activity of minocycline alone or in combination in multidrug-resistant isolates Free

Abstract

Minocycline (MIN) usually shows good activity against strains. The reintroduction to the market of intravenous MIN provides an additional agent to the limited options for the treatment of infections. The activity of MIN as a single agent and in combination with rifampicin (RIF), colistin (COL) or imipenem (IMI) was evaluated by means of killing curves and 24 h-time-kill curves in five isolates which were selected on the basis of different antimicrobial resistance profiles. MIN showed bacteriostatic activity in three isolates (2 ×  or 16 ×  MIC) and bactericidal activity in the other isolates (64 ×  MIC). In isolates harbouring the gene, the associations studied were always indifferent. However, in isolates not harbouring , the use of MIN in combination showed a rapid synergistic effect (at 4 h) in four out of nine combinations (two with RIF and one each with IMI and COL). At 24 h, this effect was observed in six out of nine combinations (two in each association). MIN in combination with RIF, IMI and COL showed bactericidal synergy in most of the isolates which did not harbour the gene, but the combinations were not synergistic in -positive isolates.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000147
2015-10-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/64/10/1196.html?itemId=/content/journal/jmm/10.1099/jmm.0.000147&mimeType=html&fmt=ahah

References

  1. Bantar C., Schell C., Posse G., Limansky A., Ballerini V., Mobilia L. 2008; Comparative time-kill study of doxycycline, tigecycline, sulbactam, and imipenem against several clones of Acinetobacter baumannii . Diagn Microbiol Infect Dis 61:309–314 [View Article][PubMed]
    [Google Scholar]
  2. Castanheira M., Mendes R. E., Jones R. N. 2014; Update on Acinetobacter species: mechanisms of antimicrobial resistance and contemporary in vitro activity of minocycline and other treatment options. Clin Infect Dis 59:(Suppl 6)S367–S373 [View Article][PubMed]
    [Google Scholar]
  3. CLSI 2012 Performance Standards for Antimicrobial Susceptibility Testing 22nd Informational Supplement M100-S22. Wayne, PA: Clinical and Laboratory Standards Institute;
    [Google Scholar]
  4. Goff D. A., Kaye K. S. 2014; Minocycline: an old drug for a new bug: multidrug-resistant Acinetobacter baumannii . Clin Infect Dis 59:(Suppl 6)S365–S366 [View Article][PubMed]
    [Google Scholar]
  5. Huys G., Cnockaert M., Vaneechoutte M., Woodford N., Nemec A., Dijkshoorn L., Swings J. 2005; Distribution of tetracycline resistance genes in genotypically related and unrelated multiresistant Acinetobacter baumannii strains from different European hospitals. Res Microbiol 156:348–355 [View Article][PubMed]
    [Google Scholar]
  6. Li J., Rayner C. R., Nation R. L., Owen R. J., Spelman D., Tan K. E., Liolios L. 2006; Heteroresistance to colistin in multidrug-resistant Acinetobacter baumannii . Antimicrob Agents Chemother 50:2946–2950 [View Article][PubMed]
    [Google Scholar]
  7. Liang W., Liu X. F., Huang J., Zhu D. M., Li J., Zhang J. 2011; Activities of colistin- and minocycline-based combinations against extensive drug resistant Acinetobacter baumannii isolates from intensive care unit patients. BMC Infect Dis 11:109–116 [View Article][PubMed]
    [Google Scholar]
  8. Members of the SFM Antibiogram Committee 2003; Comité de l'Antibiogramme de la Société Française de Microbiologie report 2003. Int J Antimicrob Agents 21:364–391 [CrossRef]
    [Google Scholar]
  9. Peleg A. Y., Seifert H., Paterson D. L. 2008; Acinetobacter baumannii: emergence of a successful pathogen. Clin Microbiol Rev 21:538–582 [View Article][PubMed]
    [Google Scholar]
  10. Poirel L., Walsh T. R., Cuvillier V., Nordmann P. 2011; Multiplex PCR for detection of acquired carbapenemase genes. Diagn Microbiol Infect Dis 70:119–123 [View Article][PubMed]
    [Google Scholar]
  11. Ritchie D. J., Garavaglia-Wilson A. 2014; A review of intravenous minocycline for treatment of multidrug-resistant Acinetobacter infections. Clin Infect Dis 59:(Suppl 6)S374–S380 [View Article][PubMed]
    [Google Scholar]
  12. Rodriguez C. H., De Ambrosio A., Bajuk M., Spinozzi M., Nastro M., Bombicino K., Radice M., Gutkind G., Vay C., Famiglietti A. 2010; In vitro antimicrobials activity against endemic Acinetobacter baumannii multiresistant clones. J Infect Dev Ctries 4:164–167 [View Article][PubMed]
    [Google Scholar]
  13. Rodríguez-Hernández M. J., Pachón J., Pichardo C., Cuberos L., Ibáñez-Martínez J., García-Curiel A., Caballero F. J., Moreno I., Jiménez-Mejías M. E. 2000; Imipenem, doxycycline and amikacin in monotherapy and in combination in Acinetobacter baumannii experimental pneumonia. J Antimicrob Chemother 45:493–501 [View Article][PubMed]
    [Google Scholar]
  14. Seifert H., Dolzani L., Bressan R., van der Reijden T., van Strijen B., Stefanik D., Heersma H., Dijkshoorn L. 2005; Standardization and interlaboratory reproducibility assessment of pulsed-field gel electrophoresis-generated fingerprints of Acinetobacter baumannii . J Clin Microbiol 43:4328–4335 [View Article][PubMed]
    [Google Scholar]
  15. Tan T. Y., Ng L. S., Tan E., Huang G. 2007; In vitro effect of minocycline and colistin combinations on imipenem-resistant Acinetobacter baumannii clinical isolates. J Antimicrob Chemother 60:421–423 [View Article][PubMed]
    [Google Scholar]
  16. Wood G. C., Hanes S. D., Boucher B. A., Croce M. A., Fabian T. C. 2003; Tetracyclines for treating multidrug-resistant Acinetobacter baumannii ventilator-associated pneumonia. Intensive Care Med 29:2072–2076 [View Article][PubMed]
    [Google Scholar]
  17. Woodford N., Ellington M. J., Coelho J. M., Turton J. F., Ward M. E., Brown S., Amyes S. G., Livermore D. M. 2006; Multiplex PCR for genes encoding prevalent OXA carbapenemases in Acinetobacter spp. Int J Antimicrob Agents 27:351–353 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.000147
Loading
/content/journal/jmm/10.1099/jmm.0.000147
Loading

Data & Media loading...

Most cited Most Cited RSS feed