RT Journal Article SR Electronic(1) A1 Lay, Christophe Le A1 Dridi, Larbi A1 Bergeron, Michel G. A1 Ouellette, Marc A1 Fliss, Ismaı¨lYR 2016 T1 Nisin is an effective inhibitor of Clostridium difficile vegetative cells and spore germination JF Journal of Medical Microbiology, VO 65 IS 2 SP 169 OP 175 DO https://doi.org/10.1099/jmm.0.000202 PB Microbiology Society, SN 1473-5644, AB Clostridium difficile is the most frequently identified enteric pathogen in patients with nosocomial antibiotic-associated diarrhoea and pseudomembranous colitis. Several clinically isolated C. difficile strains are resistant to antibiotics other than metronidazole and vancomycin. Recently, bacteriocins of lactic acid bacteria have been proposed as an alternative or complementary treatment. The aim of this study was to investigate the inhibitory effect of nisin, a bacteriocin produced by several strains of Lactococcus lactis, against clinical isolates of C. difficile. Nisin Z obtained from culture of L. lactis subsp. lactis biovar. diacetylactis was tested along with commercial nisin A. The effect of nisin A on C. difficile spores was also examined. Nisin A and Z both inhibited the growth of all C. difficile isolates, and MICs were estimated at 6.2 μg ml− 1 for nisin Z and 0.8 μg ml− 1 for nisin A. In addition, C. difficile spores were also susceptible to nisin A (25.6 μg ml− 1), which reduced spore viability by 40–50 %. These results suggested that nisin and hence nisin-producing Lactococcus strains could be used to treat C. difficile-associated diarrhoea., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000202