1887

Abstract

Toxoplasmosis is one of the most common parasitic diseases worldwide and it poses a serious challenge regarding prevention, diagnosis and therapy. The commonly used diagnostic methods are mostly based on the detection of specific antibodies in sera. Since they are not always accurate enough and do not allow precise definition of the phase of the infection, there is an urgent need to find specific molecular markers of acute or chronic infection stages. This study provides a comparative assessment of recombinant ROP5 and ROP18 proteins in the serodiagnosis of human toxoplasmosis. We found that both ROP5 and ROP18 proteins allowed the detection of specific IgM and IgG antibodies with a relatively low sensitivity; however, ROP18 IgM ELISA proved to be more sensitive than the SAG1 assay. This study also points to a relatively weak potential of the corresponding native ROP5 and ROP18 kinases in the generation of a strong antibody response in humans.

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2015-10-01
2019-10-13
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References

  1. Aubert D. , Maine G. T. , Villena I. , Hunt J. C. , Howard L. , Sheu M. , Brojanac S. , Chovan L. E. , Nowlan S. F. , Pinon J. M. . ( 2000;). Recombinant antigens to detect Toxoplasma gondii-specific immunoglobulin G and immunoglobulin M in human sera by enzyme immunoassay. J Clin Microbiol 38: 1144–1150.
    [Google Scholar]
  2. Behnke M. S. , Fentress S. J. , Mashayekhi M. , Li L. X. , Taylor G. A. , Sibley L. D. . ( 2012;). The polymorphic pseudokinase ROP5 controls virulence in Toxoplasma gondii by regulating the active kinase ROP18. PLoS Pathog 8: e1002992 [CrossRef] [PubMed].
    [Google Scholar]
  3. Chang P. Y. , Fong M. Y. , Nissapatorn V. , Lau Y. L. . ( 2011;). Evaluation of Pichia pastoris-expressed recombinant rhoptry protein 2 of Toxoplasma gondii for its application in diagnosis of toxoplasmosis. Am J Trop Med Hyg 85: 485–489 [CrossRef] [PubMed].
    [Google Scholar]
  4. Ciardelli L. , Meroni V. , Avanzini M. A. , Bollani L. , Tinelli C. , Garofoli F. , Gasparoni A. , Stronati M. . ( 2008;). Early and accurate diagnosis of congenital toxoplasmosis. Pediatr Infect Dis J 27: 125–129 [PubMed].
    [Google Scholar]
  5. Contini C. . ( 2008;). Clinical and diagnostic management of toxoplasmosis in the immunocompromised patient. Parassitologia 50: 45–50 [PubMed].
    [Google Scholar]
  6. Drapała D. , Holec-Ga˛sior L. , Kur J. . ( 2015;). New recombinant chimeric antigens, P35-MAG1, MIC1-ROP1, and MAG1-ROP1, for the serodiagnosis of human toxoplasmosis. Diagn Microbiol Infect Dis 82: 34–39 [CrossRef] [PubMed].
    [Google Scholar]
  7. Dziadek B. , Gatkowska J. , Brzostek A. , Dziadek J. , Dzitko K. , Długońska H. . ( 2009;). Toxoplasma gondii: the immunogenic and protective efficacy of recombinant ROP2 and ROP4 rhoptry proteins in murine experimental toxoplasmosis. Exp Parasitol 123: 81–89 [CrossRef] [PubMed].
    [Google Scholar]
  8. Dziadek B. , Gatkowska J. , Brzostek A. , Dziadek J. , Dzitko K. , Grzybowski M. , Dlugonska H. . ( 2011;). Evaluation of three recombinant multi-antigenic vaccines composed of surface and secretory antigens of Toxoplasma gondii in murine models of experimental toxoplasmosis. Vaccine 29: 821–830 [CrossRef] [PubMed].
    [Google Scholar]
  9. Etheridge R. D. , Alaganan A. , Tang K. , Lou H. J. , Turk B. E. , Sibley L. D. . ( 2014;). The Toxoplasma pseudokinase ROP5 forms complexes with ROP18 and ROP17 kinases that synergize to control acute virulence in mice. Cell Host Microbe 15: 537–550 [CrossRef] [PubMed].
    [Google Scholar]
  10. Gatkowska J. , Hiszczyńska-Sawicka E. , Kur J. , Holec L. , Dlugonska H. . ( 2006;). Toxoplasma gondii: an evaluation of diagnostic value of recombinant antigens in a murine model. Exp Parasitol 114: 220–227 [CrossRef] [PubMed].
    [Google Scholar]
  11. Gatkowska J. , Dziadek B. , Brzostek A. , Dziadek J. , Dzitko K. , Długońska H. . ( 2010;). Determination of diagnostic value of Toxoplasma gondii recombinant ROP2 and ROP4 antigens in mouse experimental model. Pol J Microbiol 59: 137–141 [PubMed].[CrossRef]
    [Google Scholar]
  12. Glor S. B. , Edelhofer R. , Grimm F. , Deplazes P. , Basso W. . ( 2013;). Evaluation of a commercial ELISA kit for detection of antibodies against Toxoplasma gondii in serum, plasma and meat juice from experimentally and naturally infected sheep. Parasit Vectors 6: 85 [CrossRef] [PubMed].
    [Google Scholar]
  13. Grzybowski M. M. , Dziadek B. , Dziadek J. , Gatkowska J. , Dzitko K. , Długońska H. . ( 2015;). Toxoplasma gondii: cloning, expression and immunoreactivity of recombinant ROP5 and ROP18 antigens. Exp Parasitol 150: 1–6 [CrossRef] [PubMed].
    [Google Scholar]
  14. Hill D. , Dubey J. P. . ( 2002;). Toxoplasma gondii: transmission, diagnosis and prevention. Clin Microbiol Infect 8: 634–640 [CrossRef] [PubMed].
    [Google Scholar]
  15. Holec-Ga˛sior L. . ( 2013;). Toxoplasma gondii recombinant antigens as tools for serodiagnosis of human toxoplasmosis: current status of studies. Clin Vaccine Immunol 20: 1343–1351 [CrossRef] [PubMed].
    [Google Scholar]
  16. Holec-Ga˛sior L. , Kur J. , Hiszczyńska-Sawicka E. . ( 2009;). GRA2 and ROP1 recombinant antigens as potential markers for detection of Toxoplasma gondii-specific immunoglobulin G in humans with acute toxoplasmosis. Clin Vaccine Immunol 16: 510–514 [CrossRef] [PubMed].
    [Google Scholar]
  17. Jacobs D. , Vercammen M. , Saman E. . ( 1999;). Evaluation of recombinant dense granule antigen 7 (GRA7) of Toxoplasma gondii for detection of immunoglobulin G antibodies and analysis of a major antigenic domain. Clin Diagn Lab Immunol 6: 24–29 [PubMed].
    [Google Scholar]
  18. Jensen K. D. , Camejo A. , Melo M. B. , Cordeiro C. , Julien L. , Grotenbreg G. M. , Frickel E. M. , Ploegh H. L. , Young L. , Saeij J. P. . ( 2015;). Toxoplasma gondii superinfection and virulence during secondary infection correlate with the exact ROP5/ROP18 allelic combination. MBio 6: e02280–e02214 [CrossRef] [PubMed].
    [Google Scholar]
  19. Jones J. L. , Dubey J. P. . ( 2012;). Foodborne toxoplasmosis. Clin Infect Dis 55: 845–851 [CrossRef] [PubMed].
    [Google Scholar]
  20. Kieffer F. , Wallon M. . ( 2013;). Congenital toxoplasmosis. Handb Clin Neurol 112: 1099–1101 [CrossRef] [PubMed].
    [Google Scholar]
  21. Kotresha D. , Noordin R. . ( 2010;). Recombinant proteins in the diagnosis of toxoplasmosis. APMIS 118: 529–542 [PubMed].
    [Google Scholar]
  22. Martin V. , Arcavi M. , Santillan G. , Amendoeira M. R. , De Souza Neves E. , Griemberg G. , Guarnera E. , Garberi J. C. , Angel S. O. . ( 1998;). Detection of human Toxoplasma-specific immunoglobulins A, M, and G with a recombinant Toxoplasma gondii rop2 protein. Clin Diagn Lab Immunol 5: 627–631 [PubMed].
    [Google Scholar]
  23. Meek B. , van Gool T. , Gilis H. , Peek R. . ( 2001;). Dissecting the IgM antibody response during the acute and latent phase of toxoplasmosis. Diagn Microbiol Infect Dis 41: 131–137 [CrossRef] [PubMed].
    [Google Scholar]
  24. Montoya J. G. . ( 2002;). Laboratory diagnosis of Toxoplasma gondii infection and toxoplasmosis. J Infect Dis 185: (Suppl. 1), S73–S82 [CrossRef] [PubMed].
    [Google Scholar]
  25. Murat J. B. , Hidalgo H. F. , Brenier-Pinchart M. P. , Pelloux H. . ( 2013;). Human toxoplasmosis: which biological diagnostic tests are best suited to which clinical situations?. Expert Rev Anti Infect Ther 11: 943–956 [CrossRef] [PubMed].
    [Google Scholar]
  26. Niedelman W. , Gold D. A. , Rosowski E. E. , Sprokholt J. K. , Lim D. , Farid Arenas A. , Melo M. B. , Spooner E. , Yaffe M. B. , Saeij J. P. . ( 2012;). The rhoptry proteins ROP18 and ROP5 mediate Toxoplasma gondii evasion of the murine, but not the human, interferon-gamma response. PLoS Pathog 8: e1002784 [CrossRef] [PubMed].
    [Google Scholar]
  27. Okeh U. M. , Ogbonna L. N. . ( 2013;). Statistical evaluation of indicators of diagnostic test performance. Am J Biosci 1: 63–73 [CrossRef].
    [Google Scholar]
  28. Oz H. S. . ( 2014;). Maternal and congenital toxoplasmosis, currently available and novel therapies in horizon. Front Microbiol 5: 385 [CrossRef] [PubMed].
    [Google Scholar]
  29. Pfrepper K. I. , Enders G. , Gohl M. , Krczal D. , Hlobil H. , Wassenberg D. , Soutschek E. . ( 2005;). Seroreactivity to and avidity for recombinant antigens in toxoplasmosis. Clin Diagn Lab Immunol 12: 977–982 [PubMed].
    [Google Scholar]
  30. Reese M. L. , Zeiner G. M. , Saeij J. P. J. , Boothroyd J. C. , Boyle J. P. . ( 2011;). Polymorphic family of injected pseudokinases is paramount in Toxoplasma virulence. Proc Natl Acad Sci U S A 108: 9625–9630 [CrossRef] [PubMed].
    [Google Scholar]
  31. Robert-Gangneux F. , Sterkers Y. , Yera H. , Accoceberry I. , Menotti J. , Cassaing S. , Brenier-Pinchart M. P. , Hennequin C. , Delhaes L. , other authors . ( 2015;). Molecular diagnosis of toxoplasmosis in immunocompromised patients: a 3-year multicenter retrospective study. J Clin Microbiol 53: 1677–1684 [CrossRef] [PubMed].
    [Google Scholar]
  32. Roux-Buisson N. , Fricker-Hidalgo H. , Foussadier A. , Rolland D. , Suchel-Jambon A.-S. , Brenier-Pinchart M.-P. , Pelloux H. . ( 2005;). Comparative analysis of the VIDAS Toxo IgG IV assay in the detection of antibodies to Toxoplasma gondii . Diagn Microbiol Infect Dis 53: 79–81 [CrossRef] [PubMed].
    [Google Scholar]
  33. Saavedra R. , de Meuter F. , Decourt J. L. , Hérion P. . ( 1991;). Human T cell clone identifies a potentially protective 54-kDa protein antigen of Toxoplasma gondii cloned and expressed in Escherichia coli . J Immunol 147: 1975–1982 [PubMed].
    [Google Scholar]
  34. Sambrook J. , Russell D. W. . ( 2001;). Molecular Cloning: a Laboratory Manual , 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Press;.
    [Google Scholar]
  35. Sánchez V. , de-la-Torre A. , Gómez-Marín J. E. . ( 2014;). Characterization of ROP18 alleles in human toxoplasmosis. Parasitol Int 63: 463–469 [CrossRef] [PubMed].
    [Google Scholar]
  36. Sheffield P. , Garrard S. , Derewenda Z. . ( 1999;). Overcoming expression and purification problems of RhoGDI using a family of parallel expression vectors. Protein Expr Purif 15: 34–39 [CrossRef] [PubMed].
    [Google Scholar]
  37. Sonaimuthu P. , Fong M. Y. , Kalyanasundaram R. , Mahmud R. , Lau Y. L. . ( 2014;). Sero-diagnostic evaluation of Toxoplasma gondii recombinant Rhoptry antigen 8 expressed in E. coli . Parasit Vectors 7: 297 [CrossRef] [PubMed].
    [Google Scholar]
  38. Tan T. G. , Mui E. , Cong H. , Witola W. H. , Montpetit A. , Muench S. P. , Sidney J. , Alexander J. , Sette A. , other authors . ( 2010;). Identification of T. gondii epitopes, adjuvants, and host genetic factors that influence protection of mice and humans. Vaccine 28: 3977–3989 [CrossRef] [PubMed].
    [Google Scholar]
  39. Torres-Morales E. , Taborda L. , Cardona N. , De-la-Torre A. , Sepulveda-Arias J. C. , Patarroyo M. A. , Gomez-Marin J. E. . ( 2014;). Th1 and Th2 immune response to P30 and ROP18 peptides in human toxoplasmosis. Med Microbiol Immunol (Berl) 203: 315–322 [CrossRef] [PubMed].
    [Google Scholar]
  40. van Gelder P. , Bosman F. , de Meuter F. , van Heuverswyn H. , Hérion P. . ( 1993;). Serodiagnosis of toxoplasmosis by using a recombinant form of the 54-kilodalton rhoptry antigen expressed in Escherichia coli . J Clin Microbiol 31: 9–15 [PubMed].
    [Google Scholar]
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