Over a period of 3 years' study (2012–2014), a total of 518 faecal samples were collected and cultured to isolate Escherichia coli. Of these, 338 (65.3 %) E. coli isolates were recovered from infants, and 142/338 (42 %) were multidrug-resistant (MDR) to ≥ 3 drug classes using the antimicrobial susceptibility disc diffusion method. A total of 125/142 (88 %) of E. coli isolates were extended-spectrum β-lactamase (ESBL) producers. blaCTX-M-15 types were observed in 80/125 (64 %) of the isolates, and 60/80 (75 %) were positive for blaCTX-M-15. Out of 338 E. coli isolates, 9 (2.6 %) were positive for ST131/O25b clone and each isolate was associated with several plasmids of different sizes (1–21.2 kb). The identities of these nine isolates were confirmed by sequencing for presence of pabB (347 bp) and trpA (427 bp) genes. This study demonstrates low prevalence rate of the highly virulent E. coli ST131 clone producing blaCTX-M-15 in the intestines of Jordanian infants.
Abu SalahM.,
BadranE.,
ShehabiA.2013; High incidence of multidrug resistant Escherichia coli producing CTX-M-type ESBLs colonizing the intestine of Jordanian infants. Int Arab J Antimicrob Agents 3:4, 3
BurgessM. J.,
JohnsonJ. R.,
PorterS. B.,
JohnstonB.,
ClabotsC.,
LahrB. D.,
UhlJ. R.,
BanerjeeR.2015; Long-term care facilities are reservoirs for antimicrobial-resistant sequence type 131 Escherichia coli
. Open Forum Infect Dis 2:ofv011 [View Article][PubMed]
DahbiG.,
MoraA.,
MamaniR.,
LópezC.,
AlonsoM. P.,
MarzoaJ.,
BlancoM.,
HerreraA.,
VisoS.,
other authors.
2014; Molecular epidemiology and virulence of Escherichia coli O16:H5-ST131: comparison with H30 and H30-Rx subclones of O25b:H4-ST131. Int J Med Microbiol 304:1247–1257 [View Article][PubMed]
DashtiA. A.,
ValiL.,
El-ShazlyS.,
JadaonM. M.2014; The characterization and antibiotic resistance profiles of clinical Escherichia coli O25b-B2-ST131 isolates in Kuwait. BMC Microbiol 14:214 [View Article][PubMed]
EjrnæsK.2011; Bacterial characteristics of importance for recurrent urinary tract infections caused by Escherichia coli
. Dan Med Bull 58:B4187[PubMed]
FamN.,
Leflon-GuiboutV.,
FouadS.,
Aboul-FadlL.,
MarconE.,
DesoukyD.,
El-DefrawyI.,
Abou-AittaA.,
KlenaJ.,
Nicolas-ChanoineM. H.2011; CTX-M-15-producing Escherichia coli clinical isolates in Cairo (Egypt), including isolates of clonal complex ST10 and clones ST131, ST73, and ST405 in both community and hospital settings. Microb Drug Resist 17:67–73 [View Article][PubMed]
HoP. L.,
ChuY. P.,
LoW. U.,
ChowK. H.,
LawP. Y.,
TseC. W.,
NgT. K.,
ChengV. C.,
QueT. L.2015; High prevalence of Escherichia coli sequence type 131 among antimicrobial-resistant E. coli isolates from geriatric patients. J Med Microbiol 64:243–247 [View Article][PubMed]
JohnsonA. P.,
WoodfordN.2013; Global spread of antibiotic resistance: the example of New Delhi metallo-β-lactamase (NDM)-mediated carbapenem resistance. J Med Microbiol 62:499–513 [View Article][PubMed]
LanzaV. F.,
de ToroM.,
Garcillán-BarciaP.,
MoraA.,
BlancoJ.,
CoqueT. M.,
de la CruzF.2014; Plasmid flux in Escherichia coli ST131 sublineages, analyzed by Plasmid Constellation Network (PLACNET), a new method for plasmid reconstruction from whole genome sequences. PLoS Genetics 10:e1004766[CrossRef]
Leflon-GuiboutV.,
JurandC.,
BonacorsiS.,
EspinasseF.,
GuelfiM. C.,
DuportailF.,
HeymB.,
BingenE.,
Nicolas-ChanoineM. H.2004; Emergence and spread of three clonally related virulent isolates of CTX-M-15-producing Escherichia coli with variable resistance to aminoglycosides and tetracycline in a French geriatric hospital. Antimicrob Agents Chemother 48:3736–3742 [View Article][PubMed]
MirzaeeM.,
OwliaP.,
MansouriS.2009; Distribution of CTX-M β-lactamase genes among Escherichia coli strains isolated from patients in Iran. Lab Med 40:724–727 [View Article]
NielsenK. L.,
DynesenP.,
LarsenP.,
Frimodt-MøllerN.2014; Faecal Escherichia coli from patients with E. coli urinary tract infection and healthy controls who have never had a urinary tract infection. J Med Microbiol 63:582–589 [View Article][PubMed]
PapagiannitsisC. C.,
ŠtudentováV.,
Jakubu˚V.,
ŠpaneˇlováP.,
UrbáškováP.,
ZˇemličkováH.,
HrabákJ.2015; High prevalence of ST131 among CTX-M-producing Escherichia coli from community-acquired infections, in the Czech Republic. Microb Drug Resist 21:74–84 [View Article][PubMed]
PeiranoG.,
van der BijA. K.,
FreemanJ. L.,
PoirelL.,
NordmannP.,
CostelloM.,
TchesnokovaV. L.,
PitoutJ. D.2014; Characteristics of Escherichia coli sequence type 131 isolates that produce extended-spectrum β-lactamases: global distribution of the H30-Rx sublineage. Antimicrob Agents Chemother 58:3762–3767 [View Article][PubMed]
PettyN. K.,
Ben ZakourN. L.,
Stanton-CookM.,
SkippingtonE.,
TotsikaM.,
FordeB. M.,
PhanM. D.,
Gomes MorielD.,
PetersK. M.,
other authors.
2014; Global dissemination of a multidrug resistant Escherichia coli clone. Proc Natl Acad Sci U S A 111:5694–5699 [View Article][PubMed]
QureshiZ. A.,
DoiY.2014; Escherichia coli type 131. Epidemiology and challenges in treatment. Exper Rev Anti Infect Ther 12:597–609 [View Article][PubMed]