1887

Abstract

The goal of this study was to compare biofilm synthesis among enterococci recovered from clinical samples (infection or colonization) of patients as well as environmental samples in order to determine possible virulence factors and clonal relationship. During a two-year period, clinical samples (blood, catheter tips, bronchial secretions, wounds, peritoneal fluid, urine) and rectal swabs collected from hospitalized patients as well as environmental water samples were tested for the presence of and . Antibiotic susceptibility testing was performed by the disc diffusion method and Etest. Strains were tested for the presence of , , , and genes by PCR. Clones were identified by PFGE (I). From infected patients, 48 strains were identified: 24 (10 -positive, 14 vancomycin-susceptible) and 24 (one -positive, 23 vancomycin-susceptible). Among 143 colonizing isolates, 134 were (58 -positive, 11 -positive, 65 vancomycin-susceptible) and nine (three -positive, two -positive, four vancomycin-susceptible). Among 167 environmental water samples, 51 and 19 isolates, all glycopeptide-susceptible, were recovered. In total, 64 strains produced biofilm, whereas 34 were -positive, 64 -positive and 54 -positive. Biofilm production was associated with the presence of ( < 0.001) and genes ( = 0.021), being higher in infecting ( < 0.001) and water ( 0.005) isolates as compared with colonizing ones. Clones of environmental water-strains were different than the patients' clones. The differences found in the incidence of antibiotic resistance, virulence factors and clones suggest that hospital and water enterococci are of different origin.

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2015-11-01
2019-12-11
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