1887

Abstract

The epidemiology of nocardiosis is evolving with increasing number of spp. causing human infection. In recent years, molecular techniques have been used to identify spp. There are limited data available on the spectrum of spp. isolated from clinical samples in India. Here, a molecular study was carried on 30 clinical isolates maintained in our National Culture Collection to evaluate the techniques used for identifying the agents. The isolates were identified by sequencing two promising genes: the 16S rRNA gene and . Both and the 16S rRNA gene could reliably identify 90 % of isolates, i.e. , , , , and . The mean percentage dissimilarity of sequence identification was higher using the gene (4 %, range 0–7.9 %) compared with the 16S rRNA gene (2.3 %, range 0–8.9 %). Two isolates that showed ambiguous results in both the short segment of the 16S rRNA gene and sequences could be resolved by sequencing a larger fragment (∼1000 bp) of the 16S rRNA gene. Both of these isolates were identified as with similarities of 99.8 and 100 % compared with the standard strain. Genotyping of strains was performed using gene sequences and compared with previously described genotypes. Our isolates belonged to genotype 1 ( = 4) and genotype 2 ( = 2). The present study highlights a wide spectrum of spp. in India and emphasizes the need for molecular techniques for identification to the species level.

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2015-10-01
2019-12-05
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