leeuwen W. B., van Wamel W., Vos M. C., Wertheim H. F., Verbrugh H. A.. 2009; Co-evolutionary aspects of human colonisation and infection by Staphylococcus aureus. Infect Genet Evol9:32–47 [CrossRef][PubMed]
    [Google Scholar]
  37. van Loo I., Huijsdens X., Tiemersma E., de Neeling A., van de Sande-Bruinsma N., Beaujean D., Voss A., Kluytmans J.. 2007; Emergence of methicillin-resistant Staphylococcus aureus of animal origin in humans. Emerg Infect Dis13:1834–1839 [CrossRef][PubMed]
    [Google Scholar]
  38. Van Nguyen K., Zhang T., Thi Vu B. N., Dao T. T., Tran T. K., Thi Nguyen D. N., Thi Tran H. K., Thi Nguyen C. K., Fox A., other authors. 2014; Staphylococcus aureus nasopharyngeal carriage in rural and urban northern Vietnam. Trans R Soc Trop Med Hyg108:783–790 [CrossRef][PubMed]
    [Google Scholar]
  39. Wang J. L., Wang J. T., Sheng W. H., Chen Y. C., Chang S. C.. 2010a; Nosocomial methicillin-resistant Staphylococcus aureus (MRSA) bacteremia in Taiwan: mortality analyses and the impact of vancomycin, MIC = 2 mg/L, by the broth microdilution method. BMC Infect Dis10:159 [CrossRef][PubMed]
    [Google Scholar]
  40. Wang J. T., Wang J. L., Fang C. T., Chie W. C., Lai M. S., Lauderdale T. L., Weng C. M., Chang S. C.. 2010b; Risk factors for mortality of nosocomial methicillin-resistant Staphylococcus aureus (MRSA) bloodstream infection: with investigation of the potential role of community-associated MRSA strains. J Infect61:449–457 [CrossRef][PubMed]
    [Google Scholar]
  41. Wang W. Y., Chiueh T. S., Sun J. R., Tsao S. M., Lu J. J.. 2012; Molecular typing and phenotype characterization of methicillin-resistant Staphylococcus aureus isolates from blood in Taiwan. PLoS One7:e30394 [CrossRef][PubMed]
    [Google Scholar]
  42. Wannet W. J., Spalburg E., Heck M. E., Pluister G. N., Willems R. J., De Neeling A. J.. 2004; Widespread dissemination in The Netherlands of the epidemic berlin methicillin-resistant Staphylococcus aureus clone with low-level resistance to oxacillin. J Clin Microbiol42:3077–3082 [CrossRef][PubMed]
    [Google Scholar]
  43. Wassenberg M. W., Bootsma M. C., Troelstra A., Kluytmans J. A., Bonten M. J.. 2011; Transmissibility of livestock-associated methicillin-resistant Staphylococcus aureus (ST398) in Dutch hospitals. Clin Microbiol Infect17:316–319 [CrossRef][PubMed]
    [Google Scholar]
  44. Wertheim H. F., Melles D. C., Vos M. C., van Leeuwen W., van Belkum A., Verbrugh H. A., Nouwen J. L.. 2005a; The role of nasal carriage in Staphylococcus aureus infections. Lancet Infect Dis5:751–762 [CrossRef][PubMed]
    [Google Scholar]
  45. Wertheim H. F., van Leeuwen W. B., Snijders S., Vos M. C., Voss A., Vandenbroucke-Grauls C. M., Kluytmans J. A., Verbrugh H. A., van Belkum A.. 2005b; Associations between Staphylococcus aureus genotype, infection, and in-hospital mortality: a nested case-control study. J Infect Dis192:1196–1200 [CrossRef][PubMed]
    [Google Scholar]
  46. Witte W., Kresken M., Braulke C., Cuny C.. 1997; Increasing incidence and widespread dissemination of methicillin-resistant Staphylococcus aureus (MRSA) in hospitals in central Europe, with special reference to German hospitals. Clin Microbiol Infect3:414–422 [CrossRef][PubMed]
    [Google Scholar]
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