1887

Abstract

In the present study, the adhesion of bacteriocin-producing probiotic strains of onto extracellular matrix (ECM) proteins such as collagen and mucin and their potential to prevent pathogen invasion onto the ECM was ascertained. Fluorescence-based assays indicated that strains CRA21, CRA38 and CRA52 displayed considerable adhesion to ECM molecules, which was comparable to the probiotic GG. Flow cytometry-based quantitative assessment of the adhesion potential suggested that CRA21 exhibited superior adhesion onto the ECM as compared with other lactic acid bacteria strains. Furthermore, fluorescence-based assays suggested that the highest inhibition of adhesion onto collagen and mucin by bacteriocin-producing strains was observed in the exclusion mode as compared with the competition and displacement modes. This observation was supported by the higher binding affinity ( ) for the ECM exhibited by the strains as compared with . Interestingly, a crude plantaricin A extract from food isolates of displayed potent antibacterial activity on ECM-adhered cells. It is envisaged that the isolates displaying bacteriocinogenic and ECM-adhering traits can perhaps be explored to develop safe antibacterial therapeutic agents.

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2015-12-01
2019-10-18
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