1887

Abstract

is considered one of the most important foodborne pathogens. The virulence-related proteins listeriolysin O (LLO) and p60 are critical factors involved in pathogenesis. In the present study, we investigated the effect of honokiol on LLO and p60 secreted from . A listeriolysin assay was used to investigate the haemolytic activities of exposed to honokiol, and the secretion of LLO and p60 was detected by immunoblot analysis. Additionally, the influence of honokiol on the transcription of LLO and p60 genes ( and , respectively) was analysed by real-time reverse transcription PCR. TNF-α release assays were performed to elucidate the biological relevance of changes in LLO and p60 secretion induced by honokiol. According to the data, honokiol showed good anti- activity, with MICs of 8–16 μg ml, and the secretion of LLO and p60 was decreased by honokiol. In addition, the transcription of and was inhibited by honokiol. Our results indicate that TNF-α production by RAW264.7 cells stimulated with supernatants was inhibited by honokiol. Based on these data, we propose that honokiol could be used as a promising natural compound against and its virulence factors.

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2015-12-01
2020-01-17
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