1887

Abstract

Yeasts of the genera and are opportunist pathogens and cause oral lesions, especially in immunocompromised patients. This study assessed yeasts isolated from chronic kidney patients undergoing haemodialysis for their adhesion capacity, biofilm formation and susceptibility to antifungal agents. Ten isolates of spp. and one isolate of were tested for adhesion to buccal epithelial cells (BECs), adhesion and formation of biofilm in artificial saliva and their susceptibility profile to antifungal agents. Adhesion and biofilm formation were undertaken in polystyrene plates with artificial saliva, whilst susceptibility to antifungal agents was evaluated by broth microdilution. had the highest adhesion index in BECs (154.55 ± 22.13) and was the species with the highest adhesion capacity (18 398 Abs cm) in abiotic surface with artificial saliva. provided the greatest biofilm formation (2035 Abs cm ± 0.09) but was revealed to be susceptible to the five antifungal agents under analysis. However, some non- isolates showed a lower susceptibility for the antifungal agents itraconazole, fluconazole and voriconazole. All of the species were sensitive to amphotericin B and nystatin. The current analysis showed that yeasts isolated from the mouth of chronic kidney patients undergoing haemodialysis varied significantly with regard to their capacity for adherence, biofilm formation and susceptibility to antifungal agents, underscoring the high virulence of non- species.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000122
2015-09-01
2020-01-21
Loading full text...

Full text loading...

/deliver/fulltext/jmm/64/9/960.html?itemId=/content/journal/jmm/10.1099/jmm.0.000122&mimeType=html&fmt=ahah

References

  1. Bassetti M., Ansaldi F., Nicolini L., Malfatto E., Molinari M. P., Mussap M., Rebesco B., Bobbio Pallavicini F., Icardi G., Viscoli C.. ( 2009;). Incidence of candidaemia and relationship with fluconazole use in an intensive care unit. J Antimicrob Chemother 64: 625–629 [CrossRef] [PubMed].
    [Google Scholar]
  2. Bendel C. M., Hostetter M. K.. ( 1993;). Distinct mechanisms of epithelial adhesion for Candida albicans and Candida tropicalis. Identification of the participating ligands and development of inhibitory peptides. J Clin Invest 92: 1840–1849 [CrossRef] [PubMed].
    [Google Scholar]
  3. Carlén A., Nikdel K., Wennerberg A., Holmberg K., Olsson J.. ( 2001;). Surface characteristics and in vitro biofilm formation on glass ionomer and composite resin. Biomaterials 22: 481–487 [CrossRef] [PubMed].
    [Google Scholar]
  4. Carrillo-Muñoz A. J., Quindós G., Tur C., Ruesga M. T., Miranda Y., del Valle O., Cossum P. A., Wallace T. L.. ( 1999;). In-vitro antifungal activity of liposomal nystatin in comparison with nystatin, amphotericin B cholesteryl sulphate, liposomal amphotericin B, amphotericin B lipid complex, amphotericin B desoxycholate, fluconazole and itraconazole. J Antimicrob Chemother 44: 397–401 [CrossRef] [PubMed].
    [Google Scholar]
  5. Chauhan N. M., Shinde R. B., Karuppayil S. M.. ( 2013;). Effect of alcohols on filamentation, growth, viability and biofilm development in Candida albicans. Braz J Microbiol 44: 1315–1320 [CrossRef] [PubMed].
    [Google Scholar]
  6. Choukri F., Benderdouche M., Sednaoui P.. ( 2014;). In vitro susceptibility profile of 200 recent clinical isolates of Candida spp. to topical antifungal treatments of vulvovaginal candidiasis, the imidazoles and nystatin agents. J Mycol Med 24: 303–307 [CrossRef] [PubMed].
    [Google Scholar]
  7. CLSI ( 2008;). Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved standard M27–A3, 3rd edn. Wayne, PA: Clinical and Laboratory Standards Institute;.
    [Google Scholar]
  8. Conde-Rosa A., Amador R., Pérez-Torres D., Colón E., Sánchez-Rivera C., Nieves-Plaza M., González-Ramos M., Bertrán-Pasarell J.. ( 2010;). Candidemia distribution, associated risk factors, and attributed mortality at a university-based medical center. P R Health Sci J 29: 26–29.
    [Google Scholar]
  9. Córdoba S., Vivot W., Bosco-Borgeat M. E., Taverna C., Szusz W., Murisengo O., Isla G., Davel G., Red Nacional De Laboratorios De Micologia. ( 2011;). Species distribution and susceptibility profile of yeasts isolated from blood cultures: results of a multicenter active laboratory-based surveillance study in Argentina. Rev Argent Microbiol 43: 176–185.
    [Google Scholar]
  10. Cuéllar-Cruz M., Vega-González A., Mendoza-Novelo B., López-Romero E., Ruiz-Baca E., Quintanar-Escorza M. A., Villagómez-Castro J. C.. ( 2012;). The effect of biomaterials and antifungals on biofilm formation by Candida species: a review. Eur J Clin Microbiol Infect Dis 31: 2513–2527 [CrossRef] [PubMed].
    [Google Scholar]
  11. Enache-Angoulvant A., Hennequin C.. ( 2005;). Invasive Saccharomyces infection: a comprehensive review. Clin Infect Dis 41: 1559–1568 [CrossRef] [PubMed].
    [Google Scholar]
  12. Eren Z., Gurol Y., Sonmezoglu M., Eren H. S., Celik G., Kantarci G.. ( 2014;). [Saccharomyces cerevisiae fungemia in an elderly patient following probiotic treatment]. Mikrobiyol Bul 48: 351–355 (in Turkish) [CrossRef] [PubMed].
    [Google Scholar]
  13. Furlaneto M. C., Rota J. F., Quesada R. M., Furlaneto-Maia L., Rodrigues R., Oda S., Oliveira M. T., Serpa R., França E. J.. ( 2011;). Species distribution and in vitro fluconazole susceptibility of clinical Candida isolates in a Brazilian tertiary-care hospital over a 3-year period. Rev Soc Bras Med Trop 44: 595–599 [CrossRef] [PubMed].
    [Google Scholar]
  14. Godoy J. S., de Souza Bonfim-Mendonça P., Nakamura S. S., Yamada S. S., Shinobu-Mesquita C., Pieralisi N., Fiorini A., Svidzinski T. I.. ( 2013;). Colonization of the oral cavity by yeasts in patients with chronic renal failure undergoing hemodialysis. J Oral Pathol Med 42: 229–234 [CrossRef] [PubMed].
    [Google Scholar]
  15. Guinea J.. ( 2014;). Global trends in the distribution of Candida species causing candidemia. Clin Microbiol Infect 20: (Suppl 6), 5–10 [CrossRef] [PubMed].
    [Google Scholar]
  16. Hamza O. J., Matee M. I., Moshi M. J., Simon E. N., Mugusi F., Mikx F. H., Helderman W. H., Rijs A. J., van der Ven A. J., Verweij P. E.. ( 2008;). Species distribution and in vitro antifungal susceptibility of oral yeast isolates from Tanzanian HIV-infected patients with primary and recurrent oropharyngeal candidiasis. BMC Microbiol 8: 135 [CrossRef] [PubMed].
    [Google Scholar]
  17. Jensen J., Muñoz P., Guinea J., Rodríguez-Créixems M., Peláez T., Bouza E.. ( 2007;). Mixed fungemia: incidence, risk factors, and mortality in a general hospital. Clin Infect Dis 44: e109–e114 [CrossRef] [PubMed].
    [Google Scholar]
  18. Kuhn D. M., George T., Chandra J., Mukherjee P. K., Ghannoum M. A.. ( 2002;). Antifungal susceptibility of Candida biofilms: unique efficacy of amphotericin B lipid formulations and echinocandins. Antimicrob Agents Chemother 46: 1773–1780 [CrossRef] [PubMed].
    [Google Scholar]
  19. Montagna M. T., Lovero G., Coretti C., De Giglio O., Martinelli D., Bedini A., Delia M., Rosato A., Codeluppi M., Caggiano G.. ( 2014;). In vitro activities of amphotericin B deoxycholate and liposomal amphotericin B against 604 clinical yeast isolates. J Med Microbiol 63: 1638–1643 [CrossRef] [PubMed].
    [Google Scholar]
  20. Montejo M.. ( 2011;). [Epidemiology of invasive fungal infection in solid organ transplant]. Rev Iberoam Micol 28: 120–123 (in Spanish) [CrossRef] [PubMed].
    [Google Scholar]
  21. Negri M., Martins M., Henriques M., Svidzinski T. I., Azeredo J., Oliveira R.. ( 2010;). Examination of potential virulence factors of Candida tropicalis clinical isolates from hospitalized patients. Mycopathologia 169: 175–182 [CrossRef] [PubMed].
    [Google Scholar]
  22. Nucci M., Queiroz-Telles F., Tobón A. M., Restrepo A., Colombo A. L.. ( 2010;). Epidemiology of opportunistic fungal infections in Latin America. Clin Infect Dis 51: 561–570 [CrossRef] [PubMed].
    [Google Scholar]
  23. Ortega M., Marco F., Soriano A., Almela M., Martínez J. A., López J., Pitart C., Mensa J.. ( 2011;). Candida species bloodstream infection: epidemiology and outcome in a single institution from 1991 to 2008. J Hosp Infect 77: 157–161 [CrossRef] [PubMed].
    [Google Scholar]
  24. Pfaller M. A., Diekema D. J., Procop G. W., Rinaldi M. G.. ( 2007;). Multicenter comparison of the VITEK 2 antifungal susceptibility test with the CLSI broth microdilution reference method for testing amphotericin B, flucytosine, and voriconazole against Candida spp. J Clin Microbiol 45: 3522–3528 [CrossRef] [PubMed].
    [Google Scholar]
  25. Pires-Gonçalves R. H., Miranda E. T., Baeza L. C., Matsumoto M. T., Zaia J. E., Mendes-Giannini M. J.. ( 2007;). Genetic relatedness of commensal strains of Candida albicans carried in the oral cavity of patients' dental prosthesis users in Brazil. Mycopathologia 164: 255–263 [CrossRef] [PubMed].
    [Google Scholar]
  26. Pizzo G., Giuliana G., Milici M. E., D'Angelo M.. ( 2001;). Effect of antimicrobial mouthrinses on the in vitro adhesion of Candida albicans to human buccal epithelial cells. Clin Oral Investig 5: 172–176 [CrossRef] [PubMed].
    [Google Scholar]
  27. Premkumar J., Ramani P., Chandrasekar T., Natesan A., Premkumar P.. ( 2014;). Detection of species diversity in oral candida colonization and anti-fungal susceptibility among non-oral habit adult diabetic patients. J Nat Sci Biol Med 5: 148–154 [CrossRef] [PubMed].
    [Google Scholar]
  28. Ribeiro E. L., Guimarães R. I., Inácio M. C. C., Ferreira W. M., Cardoso C. G., Dias S. M. S., Naves P. L. F.. ( 2004;). Aspectos das leveduras de Candida vinculadas às infecções nosocomiais. NewsLab 64: 106–128.
    [Google Scholar]
  29. Sardi J. C., Scorzoni L., Bernardi T., Fusco-Almeida A. M., Mendes Giannini M. J.. ( 2013;). Candida species: current epidemiology, pathogenicity, biofilm formation, natural antifungal products and new therapeutic options. J Med Microbiol 62: 10–24 [CrossRef] [PubMed].
    [Google Scholar]
  30. Sardi J. C., Pitangui N. S., Rodríguez-Arellanes G., Taylor M. L., Fusco-Almeida A. M., Mendes-Giannini M. J.. ( 2014;). Highlights in pathogenic fungal biofilms. Rev Iberoam Micol 31: 22–29 [CrossRef] [PubMed].
    [Google Scholar]
  31. Sarode N., Davis S. E., Tams R. N., Reynolds T. B.. ( 2014;). The Wsc1p cell wall signaling protein controls biofilm (Mat) formation independently of Flo11p in Saccharomyces cerevisiae. G3 (Bethesda) 4: 199–207 [CrossRef] [PubMed].
    [Google Scholar]
  32. Seabra C. L., Botelho C. M., Henriques M., Oliveira R.. ( 2013;). Differential adherence and expression of virulence traits by Candida albicans and Candida parapsilosis in mono- and dual-species cultures in artificial saliva. Mycopathologia 176: 33–40 [CrossRef] [PubMed].
    [Google Scholar]
  33. Serefhanoglu K., Timurkaynak F., Can F., Cagir U., Arslan H., Ozdemir F. N.. ( 2012;). Risk factors for candidemia with non-albicans Candida spp. in intensive care unit patients with end-stage renal disease on chronic hemodialysis. J Formos Med Assoc 111: 325–332 [CrossRef] [PubMed].
    [Google Scholar]
  34. Silva S., Henriques M., Martins A., Oliveira R., Williams D., Azeredo J.. ( 2009;). Biofilms of non-Candida albicans Candida species: quantification, structure and matrix composition. Med Mycol 47: 681–689 [CrossRef] [PubMed].
    [Google Scholar]
  35. Silva S., Negri M., Henriques M., Oliveira R., Williams D., Azeredo J.. ( 2010;). Silicone colonization by non-Candida albicans Candida species in the presence of urine. J Med Microbiol 59: 747–754 [CrossRef] [PubMed].
    [Google Scholar]
  36. Silva F. H. A., Paço F. R., Reis E., Amaral V.. ( 2011a;). Saccharomyces cerevisiae infection: an unusual pathogen in the ICU. Rev Bras Ter Intensiva 23: 108–111 [CrossRef] [PubMed].
    [Google Scholar]
  37. Silva S., Negri M., Henriques M., Oliveira R., Williams D. W., Azeredo J.. ( 2011b;). Adherence and biofilm formation of non-Candida albicans Candida species. Trends Microbiol 19: 241–247 [CrossRef] [PubMed].
    [Google Scholar]
  38. Silva M. P., Chibebe Junior J., Jorjão A. L., Machado A. K., Oliveira L. D., Junqueira J. C., Jorge A. O.. ( 2012;). Influence of artificial saliva in biofilm formation of Candida albicans in vitro. Braz Oral Res 26: 24–28 [CrossRef] [PubMed].
    [Google Scholar]
  39. Sohn K., Senyürek I., Fertey J., Königsdorfer A., Joffroy C., Hauser N., Zelt G., Brunner H., Rupp S.. ( 2006;). An in vitro assay to study the transcriptional response during adherence of Candida albicans to different human epithelia. FEMS Yeast Res 6: 1085–1093 [CrossRef] [PubMed].
    [Google Scholar]
  40. Taguti Irie M. M., Lopes Consolaro M. E., Aparecida Guedes T., Donatti L., Valéria Patussi E., Estivalet Svidzinski T. I.. ( 2006;). A simplified technique for evaluating the adherence of yeasts to human vaginal epithelial cells. J Clin Lab Anal 20: 195–203 [CrossRef] [PubMed].
    [Google Scholar]
  41. Tamura N. K., Negri M. F., Bonassoli L. A., Svidzinski T. I.. ( 2007;). [Virulence factors for Candida spp recovered from intravascular catheters and hospital workers hands]. Rev Soc Bras Med Trop 40: 91–93 (in Portuguese) [CrossRef] [PubMed].
    [Google Scholar]
  42. Weiss Nielsen M., Sternberg C., Molin S., Regenberg B.. ( 2011;). Pseudomonas aeruginosa and Saccharomyces cerevisiae biofilm in flow cells. J Vis Exp 47: e2383 [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.000122
Loading
/content/journal/jmm/10.1099/jmm.0.000122
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error